Immobilization of Laccase in Alginate-Gelatin Mixed Gel and Decolorization of Synthetic Dyes
Alginate-gelatin mixed gel was applied to immobilized laccase for decolorization of some synthetic dyes including crystal violet. The immobilization procedure was accomplished by adding alginate to a gelatin solution containing the enzyme and the subsequent dropwise addition of the mixture into a st...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Bioinorganic Chemistry and Applications |
| Online Access: | http://dx.doi.org/10.1155/2012/823830 |
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| author | Mehdi Mogharabi Nasser Nassiri-Koopaei Maryam Bozorgi-Koushalshahi Nastaran Nafissi-Varcheh Ghodsieh Bagherzadeh Mohammad Ali Faramarzi |
| author_facet | Mehdi Mogharabi Nasser Nassiri-Koopaei Maryam Bozorgi-Koushalshahi Nastaran Nafissi-Varcheh Ghodsieh Bagherzadeh Mohammad Ali Faramarzi |
| author_sort | Mehdi Mogharabi |
| collection | DOAJ |
| description | Alginate-gelatin mixed gel was applied to immobilized laccase for decolorization of some synthetic dyes including crystal violet. The immobilization procedure was accomplished by adding alginate to a gelatin solution containing the enzyme and the subsequent dropwise addition of the mixture into a stirred CaCl2 solution. The obtained data showed that both immobilized and free enzymes acted optimally at 50°C for removal of crystal violet, but the entrapped enzyme showed higher thermal stability compared to the free enzyme. The immobilized enzyme represented optimum decolorization at pH 8. Reusability of the entrapped laccase was also studied and the results showed that ca. 85% activity was retained after five successive cycles. The best removal condition was applied for decolorization of seven other synthetic dyes. Results showed that the maximum and minimum dye removal was related to amido black 10B and eosin, respectively. |
| format | Article |
| id | doaj-art-887f55e8f72b4667be765b552f32afe9 |
| institution | Kabale University |
| issn | 1565-3633 1687-479X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Bioinorganic Chemistry and Applications |
| spelling | doaj-art-887f55e8f72b4667be765b552f32afe92025-02-03T01:25:20ZengWileyBioinorganic Chemistry and Applications1565-36331687-479X2012-01-01201210.1155/2012/823830823830Immobilization of Laccase in Alginate-Gelatin Mixed Gel and Decolorization of Synthetic DyesMehdi Mogharabi0Nasser Nassiri-Koopaei1Maryam Bozorgi-Koushalshahi2Nastaran Nafissi-Varcheh3Ghodsieh Bagherzadeh4Mohammad Ali Faramarzi5Department of Pharmaceutical Biotechnology, Faculty of Pharmacy and Biotechnology Research Center, Tehran University of Medical Sciences, P.O. Box 14155–6451, Tehran 14174, IranDepartment of Pharmaceutical Biotechnology, Faculty of Pharmacy and Biotechnology Research Center, Tehran University of Medical Sciences, P.O. Box 14155–6451, Tehran 14174, IranDepartment of Pharmaceutical Biotechnology, Faculty of Pharmacy and Biotechnology Research Center, Tehran University of Medical Sciences, P.O. Box 14155–6451, Tehran 14174, IranDepartment of Pharmaceutical Biotechnology, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran 1457795343, IranDepartment of Chemistry, Faculty of Sciences, University of Birjand, Birjand 9717853577, IranDepartment of Pharmaceutical Biotechnology, Faculty of Pharmacy and Biotechnology Research Center, Tehran University of Medical Sciences, P.O. Box 14155–6451, Tehran 14174, IranAlginate-gelatin mixed gel was applied to immobilized laccase for decolorization of some synthetic dyes including crystal violet. The immobilization procedure was accomplished by adding alginate to a gelatin solution containing the enzyme and the subsequent dropwise addition of the mixture into a stirred CaCl2 solution. The obtained data showed that both immobilized and free enzymes acted optimally at 50°C for removal of crystal violet, but the entrapped enzyme showed higher thermal stability compared to the free enzyme. The immobilized enzyme represented optimum decolorization at pH 8. Reusability of the entrapped laccase was also studied and the results showed that ca. 85% activity was retained after five successive cycles. The best removal condition was applied for decolorization of seven other synthetic dyes. Results showed that the maximum and minimum dye removal was related to amido black 10B and eosin, respectively.http://dx.doi.org/10.1155/2012/823830 |
| spellingShingle | Mehdi Mogharabi Nasser Nassiri-Koopaei Maryam Bozorgi-Koushalshahi Nastaran Nafissi-Varcheh Ghodsieh Bagherzadeh Mohammad Ali Faramarzi Immobilization of Laccase in Alginate-Gelatin Mixed Gel and Decolorization of Synthetic Dyes Bioinorganic Chemistry and Applications |
| title | Immobilization of Laccase in Alginate-Gelatin Mixed Gel and Decolorization of Synthetic Dyes |
| title_full | Immobilization of Laccase in Alginate-Gelatin Mixed Gel and Decolorization of Synthetic Dyes |
| title_fullStr | Immobilization of Laccase in Alginate-Gelatin Mixed Gel and Decolorization of Synthetic Dyes |
| title_full_unstemmed | Immobilization of Laccase in Alginate-Gelatin Mixed Gel and Decolorization of Synthetic Dyes |
| title_short | Immobilization of Laccase in Alginate-Gelatin Mixed Gel and Decolorization of Synthetic Dyes |
| title_sort | immobilization of laccase in alginate gelatin mixed gel and decolorization of synthetic dyes |
| url | http://dx.doi.org/10.1155/2012/823830 |
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