PCR-mediated repeated chromosome splitting in Saccharomyces cerevisiae

Chromosome engineering is playing an increasingly important role in the functional analysis of genomes. A simple and efficient technology for manipulating large chromosomal segments is key to advancing these analyses. Here we describe a simple but innovative method to split chromosomes in Saccharomy...

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Bibliographic Details
Main Authors: Minetaka Sugiyama, Shigehito Ikushima, Toshimasa Nakazawa, Yoshinobu Kaneko, Satoshi Harashima
Format: Article
Language:English
Published: Taylor & Francis Group 2005-06-01
Series:BioTechniques
Online Access:https://www.future-science.com/doi/10.2144/05386RR01
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