In Vivo and Impression Cytology Study on the Effect of Compatible Solutes Eye Drops on the Ocular Surface Epithelial Cell Quality in Dry Eye Patients
The aim of this study is to investigate in vivo and ex vivo ocular surface alterations induced by dry eye disease and modification after osmoprotective therapy. Forty-eight eyes of 24 patients suffering from dry eye have been recruited. All patients received Optive (compatible solutes) eye drops in...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2015/351424 |
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| author | Manuela Lanzini Claudia Curcio Rossella Annamaria Colabelli-Gisoldi Alessandra Mastropasqua Roberta Calienno Luca Agnifili Mario Nubile Leonardo Mastropasqua |
| author_facet | Manuela Lanzini Claudia Curcio Rossella Annamaria Colabelli-Gisoldi Alessandra Mastropasqua Roberta Calienno Luca Agnifili Mario Nubile Leonardo Mastropasqua |
| author_sort | Manuela Lanzini |
| collection | DOAJ |
| description | The aim of this study is to investigate in vivo and ex vivo ocular surface alterations induced by dry eye disease and modification after osmoprotective therapy. Forty-eight eyes of 24 patients suffering from dry eye have been recruited. All patients received Optive (compatible solutes) eye drops in one randomly selected eye and Hylogel (sodium hyaluronate 0,2%) in the other. Follow-up included a baseline visit and further examination 30-, 60-, and 90-day intervals (which comprises clinical evaluation, in vivo confocal microscopy—IVCM—of the ocular surface, and conjunctival impression cytology). No significant difference in Schirmer I Test, TBUT, and vital staining results was observed during the follow-up period in both groups. IVCM showed in all patients an improvement of ocular surface epithelial morphology and signs of inflammation (oedema and keratocyte activation). However, these modifications were more evident in patients treated with Optive therapy. A significant reduction of the expression of MMP9 and IL6 in Optive group was observed during the follow-up period in comparison to Hylogel treatment. Our results show that in dry eye disease therapy based on osmoprotective eye drops determines a reduction of inflammatory activation of ocular surface, with consequent improvement of the quality of corneal and conjunctival epithelium. |
| format | Article |
| id | doaj-art-88e990f197df4c4f8997fb9f2b1d185c |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-88e990f197df4c4f8997fb9f2b1d185c2025-02-03T01:27:40ZengWileyMediators of Inflammation0962-93511466-18612015-01-01201510.1155/2015/351424351424In Vivo and Impression Cytology Study on the Effect of Compatible Solutes Eye Drops on the Ocular Surface Epithelial Cell Quality in Dry Eye PatientsManuela Lanzini0Claudia Curcio1Rossella Annamaria Colabelli-Gisoldi2Alessandra Mastropasqua3Roberta Calienno4Luca Agnifili5Mario Nubile6Leonardo Mastropasqua7Department of Medicine and Ageing Sciences, Ophthalmology Clinic, University “G. d’Annunzio” of Chieti-Pescara, 66100 Chieti, ItalyDepartment of Medicine and Ageing Science, Biological Eye Center, CeSI, University “G. d’Annunzio” of Chieti-Pescara, 66100 Chieti, ItalyAzienda Ospedaliera San Giovanni-Addolorata, 00184 Roma, ItalyOphthalmology Clinic, Campus Biomedico, 00185 Rome, ItalyDepartment of Medicine and Ageing Sciences, Ophthalmology Clinic, University “G. d’Annunzio” of Chieti-Pescara, 66100 Chieti, ItalyDepartment of Medicine and Ageing Sciences, Ophthalmology Clinic, University “G. d’Annunzio” of Chieti-Pescara, 66100 Chieti, ItalyDepartment of Medicine and Ageing Sciences, Ophthalmology Clinic, University “G. d’Annunzio” of Chieti-Pescara, 66100 Chieti, ItalyDepartment of Medicine and Ageing Sciences, Ophthalmology Clinic, University “G. d’Annunzio” of Chieti-Pescara, 66100 Chieti, ItalyThe aim of this study is to investigate in vivo and ex vivo ocular surface alterations induced by dry eye disease and modification after osmoprotective therapy. Forty-eight eyes of 24 patients suffering from dry eye have been recruited. All patients received Optive (compatible solutes) eye drops in one randomly selected eye and Hylogel (sodium hyaluronate 0,2%) in the other. Follow-up included a baseline visit and further examination 30-, 60-, and 90-day intervals (which comprises clinical evaluation, in vivo confocal microscopy—IVCM—of the ocular surface, and conjunctival impression cytology). No significant difference in Schirmer I Test, TBUT, and vital staining results was observed during the follow-up period in both groups. IVCM showed in all patients an improvement of ocular surface epithelial morphology and signs of inflammation (oedema and keratocyte activation). However, these modifications were more evident in patients treated with Optive therapy. A significant reduction of the expression of MMP9 and IL6 in Optive group was observed during the follow-up period in comparison to Hylogel treatment. Our results show that in dry eye disease therapy based on osmoprotective eye drops determines a reduction of inflammatory activation of ocular surface, with consequent improvement of the quality of corneal and conjunctival epithelium.http://dx.doi.org/10.1155/2015/351424 |
| spellingShingle | Manuela Lanzini Claudia Curcio Rossella Annamaria Colabelli-Gisoldi Alessandra Mastropasqua Roberta Calienno Luca Agnifili Mario Nubile Leonardo Mastropasqua In Vivo and Impression Cytology Study on the Effect of Compatible Solutes Eye Drops on the Ocular Surface Epithelial Cell Quality in Dry Eye Patients Mediators of Inflammation |
| title | In Vivo and Impression Cytology Study on the Effect of Compatible Solutes Eye Drops on the Ocular Surface Epithelial Cell Quality in Dry Eye Patients |
| title_full | In Vivo and Impression Cytology Study on the Effect of Compatible Solutes Eye Drops on the Ocular Surface Epithelial Cell Quality in Dry Eye Patients |
| title_fullStr | In Vivo and Impression Cytology Study on the Effect of Compatible Solutes Eye Drops on the Ocular Surface Epithelial Cell Quality in Dry Eye Patients |
| title_full_unstemmed | In Vivo and Impression Cytology Study on the Effect of Compatible Solutes Eye Drops on the Ocular Surface Epithelial Cell Quality in Dry Eye Patients |
| title_short | In Vivo and Impression Cytology Study on the Effect of Compatible Solutes Eye Drops on the Ocular Surface Epithelial Cell Quality in Dry Eye Patients |
| title_sort | in vivo and impression cytology study on the effect of compatible solutes eye drops on the ocular surface epithelial cell quality in dry eye patients |
| url | http://dx.doi.org/10.1155/2015/351424 |
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