PCR-mediated repeated chromosome splitting in Saccharomyces cerevisiae
Chromosome engineering is playing an increasingly important role in the functional analysis of genomes. A simple and efficient technology for manipulating large chromosomal segments is key to advancing these analyses. Here we describe a simple but innovative method to split chromosomes in Saccharomy...
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| Main Authors: | Minetaka Sugiyama, Shigehito Ikushima, Toshimasa Nakazawa, Yoshinobu Kaneko, Satoshi Harashima |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2005-06-01
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| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/05386RR01 |
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