IDENTIFICATION OF STEM RUST RESISTANCE SOURCES IN WHEAT BY USING MOLECULAR MARKERS

Puccinia graminis f. sp. tritici induces stem rust, which is the cause of a considerable crop loss. Race Ug99 of P. graminis f. sp. tritici (pathotype TTKS) is virulent to the majority of wheat varieties. According to FAO reports, major wheat-producing countries to the east of Iran (Afghanistan, Ind...

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Main Authors: A. M. Kokhmetova, M. N. Atishova
Format: Article
Language:English
Published: Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders 2014-12-01
Series:Вавиловский журнал генетики и селекции
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Online Access:https://vavilov.elpub.ru/jour/article/view/32
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author A. M. Kokhmetova
M. N. Atishova
author_facet A. M. Kokhmetova
M. N. Atishova
author_sort A. M. Kokhmetova
collection DOAJ
description Puccinia graminis f. sp. tritici induces stem rust, which is the cause of a considerable crop loss. Race Ug99 of P. graminis f. sp. tritici (pathotype TTKS) is virulent to the majority of wheat varieties. According to FAO reports, major wheat-producing countries to the east of Iran (Afghanistan, India, Pakistan, Turkmenistan, Uzbekistan and Kazakhstan) are greatly endangered. It is necessary to seek resistance sources to stem rust with identified Sr genes. Screening with PCR SSR and STS markers associated with effective Sr genes (Sr2, Sr22, Sr24 and Sr46) was undertaken to identify Sr gene sources. Twelve accessions with the Sr2-gene were identified. Seven resistant lines were found to possess Sr24/Lr24 genes. Sr46 was identified in one line. PCR analysis revealed the presence of Sr22 in six promising lines. The results are used in wheat breeding programs for stem rust resistance with marker-assisted selection.
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institution Kabale University
issn 2500-3259
language English
publishDate 2014-12-01
publisher Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
record_format Article
series Вавиловский журнал генетики и селекции
spelling doaj-art-8a3802bf486c4a0e9cb19db50c2897ee2025-02-01T09:57:58ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592014-12-0116113214116IDENTIFICATION OF STEM RUST RESISTANCE SOURCES IN WHEAT BY USING MOLECULAR MARKERSA. M. Kokhmetova0M. N. Atishova1Institute of Plant Biology and Biotechnology, Almaty, KazakhstanInstitute of Plant Biology and Biotechnology, Almaty, Kazakhstan,Puccinia graminis f. sp. tritici induces stem rust, which is the cause of a considerable crop loss. Race Ug99 of P. graminis f. sp. tritici (pathotype TTKS) is virulent to the majority of wheat varieties. According to FAO reports, major wheat-producing countries to the east of Iran (Afghanistan, India, Pakistan, Turkmenistan, Uzbekistan and Kazakhstan) are greatly endangered. It is necessary to seek resistance sources to stem rust with identified Sr genes. Screening with PCR SSR and STS markers associated with effective Sr genes (Sr2, Sr22, Sr24 and Sr46) was undertaken to identify Sr gene sources. Twelve accessions with the Sr2-gene were identified. Seven resistant lines were found to possess Sr24/Lr24 genes. Sr46 was identified in one line. PCR analysis revealed the presence of Sr22 in six promising lines. The results are used in wheat breeding programs for stem rust resistance with marker-assisted selection.https://vavilov.elpub.ru/jour/article/view/32wheatresistance genesstem rustmolecular markers
spellingShingle A. M. Kokhmetova
M. N. Atishova
IDENTIFICATION OF STEM RUST RESISTANCE SOURCES IN WHEAT BY USING MOLECULAR MARKERS
Вавиловский журнал генетики и селекции
wheat
resistance genes
stem rust
molecular markers
title IDENTIFICATION OF STEM RUST RESISTANCE SOURCES IN WHEAT BY USING MOLECULAR MARKERS
title_full IDENTIFICATION OF STEM RUST RESISTANCE SOURCES IN WHEAT BY USING MOLECULAR MARKERS
title_fullStr IDENTIFICATION OF STEM RUST RESISTANCE SOURCES IN WHEAT BY USING MOLECULAR MARKERS
title_full_unstemmed IDENTIFICATION OF STEM RUST RESISTANCE SOURCES IN WHEAT BY USING MOLECULAR MARKERS
title_short IDENTIFICATION OF STEM RUST RESISTANCE SOURCES IN WHEAT BY USING MOLECULAR MARKERS
title_sort identification of stem rust resistance sources in wheat by using molecular markers
topic wheat
resistance genes
stem rust
molecular markers
url https://vavilov.elpub.ru/jour/article/view/32
work_keys_str_mv AT amkokhmetova identificationofstemrustresistancesourcesinwheatbyusingmolecularmarkers
AT mnatishova identificationofstemrustresistancesourcesinwheatbyusingmolecularmarkers