Evaluation of the Tyrosine and Dopamine Serum Levels in Experimental Infected BALB/c Mice with Chronic Toxoplasmosis
Background. Toxoplasma parasite alters the transduction of neurotransmitter signals and leads to changes in the level of brain neurotransmitters including tyrosine and dopamine, so behavior changes can occur in infected hosts. Based on this concept, this study was conducted for evaluation of the tyr...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2021-01-01
|
| Series: | Journal of Parasitology Research |
| Online Access: | http://dx.doi.org/10.1155/2021/5511516 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832560678964035584 |
|---|---|
| author | Mehdi Mirzaeipour Fattaneh Mikaeili Qasem Asgari Mohammad Nohtani Sajad Rashidi Mohammad Saleh Bahreini |
| author_facet | Mehdi Mirzaeipour Fattaneh Mikaeili Qasem Asgari Mohammad Nohtani Sajad Rashidi Mohammad Saleh Bahreini |
| author_sort | Mehdi Mirzaeipour |
| collection | DOAJ |
| description | Background. Toxoplasma parasite alters the transduction of neurotransmitter signals and leads to changes in the level of brain neurotransmitters including tyrosine and dopamine, so behavior changes can occur in infected hosts. Based on this concept, this study was conducted for evaluation of the tyrosine and dopamine serum levels in infected mice with chronic toxoplasmosis. Materials and Methods. Toxoplasma gondii (Prugniaud strain II) was injected intraperitoneally into BALB/c mice to induce chronic toxoplasmosis. Modified agglutination test (MAT), polymerase chain reaction (PCR), and microscopic methods were conducted to confirm the induction of chronic toxoplasmosis. The infected mouse sera were separated at days 40, 50, 60, 70, and 80 for evaluation of tyrosine and dopamine serum levels using high-performance liquid chromatography (HPLC). Results. Microscopic methods confirmed the formation of the Toxoplasma cysts in mouse tissues. Inducing chronic toxoplasmosis is also confirmed by MAT, PCR, and histological methods. HPLC results indicated a decrease in serum tyrosine level at day 40 in infected mice in comparison to control, and the levels were too low to be measured at other times. However, a significantly high serum dopamine level was observed that gradually increased after parasite inoculation. Conclusions. No detection of tyrosine level in most of the sample groups is probably related to the very low concentration of tyrosine in sera. However, low concentration of tyrosine at day 40 and increase of dopamine in most of the sample groups suggest the production of dopamine from tyrosine due to the presence of Toxoplasma in infected mice. |
| format | Article |
| id | doaj-art-895dbfb2571f452ca6511da40940d8e3 |
| institution | Kabale University |
| issn | 2090-0023 2090-0031 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Parasitology Research |
| spelling | doaj-art-895dbfb2571f452ca6511da40940d8e32025-02-03T01:26:59ZengWileyJournal of Parasitology Research2090-00232090-00312021-01-01202110.1155/2021/55115165511516Evaluation of the Tyrosine and Dopamine Serum Levels in Experimental Infected BALB/c Mice with Chronic ToxoplasmosisMehdi Mirzaeipour0Fattaneh Mikaeili1Qasem Asgari2Mohammad Nohtani3Sajad Rashidi4Mohammad Saleh Bahreini5Department of Parasitology and Mycology, Medicine Faculty, Shiraz University of Medical Sciences, Shiraz, IranDepartment of Parasitology and Mycology, Medicine Faculty, Shiraz University of Medical Sciences, Shiraz, IranDepartment of Parasitology and Mycology, Medicine Faculty, Shiraz University of Medical Sciences, Shiraz, IranDepartment of Parasitology and Mycology, Medicine Faculty, Shiraz University of Medical Sciences, Shiraz, IranDepartment of Parasitology and Mycology, Medicine