Evaluation of immunomodulatory effects of lamotrigine in BALB/c mice
Modulation of the immune system has recently been shown to be involved in the pharmacological effects of old antiepileptic drugs and in the pathogenesis of epilepsy. Therefore, the most recent guidelines for immunotoxicological evaluation of drugs were consulted to investigate the immunomodulatory e...
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| Format: | Article |
| Language: | English |
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Sciendo
2017-12-01
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| Series: | Acta Pharmaceutica |
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| Online Access: | https://doi.org/10.1515/acph-2017-0035 |
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| author | Abu-Rish Eman Y. Elhayek Shada Y. Mohamed Yehia S. Hamad Islam Bustanji Yasser |
| author_facet | Abu-Rish Eman Y. Elhayek Shada Y. Mohamed Yehia S. Hamad Islam Bustanji Yasser |
| author_sort | Abu-Rish Eman Y. |
| collection | DOAJ |
| description | Modulation of the immune system has recently been shown to be involved in the pharmacological effects of old antiepileptic drugs and in the pathogenesis of epilepsy. Therefore, the most recent guidelines for immunotoxicological evaluation of drugs were consulted to investigate the immunomodulatory effects of lamotrigine, a newer antiepileptic drug, in BALB/c mice. These included the in vivo effects of lamotrigine on delayed-type hypersensitivity (DTH) response to sheep red blood cell (SRBC) antigens, hemagglutination titer assays and hematological changes. In vitro effects of lamotrigine on ConA-induced splenocyte proliferation and cytokine secretion were assessed. The results showed that lamotrigine treatment significantly increased the DTH response to SRBC in the mouse model of this study. This was accompanied by a significant increase in relative monocyte and neutrophil counts and in spleen cellularity. Lamotrigine significantly inhibited ConA-induced splenocyte proliferation in vitro and it significantly inhibited IL-2 and TNF-α secretion in ConA-stimulated splenocytes. In conclusion, the results demonstrated significant immunomodulatory effects of lamotrigine in BALB/c mice. These data could expand the understanding of lamotrigine-induced adverse reactions and its role in modulating the immune system in epilepsy. |
| format | Article |
| id | doaj-art-89e31b77bed448a89a20d290de44893d |
| institution | Kabale University |
| issn | 1846-9558 |
| language | English |
| publishDate | 2017-12-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Pharmaceutica |
| spelling | doaj-art-89e31b77bed448a89a20d290de44893d2025-02-02T10:06:42ZengSciendoActa Pharmaceutica1846-95582017-12-0167454355510.1515/acph-2017-0035acph-2017-0035Evaluation of immunomodulatory effects of lamotrigine in BALB/c miceAbu-Rish Eman Y.0Elhayek Shada Y.1Mohamed Yehia S.2Hamad Islam3Bustanji Yasser4Department of Biopharmaceutics and Clinical Pharmacy, School of Pharmacy The University of Jordan, Amman 11942, JordanDepartment of Biopharmaceutics and Clinical Pharmacy, School of Pharmacy The University of Jordan, Amman 11942, JordanDepartment of Medical Microbiology College of Medicine, University of Dammam, Dammam 31451 PO Box 2114, Saudi ArabiaDepartment of Pharmacy American University of Madaba PO Box 2882, Amman 11821, JordanDepartment of Biopharmaceutics and Clinical Pharmacy, School of Pharmacy The University of Jordan, Amman 11942, JordanModulation of the immune system has recently been shown to be involved in the pharmacological effects of old antiepileptic drugs and in the pathogenesis of epilepsy. Therefore, the most recent guidelines for immunotoxicological evaluation of drugs were consulted to investigate the immunomodulatory effects of lamotrigine, a newer antiepileptic drug, in BALB/c mice. These included the in vivo effects of lamotrigine on delayed-type hypersensitivity (DTH) response to sheep red blood cell (SRBC) antigens, hemagglutination titer assays and hematological changes. In vitro effects of lamotrigine on ConA-induced splenocyte proliferation and cytokine secretion were assessed. The results showed that lamotrigine treatment significantly increased the DTH response to SRBC in the mouse model of this study. This was accompanied by a significant increase in relative monocyte and neutrophil counts and in spleen cellularity. Lamotrigine significantly inhibited ConA-induced splenocyte proliferation in vitro and it significantly inhibited IL-2 and TNF-α secretion in ConA-stimulated splenocytes. In conclusion, the results demonstrated significant immunomodulatory effects of lamotrigine in BALB/c mice. These data could expand the understanding of lamotrigine-induced adverse reactions and its role in modulating the immune system in epilepsy.https://doi.org/10.1515/acph-2017-0035lamotrigineepilepsycytokinehypersensitivityimmunomodulatory effectimmunotoxicity |
| spellingShingle | Abu-Rish Eman Y. Elhayek Shada Y. Mohamed Yehia S. Hamad Islam Bustanji Yasser Evaluation of immunomodulatory effects of lamotrigine in BALB/c mice Acta Pharmaceutica lamotrigine epilepsy cytokine hypersensitivity immunomodulatory effect immunotoxicity |
| title | Evaluation of immunomodulatory effects of lamotrigine in BALB/c mice |
| title_full | Evaluation of immunomodulatory effects of lamotrigine in BALB/c mice |
| title_fullStr | Evaluation of immunomodulatory effects of lamotrigine in BALB/c mice |
| title_full_unstemmed | Evaluation of immunomodulatory effects of lamotrigine in BALB/c mice |
| title_short | Evaluation of immunomodulatory effects of lamotrigine in BALB/c mice |
| title_sort | evaluation of immunomodulatory effects of lamotrigine in balb c mice |
| topic | lamotrigine epilepsy cytokine hypersensitivity immunomodulatory effect immunotoxicity |
| url | https://doi.org/10.1515/acph-2017-0035 |
| work_keys_str_mv | AT aburishemany evaluationofimmunomodulatoryeffectsoflamotrigineinbalbcmice AT elhayekshaday evaluationofimmunomodulatoryeffectsoflamotrigineinbalbcmice AT mohamedyehias evaluationofimmunomodulatoryeffectsoflamotrigineinbalbcmice AT hamadislam evaluationofimmunomodulatoryeffectsoflamotrigineinbalbcmice AT bustanjiyasser evaluationofimmunomodulatoryeffectsoflamotrigineinbalbcmice |