Dynamic non-coding RNA biomarker reveals lung injury and repair induced by polystyrene nanoplastics
Polystyrene nanoplastics (PS-NPs) are omnipresent in the air and can be inhaled by humans. However, their long-term adverse implications and toxicological mechanisms for human respiratory health are unclear. Therefore, this study aims to provide new insights into the pulmonary toxicity of PS-NPs usi...
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Elsevier
2025-01-01
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| Series: | Environment International |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0160412025000170 |
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| author | Sheng Yang Yiling Ge Tianyi Zhang Lihong Yin Yuepu Pu Zaozao Chen Geyu Liang |
| author_facet | Sheng Yang Yiling Ge Tianyi Zhang Lihong Yin Yuepu Pu Zaozao Chen Geyu Liang |
| author_sort | Sheng Yang |
| collection | DOAJ |
| description | Polystyrene nanoplastics (PS-NPs) are omnipresent in the air and can be inhaled by humans. However, their long-term adverse implications and toxicological mechanisms for human respiratory health are unclear. Therefore, this study aims to provide new insights into the pulmonary toxicity of PS-NPs using mice and organoid models. After subacute and subchronic inhalation of PS-NPs, mice showed pronounced lung injury characterized by respiratory rate changes, altered hematology, and histological evidence of tissue damage and oxidative stress. Similarly, repeated PS-NPs exposure also restricted organoid growth and cause oxidative damage. Notably, through BisqueRNA analysis for a single-cell dataset and canonical markers verification, it was found that PS-NPs induced the emergence and accumulation of transitional cells, suggesting impaired alveolar epithelial repair processes. Sequencing analyses revealed dynamic alterations in non-coding RNA (ncRNA) profiles, including circRNAs and lncRNAs, in response to PS-NPs exposure. Moreover, temporal profiling highlighted distinct sets of ncRNAs as early and progression-associated biomarkers of PS-NP-induced lung injury. These biomarkers correlated with aberrant transitional cells, implicating their roles in disrupted cellular differen tiation and repair mechanisms. Overall, this study observed the multifaceted toxicological responses of PS-NPs to the respiratory system, emphasizing the critical involvement of ncRNAs in mediating PS-NP-induce transitional cells, which was crucial for elucidating the pathophysiology of nanoplastic-induced lung injury and developing targeted therapeutic strategies. |
| format | Article |
| id | doaj-art-89a5a472370542f8acecf1fb6f12fda6 |
| institution | Kabale University |
| issn | 0160-4120 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Environment International |
| spelling | doaj-art-89a5a472370542f8acecf1fb6f12fda62025-01-24T04:44:17ZengElsevierEnvironment International0160-41202025-01-01195109266Dynamic non-coding RNA biomarker reveals lung injury and repair induced by polystyrene nanoplasticsSheng Yang0Yiling Ge1Tianyi Zhang2Lihong Yin3Yuepu Pu4Zaozao Chen5Geyu Liang6Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing, Jiangsu, 210009, China; Institute of Biomedical Devices (Suzhou), Southeast University, Suzhou, 215163, ChinaKey Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing, Jiangsu, 210009, China; Institute of Biomedical Devices (Suzhou), Southeast University, Suzhou, 215163, ChinaKey Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing, Jiangsu, 210009, China; Institute of Biomedical Devices (Suzhou), Southeast University, Suzhou, 215163, ChinaKey Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing, Jiangsu, 210009, ChinaKey Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing, Jiangsu, 210009, ChinaState Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, Jiangsu, 210096, China; Corresponding authors.Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Public Health, Southeast University, Nanjing, Jiangsu, 210009, China; Corresponding authors.Polystyrene nanoplastics (PS-NPs) are omnipresent in the air and can be inhaled by humans. However, their long-term adverse implications and toxicological mechanisms for human respiratory health are unclear. Therefore, this study aims to provide new insights into the pulmonary toxicity of PS-NPs using mice and organoid models. After subacute and subchronic inhalation of PS-NPs, mice showed pronounced lung injury characterized by respiratory rate changes, altered hematology, and histological evidence of tissue damage and oxidative stress. Similarly, repeated PS-NPs exposure also restricted organoid growth and cause oxidative damage. Notably, through BisqueRNA analysis for a single-cell dataset and canonical markers verification, it was found that PS-NPs induced the emergence and accumulation of transitional cells, suggesting impaired alveolar epithelial repair processes. Sequencing analyses revealed dynamic alterations in non-coding RNA (ncRNA) profiles, including circRNAs and lncRNAs, in response to PS-NPs exposure. Moreover, temporal profiling highlighted distinct sets of ncRNAs as early and progression-associated biomarkers of PS-NP-induced lung injury. These biomarkers correlated with aberrant transitional cells, implicating their roles in disrupted cellular differen tiation and repair mechanisms. Overall, this study observed the multifaceted toxicological responses of PS-NPs to the respiratory system, emphasizing the critical involvement of ncRNAs in mediating PS-NP-induce transitional cells, which was crucial for elucidating the pathophysiology of nanoplastic-induced lung injury and developing targeted therapeutic strategies.http://www.sciencedirect.com/science/article/pii/S0160412025000170Polystyrene nanoplasticsLung injuryTime-resolved analysisTransitional cellsnon-coding RNA |
| spellingShingle | Sheng Yang Yiling Ge Tianyi Zhang Lihong Yin Yuepu Pu Zaozao Chen Geyu Liang Dynamic non-coding RNA biomarker reveals lung injury and repair induced by polystyrene nanoplastics Environment International Polystyrene nanoplastics Lung injury Time-resolved analysis Transitional cells non-coding RNA |
| title | Dynamic non-coding RNA biomarker reveals lung injury and repair induced by polystyrene nanoplastics |
| title_full | Dynamic non-coding RNA biomarker reveals lung injury and repair induced by polystyrene nanoplastics |
| title_fullStr | Dynamic non-coding RNA biomarker reveals lung injury and repair induced by polystyrene nanoplastics |
| title_full_unstemmed | Dynamic non-coding RNA biomarker reveals lung injury and repair induced by polystyrene nanoplastics |
| title_short | Dynamic non-coding RNA biomarker reveals lung injury and repair induced by polystyrene nanoplastics |
| title_sort | dynamic non coding rna biomarker reveals lung injury and repair induced by polystyrene nanoplastics |
| topic | Polystyrene nanoplastics Lung injury Time-resolved analysis Transitional cells non-coding RNA |
| url | http://www.sciencedirect.com/science/article/pii/S0160412025000170 |
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