Development of a novel Ga-containing hydroxyapatite/chlorhexidine biomaterial with antibacterial properties for future application in bone tissue engineering: an experimental and theoretical study

Development of a novel Ga-containing hydroxyapatite/chlorhexidine biomaterial with antibacterial properties for future application in bone tissue engineering: an experimental and theoretical study

Abstract This study focuses on synthesizing gallium-containing hydroxyapatite (Ga-HA) with chlorhexidine (CLX) for potential use in bone and dental tissue restoration. The Ga-HA/CLX materials were prepared using a suspension-precipitation method and were surface-functionalized with varying CLX conce...

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Main Authors: Ewerton Gomes Vieira, Ricardo Barbosa Sousa, Marcos Pereira da Silva, Régis Casimiro Leal, Anderson Gomes Vieira, Wiury Chaves de Abreu, André L. Menezes de Oliveira, Maria Gardênnia da Fonseca, Santiago Medina Carrasco, Josy Anteveli Osajima, Edson C. Silva-Filho
Format: Article
Language:English
Published: Associação Brasileira de Cerâmica 2025-04-01
Series:Cerâmica
Subjects:
Antibacterial properties
Bone repair
Chlorhexidine-functionalized biomaterials
Density Functional Theory (DFT)
Surface functionalization
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0366-69132025000100309&lng=en&tlng=en
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Summary:Abstract This study focuses on synthesizing gallium-containing hydroxyapatite (Ga-HA) with chlorhexidine (CLX) for potential use in bone and dental tissue restoration. The Ga-HA/CLX materials were prepared using a suspension-precipitation method and were surface-functionalized with varying CLX concentrations. X-ray diffraction analysis confirmed the hexagonal structure of Ga-HA with space group P63/m, while XPS revealed the presence of gallium and a Ca/P ratio ranging from 1.50 to 1.72. Infrared spectra exhibited characteristic bands for phosphate and CH2 groups, indicating CLX incorporation. The Ga-HA/CLX materials demonstrated 100% inhibitory efficiency against Staphylococcus aureus and Escherichia coli bacterial strains. MTT assay indicated enhanced cell viability in the presence of gallium, with the Ga-HA/CLX-0.20 material classified as non-toxic with 81.0 ± 3% cell proliferation. Density Functional Theory calculations supported favorable thermodynamics in the interaction between hydroxyapatite and chlorhexidine. Overall, Ga-HA/CLX materials exhibit promising properties for biomedical applications.
ISSN:1678-4553

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