Human Bone Marrow Contains Mesenchymal Stromal Stem Cells That Differentiate In Vitro into Contractile Myofibroblasts Controlling T Lymphocyte Proliferation
Mesenchymal stromal stem cells (MSC) that reside in the bone marrow (BM) can be amplified in vitro. In 2-dimension (D) cultures, MSC exhibit a morphology similar to fibroblasts, are able to inhibit T lymphocyte and natural killer cell proliferation, and can be differentiated into adipocytes, chondro...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2018/6134787 |
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| author | Yves Lecarpentier Olivier Schussler Antonija Sakic José Maria Rincon-Garriz Priscilla Soulie Marie-Luce Bochaton-Piallat Vincent Kindler |
| author_facet | Yves Lecarpentier Olivier Schussler Antonija Sakic José Maria Rincon-Garriz Priscilla Soulie Marie-Luce Bochaton-Piallat Vincent Kindler |
| author_sort | Yves Lecarpentier |
| collection | DOAJ |
| description | Mesenchymal stromal stem cells (MSC) that reside in the bone marrow (BM) can be amplified in vitro. In 2-dimension (D) cultures, MSC exhibit a morphology similar to fibroblasts, are able to inhibit T lymphocyte and natural killer cell proliferation, and can be differentiated into adipocytes, chondrocytes, or osteoblasts if exposed to specific media. Here we show that medullar MSC cultured in 2D formed an adherent stroma of cells expressing well-organized microfilaments containing α-smooth muscle actin and nonmuscle myosin heavy chain IIA. MSC could be grown in 3D in collagen membranes generating a structure which, upon exposition to 50 mM KCl or to an alternating electric current, developed a contractile strength that averaged 34 and 45 μN/mm2, respectively. Such mechanical tension was similar in intensity and in duration to that of human placenta and was annihilated by isosorbide dinitrate or 2,3-butanedione monoxime. Membranes devoid of MSC did not exhibit a significant contractility. Moreover, MSC nested in collagen membranes were able to control T lymphocyte proliferation, and differentiated into adipocytes, chondrocytes, or osteoblasts. Our observations show that BM-derived MSC cultured in collagen membranes spontaneously differentiate into contractile myofibroblasts exhibiting unexpected properties in terms of cell differentiation potential and of immunomodulatory function. |
| format | Article |
| id | doaj-art-8a131a68a4624261922969a104c70c63 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-8a131a68a4624261922969a104c70c632025-02-03T01:23:20ZengWileyStem Cells International1687-966X1687-96782018-01-01201810.1155/2018/61347876134787Human Bone Marrow Contains Mesenchymal Stromal Stem Cells That Differentiate In Vitro into Contractile Myofibroblasts Controlling T Lymphocyte ProliferationYves Lecarpentier0Olivier Schussler1Antonija Sakic2José Maria Rincon-Garriz3Priscilla Soulie4Marie-Luce Bochaton-Piallat5Vincent Kindler6Centre de Recherche Clinique, Grand Hôpital de l’Est Francillien, 77104 Meaux, FranceDepartment of Cardiovascular Surgery, Research Laboratory, University Hospitals, Faculty of Medicine, Geneva, SwitzerlandDepartment of Pathology and Immunology, Centre Médical Universitaire Geneva Faculty of Medicine, Geneva, SwitzerlandDepartment of Specialties in Medicine, Hematology Service, Geneva University Hospitals, Faculty of Medicine, Geneva, SwitzerlandDepartment of Histology, Centre Médical Universitaire Geneva Faculty of Medicine, Geneva, SwitzerlandDepartment of Pathology and Immunology, Centre Médical Universitaire Geneva Faculty of Medicine, Geneva, SwitzerlandDepartment of Specialties in Medicine, Hematology Service, Geneva University Hospitals, Faculty of Medicine, Geneva, SwitzerlandMesenchymal stromal stem cells (MSC) that reside in the bone marrow (BM) can be amplified in vitro. In 2-dimension (D) cultures, MSC exhibit a morphology similar to fibroblasts, are able to inhibit T lymphocyte and natural killer cell proliferation, and can be differentiated into adipocytes, chondrocytes, or osteoblasts if exposed to specific media. Here we show that medullar MSC cultured in 2D formed an adherent stroma of cells expressing well-organized microfilaments containing α-smooth muscle actin and nonmuscle myosin heavy chain IIA. MSC could be grown in 3D in collagen membranes generating a structure which, upon exposition to 50 mM KCl or to an alternating electric current, developed a contractile strength that averaged 34 and 45 μN/mm2, respectively. Such mechanical tension was similar in intensity and in duration to that of human placenta and was annihilated by isosorbide dinitrate or 2,3-butanedione monoxime. Membranes devoid of MSC did not exhibit a significant contractility. Moreover, MSC nested in collagen membranes were able to control T lymphocyte proliferation, and differentiated into adipocytes, chondrocytes, or osteoblasts. Our observations show that BM-derived MSC cultured in collagen membranes spontaneously differentiate into contractile myofibroblasts exhibiting unexpected properties in terms of cell differentiation potential and of immunomodulatory function.http://dx.doi.org/10.1155/2018/6134787 |
| spellingShingle | Yves Lecarpentier Olivier Schussler Antonija Sakic José Maria Rincon-Garriz Priscilla Soulie Marie-Luce Bochaton-Piallat Vincent Kindler Human Bone Marrow Contains Mesenchymal Stromal Stem Cells That Differentiate In Vitro into Contractile Myofibroblasts Controlling T Lymphocyte Proliferation Stem Cells International |
| title | Human Bone Marrow Contains Mesenchymal Stromal Stem Cells That Differentiate In Vitro into Contractile Myofibroblasts Controlling T Lymphocyte Proliferation |
| title_full | Human Bone Marrow Contains Mesenchymal Stromal Stem Cells That Differentiate In Vitro into Contractile Myofibroblasts Controlling T Lymphocyte Proliferation |
| title_fullStr | Human Bone Marrow Contains Mesenchymal Stromal Stem Cells That Differentiate In Vitro into Contractile Myofibroblasts Controlling T Lymphocyte Proliferation |
| title_full_unstemmed | Human Bone Marrow Contains Mesenchymal Stromal Stem Cells That Differentiate In Vitro into Contractile Myofibroblasts Controlling T Lymphocyte Proliferation |
| title_short | Human Bone Marrow Contains Mesenchymal Stromal Stem Cells That Differentiate In Vitro into Contractile Myofibroblasts Controlling T Lymphocyte Proliferation |
| title_sort | human bone marrow contains mesenchymal stromal stem cells that differentiate in vitro into contractile myofibroblasts controlling t lymphocyte proliferation |
| url | http://dx.doi.org/10.1155/2018/6134787 |
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