Evaluation of nitrous oxide reduction in solid carbon source-driven counter-diffusional biofilm denitrification system
Solid carbon-driven biofilm system can provide sufficient carbon source for denitrification, while its counter-diffusional structure could inevitably induce the delayed carbon-nitrogen contact and electron transport, further affecting carbon footprints mainly contributed by nitrous oxide (N2O) at wa...
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Elsevier
2025-05-01
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| Series: | Water Research X |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589914725000064 |
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| author | Yingrui Liu Feng Chen Yanying He Yufen Wang Tingting Zhu Yindong Tong Yingxin Zhao Bing-Jie Ni Yiwen Liu |
| author_facet | Yingrui Liu Feng Chen Yanying He Yufen Wang Tingting Zhu Yindong Tong Yingxin Zhao Bing-Jie Ni Yiwen Liu |
| author_sort | Yingrui Liu |
| collection | DOAJ |
| description | Solid carbon-driven biofilm system can provide sufficient carbon source for denitrification, while its counter-diffusional structure could inevitably induce the delayed carbon-nitrogen contact and electron transport, further affecting carbon footprints mainly contributed by nitrous oxide (N2O) at wastewater treatment plants (WWTPs). However, the detailed understanding of N2O dynamics during solid-phase denitrification (SPD) has not been disclosed. In this work, a fixed bed bioreactor driven by polycaprolactone (PCL) was constructed and operated over 180 days, achieving 97 %-99 % of total nitrogen (TN) removal efficiency. Biochemical results indicated that under the condition that each nitrogen oxide (NOx) concentration was maintained at 30 mg-N/L, the electron competition between upstream and downstream electron pools was still observed during PCL-driven denitrification even providing sufficient carbon source. For example, under the coexistent nitrate (NO3-)+ nitrite (NO2-)+N2O condition, few electrons (i.e., 12.6 %) distributed to N2O reductase (Nos), significantly decreasing the N2O reduction rate (i.e., 1.42 mg/g VSS/h). Under the condition that TN concentration was maintained at 30 mg-N/L, the TN removal rate in the scheme containing NO3-+NO2-+N2O was observed to be 1.75–2.3 times higher than that of the scheme with sole NOx of 30 mg-N/L. This suggested that when treating wastewater containing multiple NOx, the PCL-driven biofilm denitrification system can not only relatively improve the total nitrogen removal efficiency, but also relatively alleviate N2O emissions. The higher abundance of Bacteroidota and Comamonadaceae ensured the stable carbon source release and nitrogen conversion states. |
| format | Article |
| id | doaj-art-889ae5a46e69412f9559644c77b20196 |
| institution | Kabale University |
| issn | 2589-9147 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Water Research X |
| spelling | doaj-art-889ae5a46e69412f9559644c77b201962025-01-27T04:22:08ZengElsevierWater Research X2589-91472025-05-0127100306Evaluation of nitrous oxide reduction in solid carbon source-driven counter-diffusional biofilm denitrification systemYingrui Liu0Feng Chen1Yanying He2Yufen Wang3Tingting Zhu4Yindong Tong5Yingxin Zhao6Bing-Jie Ni7Yiwen Liu8School of Environmental Science and Engineering, Tianjin University, Tianjin 300072, PR ChinaSchool of Environmental Science and Engineering, Tianjin University, Tianjin 300072, PR ChinaSchool of Environmental Science and Engineering, Tianjin University, Tianjin 300072, PR ChinaSchool of Environmental Science and Engineering, Tianjin University, Tianjin 300072, PR ChinaSchool of Environmental Science and Engineering, Tianjin University, Tianjin 300072, PR China; Corresponding authors.School of Environmental Science and Engineering, Tianjin University, Tianjin 300072, PR ChinaSchool of Environmental Science and Engineering, Tianjin University, Tianjin 300072, PR ChinaSchool of Civil and Environmental Engineering, University of New South Wales, Sydney, New South Wales, 2052, AustraliaSchool of Environmental Science and Engineering, Tianjin University, Tianjin 300072, PR China; Corresponding authors.Solid carbon-driven biofilm system can provide sufficient carbon source for denitrification, while its counter-diffusional structure could inevitably induce the delayed carbon-nitrogen contact and electron transport, further affecting carbon footprints mainly contributed by nitrous oxide (N2O) at wastewater treatment plants (WWTPs). However, the detailed understanding of N2O dynamics during solid-phase denitrification (SPD) has not been disclosed. In this work, a fixed bed bioreactor driven by polycaprolactone (PCL) was constructed and operated over 180 days, achieving 97 %-99 % of total nitrogen (TN) removal efficiency. Biochemical results indicated that under the condition that each nitrogen oxide (NOx) concentration was maintained at 30 mg-N/L, the electron competition between upstream and downstream electron pools was still observed during PCL-driven denitrification even providing sufficient carbon source. For example, under the coexistent nitrate (NO3-)+ nitrite (NO2-)+N2O condition, few electrons (i.e., 12.6 %) distributed to N2O reductase (Nos), significantly decreasing the N2O reduction rate (i.e., 1.42 mg/g VSS/h). Under the condition that TN concentration was maintained at 30 mg-N/L, the TN removal rate in the scheme containing NO3-+NO2-+N2O was observed to be 1.75–2.3 times higher than that of the scheme with sole NOx of 30 mg-N/L. This suggested that when treating wastewater containing multiple NOx, the PCL-driven biofilm denitrification system can not only relatively improve the total nitrogen removal efficiency, but also relatively alleviate N2O emissions. The higher abundance of Bacteroidota and Comamonadaceae ensured the stable carbon source release and nitrogen conversion states.http://www.sciencedirect.com/science/article/pii/S2589914725000064Nitrous oxide (N2O)Polycaprolactone (PCL)Counter-diffusional biofilmSolid carbon sourceSolid-phase denitrification (SPD) |
| spellingShingle | Yingrui Liu Feng Chen Yanying He Yufen Wang Tingting Zhu Yindong Tong Yingxin Zhao Bing-Jie Ni Yiwen Liu Evaluation of nitrous oxide reduction in solid carbon source-driven counter-diffusional biofilm denitrification system Water Research X Nitrous oxide (N2O) Polycaprolactone (PCL) Counter-diffusional biofilm Solid carbon source Solid-phase denitrification (SPD) |
| title | Evaluation of nitrous oxide reduction in solid carbon source-driven counter-diffusional biofilm denitrification system |
| title_full | Evaluation of nitrous oxide reduction in solid carbon source-driven counter-diffusional biofilm denitrification system |
| title_fullStr | Evaluation of nitrous oxide reduction in solid carbon source-driven counter-diffusional biofilm denitrification system |
| title_full_unstemmed | Evaluation of nitrous oxide reduction in solid carbon source-driven counter-diffusional biofilm denitrification system |
| title_short | Evaluation of nitrous oxide reduction in solid carbon source-driven counter-diffusional biofilm denitrification system |
| title_sort | evaluation of nitrous oxide reduction in solid carbon source driven counter diffusional biofilm denitrification system |
| topic | Nitrous oxide (N2O) Polycaprolactone (PCL) Counter-diffusional biofilm Solid carbon source Solid-phase denitrification (SPD) |
| url | http://www.sciencedirect.com/science/article/pii/S2589914725000064 |
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