Production of citric acid from food waste using Aspergillus Tubingensis and Aspergillus Niger

Millions of tons of organic waste are produced annually, but no proper disposal method is practiced that minimizes organic waste in an environmentally friendly manner. Such waste has harmful effects on human health and the environment. Organic wastes can be utilized to synthesize industrial products...

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Main Authors: Aabida Parveen, Tooba Naveed, Suraiya Jabeen, Farman Ahmed, Nida Saleem
Format: Article
Language:English
Published: Iranian Research Organization for Science and Technology (IROST) 2025-04-01
Series:Advances in Environmental Technology
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Online Access:https://aet.irost.ir/article_1511_5960a764cc01d1c4d9dc559e816ab3f6.pdf
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author Aabida Parveen
Tooba Naveed
Suraiya Jabeen
Farman Ahmed
Nida Saleem
author_facet Aabida Parveen
Tooba Naveed
Suraiya Jabeen
Farman Ahmed
Nida Saleem
author_sort Aabida Parveen
collection DOAJ
description Millions of tons of organic waste are produced annually, but no proper disposal method is practiced that minimizes organic waste in an environmentally friendly manner. Such waste has harmful effects on human health and the environment. Organic wastes can be utilized to synthesize industrial products like citric acid. A number of microorganisms have been used in fermentation studies of citric acid. The genus Aspergillus is reported as being a prominent species in the production of a variety of enzymes and organic acids. Exploring more productive strains and valorization of biomass are the most crucial steps in promoting economically viable and useful industrial products such as citric acid, etc. Thus, the present work aimed to assess the potential of locally isolated A. tubingensis for the first time and A.niger for citric acid production. Segregated kitchen waste, crude molasses, and sucrose were used as substrates. Additionally, the effects of pH, carbon, nitrogen, and methanol were also monitored. The findings indicated that the highest yield, i.e., 16.14+0.03g/l, was produced by the A. tubingensisin fermentation media containing sucrose, 1.0% ammonium nitrate (AN), and 2.0% methanol, while 15.97+0.01g/l was produced by the A.nigerin fermentation media containing sucrose, 1.0% ammonium nitrate (AN), and 1.0 % methanol. The addition of methanol in the Aspergillus tubingens is fermentation media produced higher yields of citric acid. The findings demonstrated that A. tubingensis is a potential contender for the production of citric acid. Furthermore, kitchen waste could be a sustainable and cost-effective substrate for citric acid production and can be managed in an eco-friendly way.
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spelling doaj-art-89c6e0a26dfd4bc8809a22d5cdedbb442025-08-20T03:01:35ZengIranian Research Organization for Science and Technology (IROST)Advances in Environmental Technology2476-66742476-47792025-04-0111220721910.22104/aet.2025.7212.19891511Production of citric acid from food waste using Aspergillus Tubingensis and Aspergillus NigerAabida Parveen0Tooba Naveed1Suraiya Jabeen2Farman Ahmed3Nida Saleem4Institute of Environmental Studies, University of Karachi, Karachi, 75270, PakistanCentre for Environmental Studies, PCSIR Labs Complex, Shahra-e-SalimuzzanSiddiqui, Off University Road, Karachi, 75280, PakistanInstitute of Environmental Studies, University of Karachi, Karachi, 75270, PakistanFood and Feed Safety Laboratory, Food and Marine Resources Research Centre, PCSIR Laboratories Complex, Shahrah-e-SalimuzzamanSiddiqui, Off University Road, Karachi, 75280, Sindh, 74200, PakistanFood and Feed Safety Laboratory, Food and Marine Resources Research Centre, PCSIR Laboratories Complex, Shahrah-e-SalimuzzamanSiddiqui, Off University Road, Karachi, 75280, Sindh, 74200, PakistanMillions of tons of organic waste are produced annually, but no proper disposal method is practiced that minimizes organic waste in an environmentally friendly manner. Such waste has harmful effects on human health and the environment. Organic wastes can be utilized to synthesize industrial products like citric acid. A number of microorganisms have been used in fermentation studies of citric acid. The genus Aspergillus is reported as being a prominent species in the production of a variety of enzymes and organic acids. Exploring more productive strains and valorization of biomass are the most crucial steps in promoting economically viable and useful industrial products such as citric acid, etc. Thus, the present work aimed to assess the potential of locally isolated A. tubingensis for the first time and A.niger for citric acid production. Segregated kitchen waste, crude molasses, and sucrose were used as substrates. Additionally, the effects of pH, carbon, nitrogen, and methanol were also monitored. The findings indicated that the highest yield, i.e., 16.14+0.03g/l, was produced by the A. tubingensisin fermentation media containing sucrose, 1.0% ammonium nitrate (AN), and 2.0% methanol, while 15.97+0.01g/l was produced by the A.nigerin fermentation media containing sucrose, 1.0% ammonium nitrate (AN), and 1.0 % methanol. The addition of methanol in the Aspergillus tubingens is fermentation media produced higher yields of citric acid. The findings demonstrated that A. tubingensis is a potential contender for the production of citric acid. Furthermore, kitchen waste could be a sustainable and cost-effective substrate for citric acid production and can be managed in an eco-friendly way.https://aet.irost.ir/article_1511_5960a764cc01d1c4d9dc559e816ab3f6.pdfcitric acidwaste managementfermentationfood waste valorizationsustainable production
spellingShingle Aabida Parveen
Tooba Naveed
Suraiya Jabeen
Farman Ahmed
Nida Saleem
Production of citric acid from food waste using Aspergillus Tubingensis and Aspergillus Niger
Advances in Environmental Technology
citric acid
waste management
fermentation
food waste valorization
sustainable production
title Production of citric acid from food waste using Aspergillus Tubingensis and Aspergillus Niger
title_full Production of citric acid from food waste using Aspergillus Tubingensis and Aspergillus Niger
title_fullStr Production of citric acid from food waste using Aspergillus Tubingensis and Aspergillus Niger
title_full_unstemmed Production of citric acid from food waste using Aspergillus Tubingensis and Aspergillus Niger
title_short Production of citric acid from food waste using Aspergillus Tubingensis and Aspergillus Niger
title_sort production of citric acid from food waste using aspergillus tubingensis and aspergillus niger
topic citric acid
waste management
fermentation
food waste valorization
sustainable production
url https://aet.irost.ir/article_1511_5960a764cc01d1c4d9dc559e816ab3f6.pdf
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AT suraiyajabeen productionofcitricacidfromfoodwasteusingaspergillustubingensisandaspergillusniger
AT farmanahmed productionofcitricacidfromfoodwasteusingaspergillustubingensisandaspergillusniger
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