Molecular Monitoring of Flavivirus Infections (West Nile Virus and Dengue Virus) Among Culicidae Mosquitoes in Southern Iran
Background: Mosquitoes (Diptera: Culicidae) are significant vectors of mosquito-borne diseases prevalent across temperate and tropical regions. Understanding the abundance and distribution of these vectors is essential for studying the ecoepidemiology of mosquito-borne diseases.Methods: This cross-s...
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Shiraz University of Medical Sciences
2025-01-01
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| Series: | Journal of Health Sciences and Surveillance System |
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| Online Access: | https://jhsss.sums.ac.ir/article_50665_d93d22b4d5fdb4b6c109042aa4ecb6d3.pdf |
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| author | Masoumeh Amin Hamideh Edalat Hamidreza Basseri Morteza Zaim Kourosh Azizi Mostafa Salehi- Vaziri Sahar Khakifirouz Mohsen Kalantari Mozaffar Vahedi Saideh Yousefi Parisa Amin |
| author_facet | Masoumeh Amin Hamideh Edalat Hamidreza Basseri Morteza Zaim Kourosh Azizi Mostafa Salehi- Vaziri Sahar Khakifirouz Mohsen Kalantari Mozaffar Vahedi Saideh Yousefi Parisa Amin |
| author_sort | Masoumeh Amin |
| collection | DOAJ |
| description | Background: Mosquitoes (Diptera: Culicidae) are significant vectors of mosquito-borne diseases prevalent across temperate and tropical regions. Understanding the abundance and distribution of these vectors is essential for studying the ecoepidemiology of mosquito-borne diseases.Methods: This cross-sectional study aimed to identify mosquito populations (Culicidae) and conduct molecular analysis for Flavivirus infections, explicitly focusing on West Nile Virus (WNV) and Dengue Virus (DENV) in Fars Province, Southern Iran. Mosquitoes were collected from 23 stations across urban and rural areas and migratory bird habitats in five counties, using hand catch with manual aspirators, CDC light traps with CO2, and human and animal bait methods from April to December 2018. Molecular screening for Flavivirus RNA, including WNV and DENV, was performed using a specific PCR technique.Results: 8212 adult mosquitoes were collected, representing four genera and nine species. The most prevalent species were Culex pipiens (54.20%), Culiseta longiareolata (30.40%), and Culex sinaiticus (10.25%). Molecular screening was conducted on the 8212 mosquitoes grouped into 150 pools based on sex, species, and trapping location. None of the pools tested were positive for Flavivirus RNA.Conclusion: This study highlights the importance of monitoring mosquito species distribution and emphasizes the need for enhanced Flavivirus surveillance and long-term monitoring programs in the region to understand the risk of disease transmission better. |
| format | Article |
| id | doaj-art-896676f53cb14eda8c39bc905ae4feb5 |
| institution | Kabale University |
| issn | 2345-2218 2345-3893 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Shiraz University of Medical Sciences |
| record_format | Article |
| series | Journal of Health Sciences and Surveillance System |
| spelling | doaj-art-896676f53cb14eda8c39bc905ae4feb52025-02-02T06:03:20ZengShiraz University of Medical SciencesJournal of Health Sciences and Surveillance System2345-22182345-38932025-01-01131687410.30476/jhsss.2024.100128.182650665Molecular Monitoring of Flavivirus Infections (West Nile Virus and Dengue Virus) Among Culicidae Mosquitoes in Southern IranMasoumeh Amin0Hamideh Edalat1Hamidreza Basseri2Morteza Zaim3Kourosh Azizi4Mostafa Salehi- Vaziri5Sahar Khakifirouz6Mohsen Kalantari7Mozaffar Vahedi8Saideh Yousefi9Parisa Amin10Department of Vector Biology and Control of Diseases, School of Health, Shiraz University of Medical Sciences, Shiraz, IranDepartment of Vector Biology and Control of Diseases, School of Health, Tehran University of Medical Sciences, Tehran, IranDepartment of Vector Biology and Control of Diseases, School of Health, Tehran University of