Expression of LGI1 Impairs Proliferation and Survival of HeLa Cells
The LGI1 gene was suggested to function as tumor suppressor for its ability to reduce malignant features of glioblastoma cells. In support to this proposal were the findings that overexpression of LGI1 in neuroblastoma cells inhibited proliferation and induced apoptosis. In this study we performed s...
Saved in:
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2009-01-01
|
| Series: | International Journal of Cell Biology |
| Online Access: | http://dx.doi.org/10.1155/2009/417197 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832556619049730048 |
|---|---|
| author | Nadia Gabellini Valentina Masola |
| author_facet | Nadia Gabellini Valentina Masola |
| author_sort | Nadia Gabellini |
| collection | DOAJ |
| description | The LGI1 gene was suggested to function as tumor suppressor for its ability to reduce malignant features of glioblastoma cells. In support to this proposal were the findings that overexpression of LGI1 in neuroblastoma cells inhibited proliferation and induced apoptosis. In this study we performed stable LGI1 expression in HeLa cells to examine whether the noxious effect of LGI1 might be extended to cancer cells of diverse origin. HeLa cell clones stably expressing LGI1 exhibited a significant impairment of proliferation and a consistent increase of cell death when compared with control cells lacking expression of LGI1. Expression of LGI1 increased the activity of apoptosis effectors caspase-3/7; furthermore it downregulated the antiapoptotic BCL2 gene and upregulated the proapoptotic BAX gene expression, suggesting that the cause of HeLa cells death might be an increased susceptibility to apoptosis induced by LGI1. The results suggested that LGI1 is capable to restrain growth and survival of adenocarcinoma cells such as HeLa. |
| format | Article |
| id | doaj-art-8998b1d276d8406d8a9f19e3b2bbdf13 |
| institution | Kabale University |
| issn | 1687-8876 1687-8884 |
| language | English |
| publishDate | 2009-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Cell Biology |
| spelling | doaj-art-8998b1d276d8406d8a9f19e3b2bbdf132025-02-03T05:44:55ZengWileyInternational Journal of Cell Biology1687-88761687-88842009-01-01200910.1155/2009/417197417197Expression of LGI1 Impairs Proliferation and Survival of HeLa CellsNadia Gabellini0Valentina Masola1Department of Biological Chemistry, University of Padova, 35121 Padova, ItalyDepartment of Biological Chemistry, University of Padova, 35121 Padova, ItalyThe LGI1 gene was suggested to function as tumor suppressor for its ability to reduce malignant features of glioblastoma cells. In support to this proposal were the findings that overexpression of LGI1 in neuroblastoma cells inhibited proliferation and induced apoptosis. In this study we performed stable LGI1 expression in HeLa cells to examine whether the noxious effect of LGI1 might be extended to cancer cells of diverse origin. HeLa cell clones stably expressing LGI1 exhibited a significant impairment of proliferation and a consistent increase of cell death when compared with control cells lacking expression of LGI1. Expression of LGI1 increased the activity of apoptosis effectors caspase-3/7; furthermore it downregulated the antiapoptotic BCL2 gene and upregulated the proapoptotic BAX gene expression, suggesting that the cause of HeLa cells death might be an increased susceptibility to apoptosis induced by LGI1. The results suggested that LGI1 is capable to restrain growth and survival of adenocarcinoma cells such as HeLa.http://dx.doi.org/10.1155/2009/417197 |
| spellingShingle | Nadia Gabellini Valentina Masola Expression of LGI1 Impairs Proliferation and Survival of HeLa Cells International Journal of Cell Biology |
| title | Expression of LGI1 Impairs Proliferation and Survival of HeLa Cells |
| title_full | Expression of LGI1 Impairs Proliferation and Survival of HeLa Cells |
| title_fullStr | Expression of LGI1 Impairs Proliferation and Survival of HeLa Cells |
| title_full_unstemmed | Expression of LGI1 Impairs Proliferation and Survival of HeLa Cells |
| title_short | Expression of LGI1 Impairs Proliferation and Survival of HeLa Cells |
| title_sort | expression of lgi1 impairs proliferation and survival of hela cells |
| url | http://dx.doi.org/10.1155/2009/417197 |
| work_keys_str_mv | AT nadiagabellini expressionoflgi1impairsproliferationandsurvivalofhelacells AT valentinamasola expressionoflgi1impairsproliferationandsurvivalofhelacells |