Effects of radiation and manganese oxide nanoparticles on human glioblastoma cell line U-87 MG glycolysis
Gliomas are the most common type of malignant brain tumors. Standard treatment of gliomas consists of surgical excision of the tumor with subsequent chemotherapy and radiotherapy. Tumor cells are characterized by rapid division with an increased uptake of glucose and its catabolism during glycolysis...
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Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2019-02-01
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| Series: | Вавиловский журнал генетики и селекции |
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| Online Access: | https://vavilov.elpub.ru/jour/article/view/1872 |
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| author | N. B. Illarionova D. V. Petrovski I. A. Razumov E. L. Zavyalov |
| author_facet | N. B. Illarionova D. V. Petrovski I. A. Razumov E. L. Zavyalov |
| author_sort | N. B. Illarionova |
| collection | DOAJ |
| description | Gliomas are the most common type of malignant brain tumors. Standard treatment of gliomas consists of surgical excision of the tumor with subsequent chemotherapy and radiotherapy. Tumor cells are characterized by rapid division with an increased uptake of glucose and its catabolism during glycolysis. To maintain rapid division, the level of glycolysis of the tumor cell is significantly increased, compared with normal cells. It is known that some nanoparticles (NP) have the property of accumulating in tumors. In particular, NPs of manganese oxide can penetrate into the brain and, with considerable accumulation, cause toxic effects. These facts served as a prerequisite for studying the effects of manganese oxide NPs on the viability of glioma cells. The purpose of this work was to study the effects of manganese oxide NPs, as well as their combination with gamma irradiation on the glycolysis of glioma cells. The cells were irradiated using the research radiobiological gamma-installation IGUR-1 based on 137Cs. The level of cell glycolysis was determined using the standard glycolytic stress test on a Seahorse XFp platform. Cell viability was determined using the ViaCount reagent staining of living and dead cells. Their count was performed using flow cytometry. We showed that the glycolysis of U-87 MG glioma cells was significantly reduced when incubated for 48 hours with manganese oxide NPs. Irradiation in combination with NPs or alone did not have significant effects on glycolysis of gliomas. Glioma incubation with manganese oxide NPs for 72 hours led to a significant reduction in cell viability. This study may be useful for the development of new therapies and diagnosis of gliomas. |
| format | Article |
| id | doaj-art-88e85ed1c12541fb98879ad3badf0670 |
| institution | Kabale University |
| issn | 2500-3259 |
| language | English |
| publishDate | 2019-02-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj-art-88e85ed1c12541fb98879ad3badf06702025-02-01T09:58:07ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592019-02-01231818510.18699/VJ19.465875Effects of radiation and manganese oxide nanoparticles on human glioblastoma cell line U-87 MG glycolysisN. B. Illarionova0D. V. Petrovski1I. A. Razumov2E. L. Zavyalov3Institute of Cytology and Genetics, SB RASInstitute of Cytology and Genetics, SB RASInstitute of Cytology and Genetics, SB RASInstitute of Cytology and Genetics, SB RAS; Novosibirsk State UniversityGliomas are the most common type of malignant brain tumors. Standard treatment of gliomas consists of surgical excision of the tumor with subsequent chemotherapy and radiotherapy. Tumor cells are characterized by rapid division with an increased uptake of glucose and its catabolism during glycolysis. To maintain rapid division, the level of glycolysis of the tumor cell is significantly increased, compared with normal cells. It is known that some nanoparticles (NP) have the property of accumulating in tumors. In particular, NPs of manganese oxide can penetrate into the brain and, with considerable accumulation, cause toxic effects. These facts served as a prerequisite for studying the effects of manganese oxide NPs on the viability of glioma cells. The purpose of this work was to study the effects of manganese oxide NPs, as well as their combination with gamma irradiation on the glycolysis of glioma cells. The cells were irradiated using the research radiobiological gamma-installation IGUR-1 based on 137Cs. The level of cell glycolysis was determined using the standard glycolytic stress test on a Seahorse XFp platform. Cell viability was determined using the ViaCount reagent staining of living and dead cells. Their count was performed using flow cytometry. We showed that the glycolysis of U-87 MG glioma cells was significantly reduced when incubated for 48 hours with manganese oxide NPs. Irradiation in combination with NPs or alone did not have significant effects on glycolysis of gliomas. Glioma incubation with manganese oxide NPs for 72 hours led to a significant reduction in cell viability. This study may be useful for the development of new therapies and diagnosis of gliomas.https://vavilov.elpub.ru/jour/article/view/1872gliomananoparticlesmanganese oxideglycolysis |
| spellingShingle | N. B. Illarionova D. V. Petrovski I. A. Razumov E. L. Zavyalov Effects of radiation and manganese oxide nanoparticles on human glioblastoma cell line U-87 MG glycolysis Вавиловский журнал генетики и селекции glioma nanoparticles manganese oxide glycolysis |
| title | Effects of radiation and manganese oxide nanoparticles on human glioblastoma cell line U-87 MG glycolysis |
| title_full | Effects of radiation and manganese oxide nanoparticles on human glioblastoma cell line U-87 MG glycolysis |
| title_fullStr | Effects of radiation and manganese oxide nanoparticles on human glioblastoma cell line U-87 MG glycolysis |
| title_full_unstemmed | Effects of radiation and manganese oxide nanoparticles on human glioblastoma cell line U-87 MG glycolysis |
| title_short | Effects of radiation and manganese oxide nanoparticles on human glioblastoma cell line U-87 MG glycolysis |
| title_sort | effects of radiation and manganese oxide nanoparticles on human glioblastoma cell line u 87 mg glycolysis |
| topic | glioma nanoparticles manganese oxide glycolysis |
| url | https://vavilov.elpub.ru/jour/article/view/1872 |
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