Effect of Oxygen and Redox Potential on Glucose Fermentation in Thermotoga maritima under Controlled Physicochemical Conditions
Batch cultures of Thermotoga maritima were performed in a bioreactor equipped with instruments adapted for experiments performed at 80∘C to mimic the fluctuating oxidative conditions in the hot ecosystems it inhabits. When grown anaerobically on glucose, T. maritima was shown to significantly decrea...
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| Format: | Article |
| Language: | English |
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Wiley
2010-01-01
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| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2010/896510 |
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| author | Raja Lakhal Richard Auria Sylvain Davidson Bernard Ollivier Alain Dolla Moktar Hamdi Yannick Combet-Blanc |
| author_facet | Raja Lakhal Richard Auria Sylvain Davidson Bernard Ollivier Alain Dolla Moktar Hamdi Yannick Combet-Blanc |
| author_sort | Raja Lakhal |
| collection | DOAJ |
| description | Batch cultures of Thermotoga maritima were performed in a bioreactor equipped with instruments adapted for experiments performed at 80∘C to mimic the fluctuating oxidative conditions in the hot ecosystems it inhabits. When grown anaerobically on glucose, T. maritima was shown to significantly decrease the redox potential (Eh) of the culture medium down to about −480 mV, as long as glucose was available. Addition of oxygen into T. maritima cultures during the stationary growth phase led to a drastic reduction in glucose consumption rate. However, although oxygen was toxic, our experiment unambiguously proved that T. maritima was able to consume it during a 12-hour exposure period. Furthermore, a shift in glucose metabolism towards lactate production was observed under oxidative conditions. |
| format | Article |
| id | doaj-art-891bdcbfb6bd4667a0119dcdcafcb1ef |
| institution | Kabale University |
| issn | 1687-918X 1687-9198 |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-891bdcbfb6bd4667a0119dcdcafcb1ef2025-02-03T06:46:04ZengWileyInternational Journal of Microbiology1687-918X1687-91982010-01-01201010.1155/2010/896510896510Effect of Oxygen and Redox Potential on Glucose Fermentation in Thermotoga maritima under Controlled Physicochemical ConditionsRaja Lakhal0Richard Auria1Sylvain Davidson2Bernard Ollivier3Alain Dolla4Moktar Hamdi5Yannick Combet-Blanc6UMR D180, IRD, ESIL, Universités de Provence et de la Méditerranée, 163 Avenue de Luminy, Case 925, 13288 Marseille Cedex 09, FranceUMR D180, IRD, ESIL, Universités de Provence et de la Méditerranée, 163 Avenue de Luminy, Case 925, 13288 Marseille Cedex 09, FranceUMR D180, IRD, ESIL, Universités de Provence et de la Méditerranée, 163 Avenue de Luminy, Case 925, 13288 Marseille Cedex 09, FranceUMR D180, IRD, ESIL, Universités de Provence et de la Méditerranée, 163 Avenue de Luminy, Case 925, 13288 Marseille Cedex 09, FranceUnité Interactions et Modulateurs de Réponses, IFR88, CNRS, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, FranceLaboratoire d’Ecologie et de Technologie Microbienne, Institut National des Sciences Appliquées et de Technologies (INSAT), Université 7 Novembre Carthage, 2 Boulevard de la Terre, BP 676, 1080 Tunis, TunisiaUMR D180, IRD, ESIL, Universités de Provence et de la Méditerranée, 163 Avenue de Luminy, Case 925, 13288 Marseille Cedex 09, FranceBatch cultures of Thermotoga maritima were performed in a bioreactor equipped with instruments adapted for experiments performed at 80∘C to mimic the fluctuating oxidative conditions in the hot ecosystems it inhabits. When grown anaerobically on glucose, T. maritima was shown to significantly decrease the redox potential (Eh) of the culture medium down to about −480 mV, as long as glucose was available. Addition of oxygen into T. maritima cultures during the stationary growth phase led to a drastic reduction in glucose consumption rate. However, although oxygen was toxic, our experiment unambiguously proved that T. maritima was able to consume it during a 12-hour exposure period. Furthermore, a shift in glucose metabolism towards lactate production was observed under oxidative conditions.http://dx.doi.org/10.1155/2010/896510 |
| spellingShingle | Raja Lakhal Richard Auria Sylvain Davidson Bernard Ollivier Alain Dolla Moktar Hamdi Yannick Combet-Blanc Effect of Oxygen and Redox Potential on Glucose Fermentation in Thermotoga maritima under Controlled Physicochemical Conditions International Journal of Microbiology |
| title | Effect of Oxygen and Redox Potential on Glucose Fermentation in Thermotoga maritima under Controlled Physicochemical Conditions |
| title_full | Effect of Oxygen and Redox Potential on Glucose Fermentation in Thermotoga maritima under Controlled Physicochemical Conditions |
| title_fullStr | Effect of Oxygen and Redox Potential on Glucose Fermentation in Thermotoga maritima under Controlled Physicochemical Conditions |
| title_full_unstemmed | Effect of Oxygen and Redox Potential on Glucose Fermentation in Thermotoga maritima under Controlled Physicochemical Conditions |
| title_short | Effect of Oxygen and Redox Potential on Glucose Fermentation in Thermotoga maritima under Controlled Physicochemical Conditions |
| title_sort | effect of oxygen and redox potential on glucose fermentation in thermotoga maritima under controlled physicochemical conditions |
| url | http://dx.doi.org/10.1155/2010/896510 |
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