Metabolism Analysis of Alantolactone and Isoalantolactone in Rats by Oral Administration
Alantolactone and isoalantolactone are the major active ingredients of Inulae Radix. Their metabolism in vivo and in vitro was investigated by UPLC-Q-TOF-MS for the first time. As a result, nine metabolites in vivo including cysteine conjugates, oxidates, dehydrogenates, and hydrates were detected i...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2018/2026357 |
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| author | Mengyue Wang Renjie Xu Ying Peng Xiaobo Li |
| author_facet | Mengyue Wang Renjie Xu Ying Peng Xiaobo Li |
| author_sort | Mengyue Wang |
| collection | DOAJ |
| description | Alantolactone and isoalantolactone are the major active ingredients of Inulae Radix. Their metabolism in vivo and in vitro was investigated by UPLC-Q-TOF-MS for the first time. As a result, nine metabolites in vivo including cysteine conjugates, oxidates, dehydrogenates, and hydrates were detected in rat bile after oral administration. The metabolites produced in vitro by incubation with rat liver microsomes were found to be substantially identical to those detected in vivo. However, no metabolites were detected in the samples of plasma, feces, and urine or in the incubates of gastric juice, intestinal juice, and intestinal bacteria. These results reveal that the liver is the main metabolic organ for alantolactone and isoalantolactone, and the first pass effect of the liver appears to be the reason for the low oral bioavailability of the two lactones. |
| format | Article |
| id | doaj-art-8883096129d94a59ba86a75e89b94cc2 |
| institution | Kabale University |
| issn | 2090-9063 2090-9071 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Chemistry |
| spelling | doaj-art-8883096129d94a59ba86a75e89b94cc22025-02-03T06:13:50ZengWileyJournal of Chemistry2090-90632090-90712018-01-01201810.1155/2018/20263572026357Metabolism Analysis of Alantolactone and Isoalantolactone in Rats by Oral AdministrationMengyue Wang0Renjie Xu1Ying Peng2Xiaobo Li3School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, ChinaSchool of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, ChinaSchool of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, ChinaSchool of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, ChinaAlantolactone and isoalantolactone are the major active ingredients of Inulae Radix. Their metabolism in vivo and in vitro was investigated by UPLC-Q-TOF-MS for the first time. As a result, nine metabolites in vivo including cysteine conjugates, oxidates, dehydrogenates, and hydrates were detected in rat bile after oral administration. The metabolites produced in vitro by incubation with rat liver microsomes were found to be substantially identical to those detected in vivo. However, no metabolites were detected in the samples of plasma, feces, and urine or in the incubates of gastric juice, intestinal juice, and intestinal bacteria. These results reveal that the liver is the main metabolic organ for alantolactone and isoalantolactone, and the first pass effect of the liver appears to be the reason for the low oral bioavailability of the two lactones.http://dx.doi.org/10.1155/2018/2026357 |
| spellingShingle | Mengyue Wang Renjie Xu Ying Peng Xiaobo Li Metabolism Analysis of Alantolactone and Isoalantolactone in Rats by Oral Administration Journal of Chemistry |
| title | Metabolism Analysis of Alantolactone and Isoalantolactone in Rats by Oral Administration |
| title_full | Metabolism Analysis of Alantolactone and Isoalantolactone in Rats by Oral Administration |
| title_fullStr | Metabolism Analysis of Alantolactone and Isoalantolactone in Rats by Oral Administration |
| title_full_unstemmed | Metabolism Analysis of Alantolactone and Isoalantolactone in Rats by Oral Administration |
| title_short | Metabolism Analysis of Alantolactone and Isoalantolactone in Rats by Oral Administration |
| title_sort | metabolism analysis of alantolactone and isoalantolactone in rats by oral administration |
| url | http://dx.doi.org/10.1155/2018/2026357 |
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