Antioxidant and hepatoprotective effects of Hypsizygus ulmarius polysaccharide on alcoholic liver injury in rats
Hypsizygus ulmarius polysaccharide (HUP) is a water-soluble polysaccharide obtained by hot water extraction, followed by precipitation and deproteinization. The characteristics of HUP, antioxidant activity and liver protection against alcohol-induced liver damage were studied. Structural characteris...
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Tsinghua University Press
2021-07-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453021000628 |
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| author | Sudha Govindan Angu Jayabal Jayasakthi Shanmugam Prasanna Ramani |
| author_facet | Sudha Govindan Angu Jayabal Jayasakthi Shanmugam Prasanna Ramani |
| author_sort | Sudha Govindan |
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| description | Hypsizygus ulmarius polysaccharide (HUP) is a water-soluble polysaccharide obtained by hot water extraction, followed by precipitation and deproteinization. The characteristics of HUP, antioxidant activity and liver protection against alcohol-induced liver damage were studied. Structural characteristics indicate that the HUP is a pyran-type polysaccharide with a molecular weight of 2076 Da. In antioxidant scavenging assay, HUP showed moderate DMPD radical scavenging activity, cupric ion reducing antioxidant capacity and inhibitory effect against lipid peroxidation in a dose-dependent manner. Regarding in vivo hepatoprotective activity, compared with the ethanol induction group, pre-treatment of low and high doses of HUP significantly reduced the behaviours of serum enzymes, lowered the levels of hepatic oxidative stress markers, restored the levels of biochemical constituents, enhanced the levels of liver and serum enzymatic antioxidants and non-enzymatic antioxidants, and improved the serum lipid levels of alcohol-intoxicated rats. The hepatoprotective effect of HUP was comparable to positive control silymarin. Besides, HUP pre-treatment significantly normalized the histopathological changes induced by ethanol. The results indicate that HUP could be used as a functional food and may protect the biological system from oxidative stress through its antioxidant activity, thus having a significant protective effect on acute alcoholic liver injury. |
| format | Article |
| id | doaj-art-8969510e89f447ecb728a3ae9f0214a7 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2021-07-01 |
| publisher | Tsinghua University Press |
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| series | Food Science and Human Wellness |
| spelling | doaj-art-8969510e89f447ecb728a3ae9f0214a72025-02-03T02:40:51ZengTsinghua University PressFood Science and Human Wellness2213-45302021-07-01104523535Antioxidant and hepatoprotective effects of Hypsizygus ulmarius polysaccharide on alcoholic liver injury in ratsSudha Govindan0Angu Jayabal1Jayasakthi Shanmugam2Prasanna Ramani3Department of Biochemistry, School of Biosciences, Periyar University, Salem, India; Corresponding author.Department of Biochemistry, Kongunadu Arts and Science College, Bharathiar University, Coimbatore, IndiaDepartment of Biochemistry, Kongunadu Arts and Science College, Bharathiar University, Coimbatore, IndiaDhanvanthri Lab, Department of Sciences, Amrita School of Engineering, Coimbatore, Amrita Vishwa Vidyapeetham, India; Center of Excellence in Advanced Materials & Green Technologies (CoE–AMGT), Amrita School of Engineering, Coimbatore, Amrita Vishwa Vidyapeetham, IndiaHypsizygus ulmarius polysaccharide (HUP) is a water-soluble polysaccharide obtained by hot water extraction, followed by precipitation and deproteinization. The characteristics of HUP, antioxidant activity and liver protection against alcohol-induced liver damage were studied. Structural characteristics indicate that the HUP is a pyran-type polysaccharide with a molecular weight of 2076 Da. In antioxidant scavenging assay, HUP showed moderate DMPD radical scavenging activity, cupric ion reducing antioxidant capacity and inhibitory effect against lipid peroxidation in a dose-dependent manner. Regarding in vivo hepatoprotective activity, compared with the ethanol induction group, pre-treatment of low and high doses of HUP significantly reduced the behaviours of serum enzymes, lowered the levels of hepatic oxidative stress markers, restored the levels of biochemical constituents, enhanced the levels of liver and serum enzymatic antioxidants and non-enzymatic antioxidants, and improved the serum lipid levels of alcohol-intoxicated rats. The hepatoprotective effect of HUP was comparable to positive control silymarin. Besides, HUP pre-treatment significantly normalized the histopathological changes induced by ethanol. The results indicate that HUP could be used as a functional food and may protect the biological system from oxidative stress through its antioxidant activity, thus having a significant protective effect on acute alcoholic liver injury.http://www.sciencedirect.com/science/article/pii/S2213453021000628Hypsizygus ulmarius polysaccharideAntioxidant activityHepatoprotective behaviourEthanol consumptionLiver damage |
| spellingShingle | Sudha Govindan Angu Jayabal Jayasakthi Shanmugam Prasanna Ramani Antioxidant and hepatoprotective effects of Hypsizygus ulmarius polysaccharide on alcoholic liver injury in rats Food Science and Human Wellness Hypsizygus ulmarius polysaccharide Antioxidant activity Hepatoprotective behaviour Ethanol consumption Liver damage |
| title | Antioxidant and hepatoprotective effects of Hypsizygus ulmarius polysaccharide on alcoholic liver injury in rats |
| title_full | Antioxidant and hepatoprotective effects of Hypsizygus ulmarius polysaccharide on alcoholic liver injury in rats |
| title_fullStr | Antioxidant and hepatoprotective effects of Hypsizygus ulmarius polysaccharide on alcoholic liver injury in rats |
| title_full_unstemmed | Antioxidant and hepatoprotective effects of Hypsizygus ulmarius polysaccharide on alcoholic liver injury in rats |
| title_short | Antioxidant and hepatoprotective effects of Hypsizygus ulmarius polysaccharide on alcoholic liver injury in rats |
| title_sort | antioxidant and hepatoprotective effects of hypsizygus ulmarius polysaccharide on alcoholic liver injury in rats |
| topic | Hypsizygus ulmarius polysaccharide Antioxidant activity Hepatoprotective behaviour Ethanol consumption Liver damage |
| url | http://www.sciencedirect.com/science/article/pii/S2213453021000628 |
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