Epichloë scottii sp. nov., a new endophyte isolated from Melica uniflora is the missing ancestor of Epichloë disjuncta
Abstract Here we describe a new, haploid and stroma forming species within the genus Epichloë, as Epichloë scottii sp. nov. The fungus was isolated from Melica uniflora growing in Bad Harzburg, Germany. Phylogenetic reconstruction using a combined dataset of the tubB and tefA genes strongly support...
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| Language: | English |
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BMC
2022-02-01
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| Series: | IMA Fungus |
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| Online Access: | https://doi.org/10.1186/s43008-022-00088-0 |
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| author | Torsten Thünen Yvonne Becker Murray P. Cox Samad Ashrafi |
| author_facet | Torsten Thünen Yvonne Becker Murray P. Cox Samad Ashrafi |
| author_sort | Torsten Thünen |
| collection | DOAJ |
| description | Abstract Here we describe a new, haploid and stroma forming species within the genus Epichloë, as Epichloë scottii sp. nov. The fungus was isolated from Melica uniflora growing in Bad Harzburg, Germany. Phylogenetic reconstruction using a combined dataset of the tubB and tefA genes strongly support that E. scottii is a distinct species and the so far unknown ancestor species of the hybrid E. disjuncta. A distribution analysis showed a high infection rate in close vicinity of the initial sampling site and only two more spots with low infection rates. Genetic variations in key genes required for alkaloid production suggested that E. scottii sp. nov. might not be capable of producing any of the major alkaloids including ergot alkaloid, loline, indole-diterpene and peramine. All isolates and individuals found in the distribution analysis were identified as mating-type B explaining the lack of mature stromata during this study. We further release a telomere-to-telomere de novo assembly of all seven chromosomes and the mitogenome of E. scottii sp. nov. |
| format | Article |
| id | doaj-art-89e3208739e6447e86509f55a5ef0be1 |
| institution | Kabale University |
| issn | 2210-6359 |
| language | English |
| publishDate | 2022-02-01 |
| publisher | BMC |
| record_format | Article |
| series | IMA Fungus |
| spelling | doaj-art-89e3208739e6447e86509f55a5ef0be12025-02-02T19:28:42ZengBMCIMA Fungus2210-63592022-02-0113111810.1186/s43008-022-00088-0Epichloë scottii sp. nov., a new endophyte isolated from Melica uniflora is the missing ancestor of Epichloë disjunctaTorsten Thünen0Yvonne Becker1Murray P. Cox2Samad Ashrafi3Institute for Crop and Soil Science, Julius Kühn Institute (JKI) – Federal Research Centre for Cultivated PlantsInstitute for Epidemiology and Pathogen Diagnostics, Julius Kühn Institute (JKI) – Federal Research Centre for Cultivated PlantsStatistics and Bioinformatics Group, School of Fundamental Sciences, Massey UniversityInstitute for Epidemiology and Pathogen Diagnostics, Julius Kühn Institute (JKI) – Federal Research Centre for Cultivated PlantsAbstract Here we describe a new, haploid and stroma forming species within the genus Epichloë, as Epichloë scottii sp. nov. The fungus was isolated from Melica uniflora growing in Bad Harzburg, Germany. Phylogenetic reconstruction using a combined dataset of the tubB and tefA genes strongly support that E. scottii is a distinct species and the so far unknown ancestor species of the hybrid E. disjuncta. A distribution analysis showed a high infection rate in close vicinity of the initial sampling site and only two more spots with low infection rates. Genetic variations in key genes required for alkaloid production suggested that E. scottii sp. nov. might not be capable of producing any of the major alkaloids including ergot alkaloid, loline, indole-diterpene and peramine. All isolates and individuals found in the distribution analysis were identified as mating-type B explaining the lack of mature stromata during this study. We further release a telomere-to-telomere de novo assembly of all seven chromosomes and the mitogenome of E. scottii sp. nov.https://doi.org/10.1186/s43008-022-00088-0EpichloëMelica unifloraNew speciesAlkaloid profileBUSCO multigene-phylogenyOxford nanopore |
| spellingShingle | Torsten Thünen Yvonne Becker Murray P. Cox Samad Ashrafi Epichloë scottii sp. nov., a new endophyte isolated from Melica uniflora is the missing ancestor of Epichloë disjuncta IMA Fungus Epichloë Melica uniflora New species Alkaloid profile BUSCO multigene-phylogeny Oxford nanopore |
| title | Epichloë scottii sp. nov., a new endophyte isolated from Melica uniflora is the missing ancestor of Epichloë disjuncta |
| title_full | Epichloë scottii sp. nov., a new endophyte isolated from Melica uniflora is the missing ancestor of Epichloë disjuncta |
| title_fullStr | Epichloë scottii sp. nov., a new endophyte isolated from Melica uniflora is the missing ancestor of Epichloë disjuncta |
| title_full_unstemmed | Epichloë scottii sp. nov., a new endophyte isolated from Melica uniflora is the missing ancestor of Epichloë disjuncta |
| title_short | Epichloë scottii sp. nov., a new endophyte isolated from Melica uniflora is the missing ancestor of Epichloë disjuncta |
| title_sort | epichloe scottii sp nov a new endophyte isolated from melica uniflora is the missing ancestor of epichloe disjuncta |
| topic | Epichloë Melica uniflora New species Alkaloid profile BUSCO multigene-phylogeny Oxford nanopore |
| url | https://doi.org/10.1186/s43008-022-00088-0 |
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