Quantitative Determination of 18-β-Glycyrrhetinic Acid in HepG2 Cell Line by High Performance Liquid Chromatography Method
A reverse phase high performance liquid chromatographic (RP-HPLC) method was developed for identification and estimation of 18-β-glycyrrhetinic acid (GA) in HepG2 cell line. The analysis was carried out using a JASCO HPLC system with a C-18 (3 μm) Supelco reversed phase column (150 x 4.7 mm) using a...
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2018-01-01
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| Series: | International Journal of Analytical Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2018/5673186 |
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| author | Giuseppina Nocca Cinzia Callà Stefano Angelo Santini Adriana Amalfitano Luca Marigo Diana Valeria Rossetti Gianrico Spagnuolo Massimo Cordaro |
| author_facet | Giuseppina Nocca Cinzia Callà Stefano Angelo Santini Adriana Amalfitano Luca Marigo Diana Valeria Rossetti Gianrico Spagnuolo Massimo Cordaro |
| author_sort | Giuseppina Nocca |
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| description | A reverse phase high performance liquid chromatographic (RP-HPLC) method was developed for identification and estimation of 18-β-glycyrrhetinic acid (GA) in HepG2 cell line. The analysis was carried out using a JASCO HPLC system with a C-18 (3 μm) Supelco reversed phase column (150 x 4.7 mm) using a mobile phase of 80% CH3OH and 20% of CH3CN: tetrahydrofuran: water (10:80:10, v/v/v). The method was linear in the concentration range of 1.5–120 μg /mL (n = 5). The LOD and LOQ were determined based on standard deviation of the y-intercept and the slope of the calibration curve. The LOD and LOQ values were found to be 11.46 μg/mL and 34.72 μg/mL, respectively. The mean percentage recovery by standard addition experiments of GA is 92.4 % ± 5.2%. The intracellular GA concentration value, obtained as mean of five different determinations, was 45.8 ± 7.45 μg/mL. We have developed a HPLC-UV method for quantitative determination of GA inside cells, with advantages in the cost reduction and economy of the analytical process. |
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| id | doaj-art-88a7b233f6fe44f78d7208550290259d |
| institution | Kabale University |
| issn | 1687-8760 1687-8779 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
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| series | International Journal of Analytical Chemistry |
| spelling | doaj-art-88a7b233f6fe44f78d7208550290259d2025-02-03T05:44:10ZengWileyInternational Journal of Analytical Chemistry1687-87601687-87792018-01-01201810.1155/2018/56731865673186Quantitative Determination of 18-β-Glycyrrhetinic Acid in HepG2 Cell Line by High Performance Liquid Chromatography MethodGiuseppina Nocca0Cinzia Callà1Stefano Angelo Santini2Adriana Amalfitano3Luca Marigo4Diana Valeria Rossetti5Gianrico Spagnuolo6Massimo Cordaro7Istituto di Biochimica e Biochimica Clinica, Università Cattolica del Sacro Cuore Rome, ItalyUOC Chimica, Biochimica e Biologia Molecolare, Dip. Scienze di Laboratorio e Infettivologiche, Fondazione Policlinico Universitario A. Gemelli, IRCCS, Università Cattolica del Sacro Cuore Rome, ItalyUOC Chimica, Biochimica e Biologia Molecolare, Dip. Scienze di Laboratorio e Infettivologiche, Fondazione Policlinico Universitario A. Gemelli, IRCCS, Università Cattolica del Sacro Cuore Rome, ItalyIstituto di Biochimica e Biochimica Clinica, Università Cattolica del Sacro Cuore Rome, ItalyUOC Odontoiatria Generale e Ortodonzia, Dip Scienze dell’Invecchiamento, Neurologiche, Ortopediche e della Testa Collo. Fondazione Policlinico Universitario A. Gemelli, IRCCS, Università Cattolica del Sacro Cuore Rome, ItalyIstituto di Biochimica e Biochimica Clinica, Università Cattolica del Sacro Cuore Rome, ItalyDepartment of Neurosciences, Reproductive and Odontostomatological Sciences University of Naples “Federico II”, ItalyUOC Odontoiatria Generale e Ortodonzia, Dip Scienze dell’Invecchiamento, Neurologiche, Ortopediche e della Testa Collo. Fondazione Policlinico Universitario A. Gemelli, IRCCS, Università Cattolica del Sacro Cuore Rome, ItalyA reverse phase high performance liquid chromatographic (RP-HPLC) method was developed for identification and estimation of 18-β-glycyrrhetinic acid (GA) in HepG2 cell line. The analysis was carried out using a JASCO HPLC system with a C-18 (3 μm) Supelco reversed phase column (150 x 4.7 mm) using a mobile phase of 80% CH3OH and 20% of CH3CN: tetrahydrofuran: water (10:80:10, v/v/v). The method was linear in the concentration range of 1.5–120 μg /mL (n = 5). The LOD and LOQ were determined based on standard deviation of the y-intercept and the slope of the calibration curve. The LOD and LOQ values were found to be 11.46 μg/mL and 34.72 μg/mL, respectively. The mean percentage recovery by standard addition experiments of GA is 92.4 % ± 5.2%. The intracellular GA concentration value, obtained as mean of five different determinations, was 45.8 ± 7.45 μg/mL. We have developed a HPLC-UV method for quantitative determination of GA inside cells, with advantages in the cost reduction and economy of the analytical process.http://dx.doi.org/10.1155/2018/5673186 |
| spellingShingle | Giuseppina Nocca Cinzia Callà Stefano Angelo Santini Adriana Amalfitano Luca Marigo Diana Valeria Rossetti Gianrico Spagnuolo Massimo Cordaro Quantitative Determination of 18-β-Glycyrrhetinic Acid in HepG2 Cell Line by High Performance Liquid Chromatography Method International Journal of Analytical Chemistry |
| title | Quantitative Determination of 18-β-Glycyrrhetinic Acid in HepG2 Cell Line by High Performance Liquid Chromatography Method |
| title_full | Quantitative Determination of 18-β-Glycyrrhetinic Acid in HepG2 Cell Line by High Performance Liquid Chromatography Method |
| title_fullStr | Quantitative Determination of 18-β-Glycyrrhetinic Acid in HepG2 Cell Line by High Performance Liquid Chromatography Method |
| title_full_unstemmed | Quantitative Determination of 18-β-Glycyrrhetinic Acid in HepG2 Cell Line by High Performance Liquid Chromatography Method |
| title_short | Quantitative Determination of 18-β-Glycyrrhetinic Acid in HepG2 Cell Line by High Performance Liquid Chromatography Method |
| title_sort | quantitative determination of 18 β glycyrrhetinic acid in hepg2 cell line by high performance liquid chromatography method |
| url | http://dx.doi.org/10.1155/2018/5673186 |
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