AMPEROMETRIC MICROBIOSENSOR FOR THE DETERMINATION OF CHOLINE
The construction and performance of an amperometric microbiosensor for the determination of choline chloride by immobilizing choline oxidase on a cellulose acetate membrane are described. The procedure of choline determination involved the electrochemical detection of enzymatically generated hydroge...
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| Format: | Article |
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| Language: | English |
| Published: |
University of Tehran
1998-03-01
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| Series: | Journal of Sciences, Islamic Republic of Iran |
| Online Access: | https://jsciences.ut.ac.ir/article_31358_3e5df5649acee20c148141d101bedb5e.pdf |
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| Summary: | The construction and performance of an amperometric microbiosensor for the
determination of choline chloride by immobilizing choline oxidase on a cellulose
acetate membrane are described. The procedure of choline determination involved
the electrochemical detection of enzymatically generated hydrogen peroxide. This
microsensor has a linear response between 0.038-0.1 mM choline chloride. The
response time is 1-2 minutes. The optimal working conditions for the microsensor
were 0.1 M TRIS-HCl, pH = 8 at 30°C |
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| ISSN: | 1016-1104 2345-6914 |