Faculty, Shiraz University of Medical Sciences, Shiraz, IranDepartment of Parasitology and Mycology, Medicine Faculty, Shiraz University of Medical Sciences, Shiraz, IranBackground. Toxoplasma parasite alters the transduction of neurotransmitter signals and leads to changes in the level of brain neurotransmitters including tyrosine and dopamine, so behavior changes can occur in infected hosts. Based on this concept, this study was conducted for evaluation of the tyrosine and dopamine serum levels in infected mice with chronic toxoplasmosis. Materials and Methods. Toxoplasma gondii (Prugniaud strain II) was injected intraperitoneally into BALB/c mice to induce chronic toxoplasmosis. Modified agglutination test (MAT), polymerase chain reaction (PCR), and microscopic methods were conducted to confirm the induction of chronic toxoplasmosis. The infected mouse sera were separated at days 40, 50, 60, 70, and 80 for evaluation of tyrosine and dopamine serum levels using high-performance liquid chromatography (HPLC). Results. Microscopic methods confirmed the formation of the Toxoplasma cysts in mouse tissues. Inducing chronic toxoplasmosis is also confirmed by MAT, PCR, and histological methods. HPLC results indicated a decrease in serum tyrosine level at day 40 in infected mice in comparison to control, and the levels were too low to be measured at other times. However, a significantly high serum dopamine level was observed that gradually increased after parasite inoculation. Conclusions. No detection of tyrosine level in most of the sample groups is probably related to the very low concentration of tyrosine in sera. However, low concentration of tyrosine at day 40 and increase of dopamine in most of the sample groups suggest the production of dopamine from tyrosine due to the presence of Toxoplasma in infected mice.http://dx.doi.org/10.1155/2021/5511516 |
| spellingShingle | Mehdi Mirzaeipour Fattaneh Mikaeili Qasem Asgari Mohammad Nohtani Sajad Rashidi Mohammad Saleh Bahreini Evaluation of the Tyrosine and Dopamine Serum Levels in Experimental Infected BALB/c Mice with Chronic Toxoplasmosis Journal of Parasitology Research |
| title | Evaluation of the Tyrosine and Dopamine Serum Levels in Experimental Infected BALB/c Mice with Chronic Toxoplasmosis |
| title_full | Evaluation of the Tyrosine and Dopamine Serum Levels in Experimental Infected BALB/c Mice with Chronic Toxoplasmosis |
| title_fullStr | Evaluation of the Tyrosine and Dopamine Serum Levels in Experimental Infected BALB/c Mice with Chronic Toxoplasmosis |
| title_full_unstemmed | Evaluation of the Tyrosine and Dopamine Serum Levels in Experimental Infected BALB/c Mice with Chronic Toxoplasmosis |
| title_short | Evaluation of the Tyrosine and Dopamine Serum Levels in Experimental Infected BALB/c Mice with Chronic Toxoplasmosis |
| title_sort | evaluation of the tyrosine and dopamine serum levels in experimental infected balb c mice with chronic toxoplasmosis |
| url | http://dx.doi.org/10.1155/2021/5511516 |
| work_keys_str_mv | AT mehdimirzaeipour evaluationofthetyrosineanddopamineserumlevelsinexperimentalinfectedbalbcmicewithchronictoxoplasmosis AT fattanehmikaeili evaluationofthetyrosineanddopamineserumlevelsinexperimentalinfectedbalbcmicewithchronictoxoplasmosis AT qasemasgari evaluationofthetyrosineanddopamineserumlevelsinexperimentalinfectedbalbcmicewithchronictoxoplasmosis AT mohammadnohtani evaluationofthetyrosineanddopamineserumlevelsinexperimentalinfectedbalbcmicewithchronictoxoplasmosis AT sajadrashidi evaluationofthetyrosineanddopamineserumlevelsinexperimentalinfectedbalbcmicewithchronictoxoplasmosis AT mohammadsalehbahreini evaluationofthetyrosineanddopamineserumlevelsinexperimentalinfectedbalbcmicewithchronictoxoplasmosis |