Characterization of pPCP1 Plasmids in Yersinia pestis Strains Isolated from the Former Soviet Union
Complete sequences of 9.5-kb pPCP1 plasmids in three Yersinia pestis strains from the former Soviet Union (FSU) were determined and compared with those of pPCP1 plasmids in three well-characterized, non-FSU Y. pestis strains (KIM, CO92, and 91001). Two of the FSU plasmids were from strains C2614 and...
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| Format: | Article |
| Language: | English |
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Wiley
2010-01-01
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| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2010/760819 |
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| author | Chythanya Rajanna Tamara Revazishvili Mohammed H. Rashid Svetlana Chubinidze Lela Bakanidze Shota Tsanava Paata Imnadze Kimberly A. Bishop-Lilly Shanmuga Sozhamannan Henry S. Gibbons J. Glenn Morris Alexander Sulakvelidze |
| author_facet | Chythanya Rajanna Tamara Revazishvili Mohammed H. Rashid Svetlana Chubinidze Lela Bakanidze Shota Tsanava Paata Imnadze Kimberly A. Bishop-Lilly Shanmuga Sozhamannan Henry S. Gibbons J. Glenn Morris Alexander Sulakvelidze |
| author_sort | Chythanya Rajanna |
| collection | DOAJ |
| description | Complete sequences of 9.5-kb pPCP1 plasmids in three Yersinia pestis strains from the former Soviet Union (FSU) were determined and compared with those of pPCP1 plasmids in three well-characterized, non-FSU Y. pestis strains (KIM, CO92, and 91001). Two of the FSU plasmids were from strains C2614 and C2944, isolated from plague foci in Russia, and one plasmid was from strain C790 from Kyrgyzstan. Sequence analyses identified four sequence types among the six plasmids. The pPCP1 plasmids in the FSU strains were most genetically related to the pPCP1 plasmid in the KIM strain and least related to the pPCP1 plasmid in Y. pestis 91001. The FSU strains generally had larger pPCP1 plasmid copy numbers compared to strain CO92. Expression of the plasmid's pla gene was significantly (P≤.05) higher in strain C2944 than in strain CO92. Given pla's role in Y. pestis virulence, this difference may have important implications for the strain's virulence. |
| format | Article |
| id | doaj-art-89701cca7ae54e419fb16b3761702e8a |
| institution | Kabale University |
| issn | 1687-918X 1687-9198 |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-89701cca7ae54e419fb16b3761702e8a2025-02-03T01:00:30ZengWileyInternational Journal of Microbiology1687-918X1687-91982010-01-01201010.1155/2010/760819760819Characterization of pPCP1 Plasmids in Yersinia pestis Strains Isolated from the Former Soviet UnionChythanya Rajanna0Tamara Revazishvili1Mohammed H. Rashid2Svetlana Chubinidze3Lela Bakanidze4Shota Tsanava5Paata Imnadze6Kimberly A. Bishop-Lilly7Shanmuga Sozhamannan8Henry S. Gibbons9J. Glenn Morris10Alexander Sulakvelidze11Emerging Pathogens Institute and Department of Molecular Genetics and Microbiology, University of Florida College of Medicine, Gainesville, FL 32610, USAEmerging Pathogens Institute and Department of Molecular Genetics and Microbiology, University of Florida College of Medicine, Gainesville, FL 32610, USAEmerging Pathogens Institute and Department of Molecular Genetics and Microbiology, University of Florida College of Medicine, Gainesville, FL 32610, USANational Center for Disease Control and Public Health, Georgian Ministry of Health, 0177 Tbilisi, GeorgiaNational Center for Disease Control and Public Health, Georgian Ministry of Health, 0177 Tbilisi, GeorgiaNational Center for Disease Control and Public Health, Georgian Ministry of Health, 0177 Tbilisi, GeorgiaNational Center for Disease Control and Public Health, Georgian Ministry of Health, 0177 Tbilisi, GeorgiaNaval Medical Center Annex, Rockville, MD 20852, USANaval Medical Center Annex, Rockville, MD 20852, USAUS Army Edgewood Chemical Biological Center, Aberdeen Proving Ground, MD 21010, USAEmerging Pathogens Institute and Department of Molecular Genetics and Microbiology, University of Florida College of Medicine, Gainesville, FL 32610, USAEmerging Pathogens Institute and Department of Molecular Genetics and Microbiology, University of Florida College of Medicine, Gainesville, FL 32610, USAComplete sequences of 9.5-kb pPCP1 plasmids in three Yersinia pestis strains from the former Soviet Union (FSU) were determined and compared with those of pPCP1 plasmids in three well-characterized, non-FSU Y. pestis strains (KIM, CO92, and 91001). Two of the FSU plasmids were from strains C2614 and C2944, isolated from plague foci in Russia, and one plasmid was from strain C790 from Kyrgyzstan. Sequence analyses identified four sequence types among the six plasmids. The pPCP1 plasmids in the FSU strains were most genetically related to the pPCP1 plasmid in the KIM strain and least related to the pPCP1 plasmid in Y. pestis 91001. The FSU strains generally had larger pPCP1 plasmid copy numbers compared to strain CO92. Expression of the plasmid's pla gene was significantly (P≤.05) higher in strain C2944 than in strain CO92. Given pla's role in Y. pestis virulence, this difference may have important implications for the strain's virulence.http://dx.doi.org/10.1155/2010/760819 |
| spellingShingle | Chythanya Rajanna Tamara Revazishvili Mohammed H. Rashid Svetlana Chubinidze Lela Bakanidze Shota Tsanava Paata Imnadze Kimberly A. Bishop-Lilly Shanmuga Sozhamannan Henry S. Gibbons J. Glenn Morris Alexander Sulakvelidze Characterization of pPCP1 Plasmids in Yersinia pestis Strains Isolated from the Former Soviet Union International Journal of Microbiology |
| title | Characterization of pPCP1 Plasmids in Yersinia pestis Strains Isolated from the Former Soviet Union |
| title_full | Characterization of pPCP1 Plasmids in Yersinia pestis Strains Isolated from the Former Soviet Union |
| title_fullStr | Characterization of pPCP1 Plasmids in Yersinia pestis Strains Isolated from the Former Soviet Union |
| title_full_unstemmed | Characterization of pPCP1 Plasmids in Yersinia pestis Strains Isolated from the Former Soviet Union |
| title_short | Characterization of pPCP1 Plasmids in Yersinia pestis Strains Isolated from the Former Soviet Union |
| title_sort | characterization of ppcp1 plasmids in yersinia pestis strains isolated from the former soviet union |
| url | http://dx.doi.org/10.1155/2010/760819 |
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