Apoptosis of colon cancer CT-26 cells induced polysaccharide from Cyclocarya paliurus and its phosphorylated derivative via intrinsic mitochondrial passway
In this study, the antitumor properties and the possible molecular mechanisms of Cyclocarya paliurus polysaccharide (CP) and its phosphorylated derivative (P-CP) on CT-26 mouse colon carcinoma cells were investigated. Results found that CP had high inhibition ratio against CT-26 cells. The flow cyto...
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Tsinghua University Press
2023-09-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453023000022 |
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| author | Liuming Xie Mingyue Shen Rong Huang Xuan Liu Yue Yu Hanyu Lu Jianhua Xie |
| author_facet | Liuming Xie Mingyue Shen Rong Huang Xuan Liu Yue Yu Hanyu Lu Jianhua Xie |
| author_sort | Liuming Xie |
| collection | DOAJ |
| description | In this study, the antitumor properties and the possible molecular mechanisms of Cyclocarya paliurus polysaccharide (CP) and its phosphorylated derivative (P-CP) on CT-26 mouse colon carcinoma cells were investigated. Results found that CP had high inhibition ratio against CT-26 cells. The flow cytometry results found that CP treatment could cause the intracellular acidification, arrest the cell cycle in the S phase and increase reactive oxygen species generation. Additionally, CP treatment triggered mitochondrial membrane potential depolarization and Ca2+ overloading, and broke down the balance of antioxidant system, Na+/K+-ATPase and Ca2+-ATPase. Further analysis found CP induced cell apoptosis through improving the activities of caspase-3 and caspase-9, and increasing the level of cytochrome C. Furthermore, the comparative study of antitumor effect on CT-26 cells displayed that the phosphorylation enhanced antitumor activities of polysaccharides. These results suggest CP is a potential natural therapeutic agent for colon cancer and phosphorylation represents an effective method of enhancing the antitumor activity of CP. |
| format | Article |
| id | doaj-art-89d3e85b4bd446c3923fd189d5789ccd |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2023-09-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-89d3e85b4bd446c3923fd189d5789ccd2025-02-03T05:13:48ZengTsinghua University PressFood Science and Human Wellness2213-45302023-09-0112515451556Apoptosis of colon cancer CT-26 cells induced polysaccharide from Cyclocarya paliurus and its phosphorylated derivative via intrinsic mitochondrial passwayLiuming Xie0Mingyue Shen1Rong Huang2Xuan Liu3Yue Yu4Hanyu Lu5Jianhua Xie6State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaCorresponding authors at: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China; State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaCorresponding authors at: State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China; State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaIn this study, the antitumor properties and the possible molecular mechanisms of Cyclocarya paliurus polysaccharide (CP) and its phosphorylated derivative (P-CP) on CT-26 mouse colon carcinoma cells were investigated. Results found that CP had high inhibition ratio against CT-26 cells. The flow cytometry results found that CP treatment could cause the intracellular acidification, arrest the cell cycle in the S phase and increase reactive oxygen species generation. Additionally, CP treatment triggered mitochondrial membrane potential depolarization and Ca2+ overloading, and broke down the balance of antioxidant system, Na+/K+-ATPase and Ca2+-ATPase. Further analysis found CP induced cell apoptosis through improving the activities of caspase-3 and caspase-9, and increasing the level of cytochrome C. Furthermore, the comparative study of antitumor effect on CT-26 cells displayed that the phosphorylation enhanced antitumor activities of polysaccharides. These results suggest CP is a potential natural therapeutic agent for colon cancer and phosphorylation represents an effective method of enhancing the antitumor activity of CP.http://www.sciencedirect.com/science/article/pii/S2213453023000022Cyclocarya paliurus polysaccharidePhosphorylatedColorectal cancerMitochondrial apoptosis pathway |
| spellingShingle | Liuming Xie Mingyue Shen Rong Huang Xuan Liu Yue Yu Hanyu Lu Jianhua Xie Apoptosis of colon cancer CT-26 cells induced polysaccharide from Cyclocarya paliurus and its phosphorylated derivative via intrinsic mitochondrial passway Food Science and Human Wellness Cyclocarya paliurus polysaccharide Phosphorylated Colorectal cancer Mitochondrial apoptosis pathway |
| title | Apoptosis of colon cancer CT-26 cells induced polysaccharide from Cyclocarya paliurus and its phosphorylated derivative via intrinsic mitochondrial passway |
| title_full | Apoptosis of colon cancer CT-26 cells induced polysaccharide from Cyclocarya paliurus and its phosphorylated derivative via intrinsic mitochondrial passway |
| title_fullStr | Apoptosis of colon cancer CT-26 cells induced polysaccharide from Cyclocarya paliurus and its phosphorylated derivative via intrinsic mitochondrial passway |
| title_full_unstemmed | Apoptosis of colon cancer CT-26 cells induced polysaccharide from Cyclocarya paliurus and its phosphorylated derivative via intrinsic mitochondrial passway |
| title_short | Apoptosis of colon cancer CT-26 cells induced polysaccharide from Cyclocarya paliurus and its phosphorylated derivative via intrinsic mitochondrial passway |
| title_sort | apoptosis of colon cancer ct 26 cells induced polysaccharide from cyclocarya paliurus and its phosphorylated derivative via intrinsic mitochondrial passway |
| topic | Cyclocarya paliurus polysaccharide Phosphorylated Colorectal cancer Mitochondrial apoptosis pathway |
| url | http://www.sciencedirect.com/science/article/pii/S2213453023000022 |
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