Role of myostatin promoter mutations in growth and molting regulation of Macrobrachium rosenbergii
Myostatin (Mstn), a negative regulatory factor in myocytes, has garnered significant attention. This study focused on the Mstn gene and its 24-base-pair mutant in the promoter region of the giant freshwater prawn (Macrobrachium rosenbergii), aiming to investigate the regulatory roles of Mstn and its...
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Frontiers Media S.A.
2025-05-01
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| Series: | Frontiers in Marine Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmars.2025.1565567/full |
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| author | Qiyao Su Qiyao Su Jie Wei Yakun Wang Bai Liufu Bai Liufu Huarong Li Huarong Li Zhuang Mai Zhuang Mai Kunhao Hong Kunhao Hong Qiaoyan Zhou Tianhui Jiao Tianhui Jiao Xidong Mu Lingyun Yu |
| author_facet | Qiyao Su Qiyao Su Jie Wei Yakun Wang Bai Liufu Bai Liufu Huarong Li Huarong Li Zhuang Mai Zhuang Mai Kunhao Hong Kunhao Hong Qiaoyan Zhou Tianhui Jiao Tianhui Jiao Xidong Mu Lingyun Yu |
| author_sort | Qiyao Su |
| collection | DOAJ |
| description | Myostatin (Mstn), a negative regulatory factor in myocytes, has garnered significant attention. This study focused on the Mstn gene and its 24-base-pair mutant in the promoter region of the giant freshwater prawn (Macrobrachium rosenbergii), aiming to investigate the regulatory roles of Mstn and its mutant in M. rosenbergii. The research provides theoretical insights into the functional mechanisms of Mstn in crustaceans. Spatiotemporal expression patterns revealed Mstn presence throughout embryonic development and in various body tissues, with peak expression during the gastrula stage and in the hepatopancreas. During the molting cycle, Mstn expression levels decreased in the order of post-molt (A phase), pre-molt (D3 phase), and molting (E phase). Knockdown experiments targeting two genotypes within the Mstn promoter region significantly reduced growth rates and extended molting cycles compared to control group. Further trials on the F2 generation confirmed these findings, highlighting that promoter knockdown influenced the expression of three molting-related genes and slowed the growth rate of M. rosenbergii. This study clarifies the functional role of Mstn in crustacean growth and molting, providing a foundation for understanding its regulatory mechanisms and offering potential applications in aquaculture. |
| format | Article |
| id | doaj-art-88e7dba0ac834a4e8740767b79d5dac7 |
| institution | DOAJ |
| issn | 2296-7745 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Marine Science |
| spelling | doaj-art-88e7dba0ac834a4e8740767b79d5dac72025-08-20T03:07:16ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452025-05-011210.3389/fmars.2025.15655671565567Role of myostatin promoter mutations in growth and molting regulation of Macrobrachium rosenbergiiQiyao Su0Qiyao Su1Jie Wei2Yakun Wang3Bai Liufu4Bai Liufu5Huarong Li6Huarong Li7Zhuang Mai8Zhuang Mai9Kunhao Hong10Kunhao Hong11Qiaoyan Zhou12Tianhui Jiao13Tianhui Jiao14Xidong Mu15Lingyun Yu16Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation of Ministry of Agriculture and Rural Affairs, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, ChinaCollege of Fisheries and Life Science, Shanghai Ocean University, Shanghai, ChinaKey Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation of Ministry of Agriculture and Rural Affairs, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, ChinaKey Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation of Ministry of Agriculture and Rural Affairs, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, ChinaKey Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation of Ministry of Agriculture and Rural Affairs, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, ChinaSchool of Fishery, Zhejiang Ocean University, Zhoushan, ChinaKey Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation of Ministry of Agriculture and Rural Affairs, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, ChinaCollege of Fisheries and Life Science, Shanghai Ocean University, Shanghai, ChinaKey Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation of Ministry of Agriculture and Rural Affairs, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, ChinaSchool of Fishery, Zhejiang Ocean University, Zhoushan, ChinaKey Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation of Ministry of Agriculture and Rural Affairs, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, ChinaCollege of Fisheries and Life Science, Shanghai Ocean University, Shanghai, ChinaKey Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation of Ministry of Agriculture and Rural Affairs, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, ChinaKey Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation of Ministry of Agriculture and Rural Affairs, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, ChinaCollege of Fisheries and Life Science, Shanghai Ocean University, Shanghai, ChinaKey Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation of Ministry of Agriculture and Rural Affairs, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, ChinaKey Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation of Ministry of Agriculture and Rural Affairs, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, ChinaMyostatin (Mstn), a negative regulatory factor in myocytes, has garnered significant attention. This study focused on the Mstn gene and its 24-base-pair mutant in the promoter region of the giant freshwater prawn (Macrobrachium rosenbergii), aiming to investigate the regulatory roles of Mstn and its mutant in M. rosenbergii. The research provides theoretical insights into the functional mechanisms of Mstn in crustaceans. Spatiotemporal expression patterns revealed Mstn presence throughout embryonic development and in various body tissues, with peak expression during the gastrula stage and in the hepatopancreas. During the molting cycle, Mstn expression levels decreased in the order of post-molt (A phase), pre-molt (D3 phase), and molting (E phase). Knockdown experiments targeting two genotypes within the Mstn promoter region significantly reduced growth rates and extended molting cycles compared to control group. Further trials on the F2 generation confirmed these findings, highlighting that promoter knockdown influenced the expression of three molting-related genes and slowed the growth rate of M. rosenbergii. This study clarifies the functional role of Mstn in crustacean growth and molting, providing a foundation for understanding its regulatory mechanisms and offering potential applications in aquaculture.https://www.frontiersin.org/articles/10.3389/fmars.2025.1565567/fullMacrobrachium rosenbergiimyostatinRNAimoltgrowth |
| spellingShingle | Qiyao Su Qiyao Su Jie Wei Yakun Wang Bai Liufu Bai Liufu Huarong Li Huarong Li Zhuang Mai Zhuang Mai Kunhao Hong Kunhao Hong Qiaoyan Zhou Tianhui Jiao Tianhui Jiao Xidong Mu Lingyun Yu Role of myostatin promoter mutations in growth and molting regulation of Macrobrachium rosenbergii Frontiers in Marine Science Macrobrachium rosenbergii myostatin RNAi molt growth |
| title | Role of myostatin promoter mutations in growth and molting regulation of Macrobrachium rosenbergii |
| title_full | Role of myostatin promoter mutations in growth and molting regulation of Macrobrachium rosenbergii |
| title_fullStr | Role of myostatin promoter mutations in growth and molting regulation of Macrobrachium rosenbergii |
| title_full_unstemmed | Role of myostatin promoter mutations in growth and molting regulation of Macrobrachium rosenbergii |
| title_short | Role of myostatin promoter mutations in growth and molting regulation of Macrobrachium rosenbergii |
| title_sort | role of myostatin promoter mutations in growth and molting regulation of macrobrachium rosenbergii |
| topic | Macrobrachium rosenbergii myostatin RNAi molt growth |
| url | https://www.frontiersin.org/articles/10.3389/fmars.2025.1565567/full |
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