Human Urine-Derived Stem Cells: Potential for Cell-Based Therapy of Cartilage Defects
Stem cell therapy is considered an optimistic approach to replace current treatments for cartilage defects. Recently, human urine-derived stem cells (hUSCs), which are isolated from the urine, are studied as a promising candidate for many tissue engineering therapies due to their multipotency and su...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2018/4686259 |
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| author | Long Chen Lang Li Fei Xing Jing Peng Kun Peng Yuanzheng Wang Zhou Xiang |
| author_facet | Long Chen Lang Li Fei Xing Jing Peng Kun Peng Yuanzheng Wang Zhou Xiang |
| author_sort | Long Chen |
| collection | DOAJ |
| description | Stem cell therapy is considered an optimistic approach to replace current treatments for cartilage defects. Recently, human urine-derived stem cells (hUSCs), which are isolated from the urine, are studied as a promising candidate for many tissue engineering therapies due to their multipotency and sufficient proliferation activities. However, it has not yet been reported whether hUSCs can be employed in cartilage defects. In this study, we revealed that induced hUSCs expressed chondrogenic-related proteins, including aggrecan and collagen II, and their gene expression levels were upregulated in vitro. Moreover, we combined hUSCs with hyaluronic acid (HA) and injected hUSCs-HA into a rabbit knee joint with cartilage defect. Twelve weeks after the injection, the histologic analyses (HE, toluidine blue, and Masson trichrome staining), immunohistochemistry (aggrecan and collagen II), and histologic grade of the sample indicated that hUSCs-HA could stimulate much more neocartilage formation compared with hUSCs alone, pure HA, and saline, which only induced the modest cartilage regeneration. In this study, we demonstrated that hUSCs could be a potential cell source for stem cell therapies to treat cartilage-related defects in the future. |
| format | Article |
| id | doaj-art-899cdbfffa8641bb9ccaa96f80b7999b |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-899cdbfffa8641bb9ccaa96f80b7999b2025-02-03T01:07:45ZengWileyStem Cells International1687-966X1687-96782018-01-01201810.1155/2018/46862594686259Human Urine-Derived Stem Cells: Potential for Cell-Based Therapy of Cartilage DefectsLong Chen0Lang Li1Fei Xing2Jing Peng3Kun Peng4Yuanzheng Wang5Zhou Xiang6Department of Orthopedics, West China Hospital, Sichuan University, Chengdu, Sichuan, ChinaDepartment of Orthopedics, West China Hospital, Sichuan University, Chengdu, Sichuan, ChinaDepartment of Orthopedics, West China Hospital, Sichuan University, Chengdu, Sichuan, ChinaDepartment of Orthopedics, West China Hospital, Sichuan University, Chengdu, Sichuan, ChinaDepartment of Orthopedics, The Second Affiliated Hospital of Nanchang University, Nanchang, ChinaDepartment of Orthopedics, Guizhou Provincial People’s Hospital, Guiyang, Guizhou, ChinaDepartment of Orthopedics, West China Hospital, Sichuan University, Chengdu, Sichuan, ChinaStem cell therapy is considered an optimistic approach to replace current treatments for cartilage defects. Recently, human urine-derived stem cells (hUSCs), which are isolated from the urine, are studied as a promising candidate for many tissue engineering therapies due to their multipotency and sufficient proliferation activities. However, it has not yet been reported whether hUSCs can be employed in cartilage defects. In this study, we revealed that induced hUSCs expressed chondrogenic-related proteins, including aggrecan and collagen II, and their gene expression levels were upregulated in vitro. Moreover, we combined hUSCs with hyaluronic acid (HA) and injected hUSCs-HA into a rabbit knee joint with cartilage defect. Twelve weeks after the injection, the histologic analyses (HE, toluidine blue, and Masson trichrome staining), immunohistochemistry (aggrecan and collagen II), and histologic grade of the sample indicated that hUSCs-HA could stimulate much more neocartilage formation compared with hUSCs alone, pure HA, and saline, which only induced the modest cartilage regeneration. In this study, we demonstrated that hUSCs could be a potential cell source for stem cell therapies to treat cartilage-related defects in the future.http://dx.doi.org/10.1155/2018/4686259 |
| spellingShingle | Long Chen Lang Li Fei Xing Jing Peng Kun Peng Yuanzheng Wang Zhou Xiang Human Urine-Derived Stem Cells: Potential for Cell-Based Therapy of Cartilage Defects Stem Cells International |
| title | Human Urine-Derived Stem Cells: Potential for Cell-Based Therapy of Cartilage Defects |
| title_full | Human Urine-Derived Stem Cells: Potential for Cell-Based Therapy of Cartilage Defects |
| title_fullStr | Human Urine-Derived Stem Cells: Potential for Cell-Based Therapy of Cartilage Defects |
| title_full_unstemmed | Human Urine-Derived Stem Cells: Potential for Cell-Based Therapy of Cartilage Defects |
| title_short | Human Urine-Derived Stem Cells: Potential for Cell-Based Therapy of Cartilage Defects |
| title_sort | human urine derived stem cells potential for cell based therapy of cartilage defects |
| url | http://dx.doi.org/10.1155/2018/4686259 |
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