In-Cell Assembly of scFv from Human Thyroid- Infiltrating B Cells

The construction of a large library of single-chain Fv (scFv) antibody fragments involves a random assortment of heavy and light chains. Although useful for the production of recombinant antibodies, this method is not adapted to the study of the autoantibody repertoire formed in vivo during autoimmu...

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Bibliographic Details
Main Authors: N. Chapal, M. Bouanani, M.J. Embleton, I. Navarro-Teulon, M. Biard-Piechaczyk, B. Pau, S. Peraldi-Roux
Format: Article
Language:English
Published: Taylor & Francis Group 1997-09-01
Series:BioTechniques
Online Access:https://www.future-science.com/doi/10.2144/97233rr03
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