Medical Sciences, Tehran, IranDepartment of Vector Biology and Control of Diseases, School of Health, Tehran University of Medical Sciences, Tehran, IranResearch Center for Health Sciences, Institute of Health, Department of Vector Biology and Control of Diseases, School of Health, Shiraz University of Medical Sciences, Shiraz, IranDepartment of Arboviruses and Viral Hemorrhagic Fevers, Pasteur Institute of IranDepartment of Arboviruses and Viral Hemorrhagic Fevers, Pasteur Institute of IranResearch Center for Health Sciences, Institute of Health, Department of Vector Biology and Control of Diseases, School of Health, Shiraz University of Medical Sciences, Shiraz, IranStudent Research Committee, Department of Vector Biology and Control of Diseases, School of Health, Shiraz University of Medical Sciences, Shiraz, IranSirjan School of Medical Sciences, Sirjan, IranDepartment of Physiotherapy and Rehabilitation, Yeditepe University, Istanbul, TurkeyBackground: Mosquitoes (Diptera: Culicidae) are significant vectors of mosquito-borne diseases prevalent across temperate and tropical regions. Understanding the abundance and distribution of these vectors is essential for studying the ecoepidemiology of mosquito-borne diseases.Methods: This cross-sectional study aimed to identify mosquito populations (Culicidae) and conduct molecular analysis for Flavivirus infections, explicitly focusing on West Nile Virus (WNV) and Dengue Virus (DENV) in Fars Province, Southern Iran. Mosquitoes were collected from 23 stations across urban and rural areas and migratory bird habitats in five counties, using hand catch with manual aspirators, CDC light traps with CO2, and human and animal bait methods from April to December 2018. Molecular screening for Flavivirus RNA, including WNV and DENV, was performed using a specific PCR technique.Results: 8212 adult mosquitoes were collected, representing four genera and nine species. The most prevalent species were Culex pipiens (54.20%), Culiseta longiareolata (30.40%), and Culex sinaiticus (10.25%). Molecular screening was conducted on the 8212 mosquitoes grouped into 150 pools based on sex, species, and trapping location. None of the pools tested were positive for Flavivirus RNA.Conclusion: This study highlights the importance of monitoring mosquito species distribution and emphasizes the need for enhanced Flavivirus surveillance and long-term monitoring programs in the region to understand the risk of disease transmission better.https://jhsss.sums.ac.ir/article_50665_d93d22b4d5fdb4b6c109042aa4ecb6d3.pdffarsflavivirusiranmosquito |
| spellingShingle | Masoumeh Amin Hamideh Edalat Hamidreza Basseri Morteza Zaim Kourosh Azizi Mostafa Salehi- Vaziri Sahar Khakifirouz Mohsen Kalantari Mozaffar Vahedi Saideh Yousefi Parisa Amin Molecular Monitoring of Flavivirus Infections (West Nile Virus and Dengue Virus) Among Culicidae Mosquitoes in Southern Iran Journal of Health Sciences and Surveillance System fars flavivirus iran mosquito |
| title | Molecular Monitoring of Flavivirus Infections (West Nile Virus and Dengue Virus) Among Culicidae Mosquitoes in Southern Iran |
| title_full | Molecular Monitoring of Flavivirus Infections (West Nile Virus and Dengue Virus) Among Culicidae Mosquitoes in Southern Iran |
| title_fullStr | Molecular Monitoring of Flavivirus Infections (West Nile Virus and Dengue Virus) Among Culicidae Mosquitoes in Southern Iran |
| title_full_unstemmed | Molecular Monitoring of Flavivirus Infections (West Nile Virus and Dengue Virus) Among Culicidae Mosquitoes in Southern Iran |
| title_short | Molecular Monitoring of Flavivirus Infections (West Nile Virus and Dengue Virus) Among Culicidae Mosquitoes in Southern Iran |
| title_sort | molecular monitoring of flavivirus infections west nile virus and dengue virus among culicidae mosquitoes in southern iran |
| topic | fars flavivirus iran mosquito |
| url | https://jhsss.sums.ac.ir/article_50665_d93d22b4d5fdb4b6c109042aa4ecb6d3.pdf |
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