Release of Type 2 Cytokines by Epithelial Cells of Nasal Polyps
Background. T2 inflammation of chronic rhinosinusitis with nasal polyps (CRSwNP) may be influenced by epithelial cytokines release (TSLP, IL-25, and IL-33). We investigated the release of TSLP, IL-25, and IL-33 by epithelial CRSwNP cells compared to epithelial sinus mucosa cells of patients with chr...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2016/2643297 |
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| author | Monica Boita Caterina Bucca Giuseppe Riva Enrico Heffler Giovanni Rolla |
| author_facet | Monica Boita Caterina Bucca Giuseppe Riva Enrico Heffler Giovanni Rolla |
| author_sort | Monica Boita |
| collection | DOAJ |
| description | Background. T2 inflammation of chronic rhinosinusitis with nasal polyps (CRSwNP) may be influenced by epithelial cytokines release (TSLP, IL-25, and IL-33). We investigated the release of TSLP, IL-25, and IL-33 by epithelial CRSwNP cells compared to epithelial sinus mucosa cells of patients with chronic rhinosinusitis without nasal polyps (CRSsNP). Methods. IL-25, IL-33, and TSLP were measured by ELISA in the supernatant of cell cultures derived by CRSwNP (9 patients, 6 atopic) and CRSsNP (7 patients, 2 atopic) in baseline condition and following stimulation with Dermatophagoides pteronyssinus (DP), Aspergillus fumigatus (AF), and poly(I:C). Results. CRSwNP epithelial cells released increased levels of IL-25 (from 0.12 ± 0.06 pg/ml to 0.27 ± 0.1 pg/ml, p<0.01) and TSLP (from 0.77 ± 0.5 pg/ml to 2.53 ± 1.17 pg/ml, p<0.001) following poly(I:C) stimulation, while CRSsNP epithelial cells released increased levels of IL-25 and IL-33 following AF and DP stimulation, respectively (IL-25: from 0.18 ± 0.07 pg/ml to 0.51 ± 0.1 pg/ml, p<0.001; IL-33: from 2.57 ± 1.3 pg/ml to 5.7 ± 3.1 pg/ml, p<0.001). Conclusions. CRSwNP epithelial cells release TSLP and IL-25 when stimulated by poly(I:C) but not by DP or AF, suggesting that viral infection may contribute to maintain and amplify the T2 immune response seen in CRSwNP. |
| format | Article |
| id | doaj-art-8a518c570edb4f5194a68439016714cb |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-8a518c570edb4f5194a68439016714cb2025-02-03T01:25:57ZengWileyJournal of Immunology Research2314-88612314-71562016-01-01201610.1155/2016/26432972643297Release of Type 2 Cytokines by Epithelial Cells of Nasal PolypsMonica Boita0Caterina Bucca1Giuseppe Riva2Enrico Heffler3Giovanni Rolla4Department of Medical Sciences, University of Torino, Turin, ItalyDepartment of Medical Sciences, University of Torino, Turin, Italy1st ENT Division, Department of Surgical Sciences, University of Torino, Turin, ItalyRespiratory Medicine and Allergology, Department of Clinical and Experimental Medicine, University of Catania, Catania, ItalyDepartment of Medical Sciences, University of Torino, Turin, ItalyBackground. T2 inflammation of chronic rhinosinusitis with nasal polyps (CRSwNP) may be influenced by epithelial cytokines release (TSLP, IL-25, and IL-33). We investigated the release of TSLP, IL-25, and IL-33 by epithelial CRSwNP cells compared to epithelial sinus mucosa cells of patients with chronic rhinosinusitis without nasal polyps (CRSsNP). Methods. IL-25, IL-33, and TSLP were measured by ELISA in the supernatant of cell cultures derived by CRSwNP (9 patients, 6 atopic) and CRSsNP (7 patients, 2 atopic) in baseline condition and following stimulation with Dermatophagoides pteronyssinus (DP), Aspergillus fumigatus (AF), and poly(I:C). Results. CRSwNP epithelial cells released increased levels of IL-25 (from 0.12 ± 0.06 pg/ml to 0.27 ± 0.1 pg/ml, p<0.01) and TSLP (from 0.77 ± 0.5 pg/ml to 2.53 ± 1.17 pg/ml, p<0.001) following poly(I:C) stimulation, while CRSsNP epithelial cells released increased levels of IL-25 and IL-33 following AF and DP stimulation, respectively (IL-25: from 0.18 ± 0.07 pg/ml to 0.51 ± 0.1 pg/ml, p<0.001; IL-33: from 2.57 ± 1.3 pg/ml to 5.7 ± 3.1 pg/ml, p<0.001). Conclusions. CRSwNP epithelial cells release TSLP and IL-25 when stimulated by poly(I:C) but not by DP or AF, suggesting that viral infection may contribute to maintain and amplify the T2 immune response seen in CRSwNP.http://dx.doi.org/10.1155/2016/2643297 |
| spellingShingle | Monica Boita Caterina Bucca Giuseppe Riva Enrico Heffler Giovanni Rolla Release of Type 2 Cytokines by Epithelial Cells of Nasal Polyps Journal of Immunology Research |
| title | Release of Type 2 Cytokines by Epithelial Cells of Nasal Polyps |
| title_full | Release of Type 2 Cytokines by Epithelial Cells of Nasal Polyps |
| title_fullStr | Release of Type 2 Cytokines by Epithelial Cells of Nasal Polyps |
| title_full_unstemmed | Release of Type 2 Cytokines by Epithelial Cells of Nasal Polyps |
| title_short | Release of Type 2 Cytokines by Epithelial Cells of Nasal Polyps |
| title_sort | release of type 2 cytokines by epithelial cells of nasal polyps |
| url | http://dx.doi.org/10.1155/2016/2643297 |
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