Epidemiological and molecular characteristics of extraintestinal pathogenic escherichia coli isolated from diseased cattle and sheep in Xinjiang, China from 2015 to 2019
Abstract Escherichia coli has become a common causative agent of infections in animals, inflicting serious economic losses on livestock production and posing a threat to public health. Escherichia coli infection is common and tends to be complex in Xinjiang, a major region of cattle and sheep breedi...
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2025-01-01
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| Online Access: | https://doi.org/10.1186/s12917-025-04502-8 |
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| author | Xiaoxiao Gu Qin Wu Yingjin Chai Xin Huang Xia Zhou Mengli Han Tongzhong Wu Xingxing Zhang Fagang Zhong |
| author_facet | Xiaoxiao Gu Qin Wu Yingjin Chai Xin Huang Xia Zhou Mengli Han Tongzhong Wu Xingxing Zhang Fagang Zhong |
| author_sort | Xiaoxiao Gu |
| collection | DOAJ |
| description | Abstract Escherichia coli has become a common causative agent of infections in animals, inflicting serious economic losses on livestock production and posing a threat to public health. Escherichia coli infection is common and tends to be complex in Xinjiang, a major region of cattle and sheep breeding in China. This study aims to explore the current status and molecular characteristics of Escherichia coli infection in cattle and sheep in Xinjiang, as part of the disease prevention and control strategy. Herein we isolated Extraintestinal pathogenic Escherichia coli (ExPEC) from the liver, spleen, lung, heart, and lymph nodes of infected cattle and sheep (Xinjiang, China), and phylogenetic grouping, serotyping, and multilocus sequence typing were performed to determine epidemic and molecular characteristics. We also assessed their biofilm formation ability. A total of 132 strains of ExPEC were identified from diseased cattle and sheep, belonging to 7 phylogenetic groups. A and B1 are advantageous groups. Further, 22 serogroups were found, with O101 (26/132), O154 (14/132), and O65 (8/132) being the predominant ones. Among the seven sequence types identified by multilocus sequence typing, ST10 was the most common, followed by ST23 and ST457. Of 132, 105 (79.5%) strains were able to form biofilms: 15 strains (11.4%) were strong, 28 (21.2%) were medium, and 62 (47%) were weak biofilm producers. These findings will contribute to a better understanding of the molecular epidemiology of ExPEC in Xinjiang, China, and can be applied to the development, prevention, and disease control of future diagnostic tools and vaccine. |
| format | Article |
| id | doaj-art-8a46a07521054eecb2a99d1286365364 |
| institution | Kabale University |
| issn | 1746-6148 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
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| series | BMC Veterinary Research |
| spelling | doaj-art-8a46a07521054eecb2a99d12863653642025-02-02T12:29:14ZengBMCBMC Veterinary Research1746-61482025-01-012111910.1186/s12917-025-04502-8Epidemiological and molecular characteristics of extraintestinal pathogenic escherichia coli isolated from diseased cattle and sheep in Xinjiang, China from 2015 to 2019Xiaoxiao Gu0Qin Wu1Yingjin Chai2Xin Huang3Xia Zhou4Mengli Han5Tongzhong Wu6Xingxing Zhang7Fagang Zhong8College of Animal Science and Technology, Shihezi UniversityCollege of Animal Science and Technology, Shihezi UniversityCollege of Animal Science and Technology, Shihezi UniversityState Key Laboratory for Sheep Genetic Improvement and Healthy Production, Xinjiang Academy of Agricultural and Reclamation ScienceCollege of Animal Science and Technology, Shihezi UniversityState Key Laboratory for Sheep Genetic Improvement and Healthy Production, Xinjiang Academy of Agricultural and Reclamation ScienceState Key Laboratory for Sheep Genetic Improvement and Healthy Production, Xinjiang Academy of Agricultural and Reclamation ScienceState Key Laboratory for Sheep Genetic Improvement and Healthy Production, Xinjiang Academy of Agricultural and Reclamation ScienceState Key Laboratory for Sheep Genetic Improvement and Healthy Production, Xinjiang Academy of Agricultural and Reclamation ScienceAbstract Escherichia coli has become a common causative agent of infections in animals, inflicting serious economic losses on livestock production and posing a threat to public health. Escherichia coli infection is common and tends to be complex in Xinjiang, a major region of cattle and sheep breeding in China. This study aims to explore the current status and molecular characteristics of Escherichia coli infection in cattle and sheep in Xinjiang, as part of the disease prevention and control strategy. Herein we isolated Extraintestinal pathogenic Escherichia coli (ExPEC) from the liver, spleen, lung, heart, and lymph nodes of infected cattle and sheep (Xinjiang, China), and phylogenetic grouping, serotyping, and multilocus sequence typing were performed to determine epidemic and molecular characteristics. We also assessed their biofilm formation ability. A total of 132 strains of ExPEC were identified from diseased cattle and sheep, belonging to 7 phylogenetic groups. A and B1 are advantageous groups. Further, 22 serogroups were found, with O101 (26/132), O154 (14/132), and O65 (8/132) being the predominant ones. Among the seven sequence types identified by multilocus sequence typing, ST10 was the most common, followed by ST23 and ST457. Of 132, 105 (79.5%) strains were able to form biofilms: 15 strains (11.4%) were strong, 28 (21.2%) were medium, and 62 (47%) were weak biofilm producers. These findings will contribute to a better understanding of the molecular epidemiology of ExPEC in Xinjiang, China, and can be applied to the development, prevention, and disease control of future diagnostic tools and vaccine.https://doi.org/10.1186/s12917-025-04502-8ExPECPhylogenetic groupingSerotypeMultilocus sequence typingBiofilm |
| spellingShingle | Xiaoxiao Gu Qin Wu Yingjin Chai Xin Huang Xia Zhou Mengli Han Tongzhong Wu Xingxing Zhang Fagang Zhong Epidemiological and molecular characteristics of extraintestinal pathogenic escherichia coli isolated from diseased cattle and sheep in Xinjiang, China from 2015 to 2019 BMC Veterinary Research ExPEC Phylogenetic grouping Serotype Multilocus sequence typing Biofilm |
| title | Epidemiological and molecular characteristics of extraintestinal pathogenic escherichia coli isolated from diseased cattle and sheep in Xinjiang, China from 2015 to 2019 |
| title_full | Epidemiological and molecular characteristics of extraintestinal pathogenic escherichia coli isolated from diseased cattle and sheep in Xinjiang, China from 2015 to 2019 |
| title_fullStr | Epidemiological and molecular characteristics of extraintestinal pathogenic escherichia coli isolated from diseased cattle and sheep in Xinjiang, China from 2015 to 2019 |
| title_full_unstemmed | Epidemiological and molecular characteristics of extraintestinal pathogenic escherichia coli isolated from diseased cattle and sheep in Xinjiang, China from 2015 to 2019 |
| title_short | Epidemiological and molecular characteristics of extraintestinal pathogenic escherichia coli isolated from diseased cattle and sheep in Xinjiang, China from 2015 to 2019 |
| title_sort | epidemiological and molecular characteristics of extraintestinal pathogenic escherichia coli isolated from diseased cattle and sheep in xinjiang china from 2015 to 2019 |
| topic | ExPEC Phylogenetic grouping Serotype Multilocus sequence typing Biofilm |
| url | https://doi.org/10.1186/s12917-025-04502-8 |
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