Development of detection system for lead ions in mixture solutions using UV-Vis measurements with peptide immobilized microbeads
Abstract Environmental pollution caused by heavy metals are problems worldwide. In particular, pollution and poisoning by lead ions (Pb2+) continue to be common and serious problems. Hence, there is a need for a widely usable method to easily detect Pb2+ from solutions containing organic materials f...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-01-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-86082-z |
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| author | Shuhei Yoshida Koki Yoshida Yoshio Hamada Takaaki Tsuruoka Kenji Usui |
| author_facet | Shuhei Yoshida Koki Yoshida Yoshio Hamada Takaaki Tsuruoka Kenji Usui |
| author_sort | Shuhei Yoshida |
| collection | DOAJ |
| description | Abstract Environmental pollution caused by heavy metals are problems worldwide. In particular, pollution and poisoning by lead ions (Pb2+) continue to be common and serious problems. Hence, there is a need for a widely usable method to easily detect Pb2+ from solutions containing organic materials from environmental water such as seas, ponds, etc. Here, we established a system to easily detect Pb2+ from such mixture solutions using Pb2+ binding peptide immobilized beads (peptidyl beads) and ultraviolet (UV) absorption measurements. This method could detect Pb2+ at low concentrations equivalent to inductively coupled plasmon-atomic emission spectroscopy (ICP-AES). Using the detected values to create a calibration curve, it was found that there was a positive correlation between the concentration of Pb2+ and absorbance, which also made it possible to quantify sub-µM Pb2+ in the solutions. Furthermore, Pb2+ was detected and quantified under mixed conditions of environmental water such as seas, rivers, and ponds. This method is expected to become a versatile and easy-to-use Pb2+ detection method for end-users worldwide. |
| format | Article |
| id | doaj-art-887a4df3c6a248cb8211bcd769e6b0b2 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-887a4df3c6a248cb8211bcd769e6b0b22025-01-26T12:34:15ZengNature PortfolioScientific Reports2045-23222025-01-011511910.1038/s41598-025-86082-zDevelopment of detection system for lead ions in mixture solutions using UV-Vis measurements with peptide immobilized microbeadsShuhei Yoshida0Koki Yoshida1Yoshio Hamada2Takaaki Tsuruoka3Kenji Usui4Faculty of Frontiers of Innovative Research in Science and Technology (FIRST), Konan UniversityFaculty of Frontiers of Innovative Research in Science and Technology (FIRST), Konan UniversityFaculty of Frontiers of Innovative Research in Science and Technology (FIRST), Konan UniversityFaculty of Frontiers of Innovative Research in Science and Technology (FIRST), Konan UniversityFaculty of Frontiers of Innovative Research in Science and Technology (FIRST), Konan UniversityAbstract Environmental pollution caused by heavy metals are problems worldwide. In particular, pollution and poisoning by lead ions (Pb2+) continue to be common and serious problems. Hence, there is a need for a widely usable method to easily detect Pb2+ from solutions containing organic materials from environmental water such as seas, ponds, etc. Here, we established a system to easily detect Pb2+ from such mixture solutions using Pb2+ binding peptide immobilized beads (peptidyl beads) and ultraviolet (UV) absorption measurements. This method could detect Pb2+ at low concentrations equivalent to inductively coupled plasmon-atomic emission spectroscopy (ICP-AES). Using the detected values to create a calibration curve, it was found that there was a positive correlation between the concentration of Pb2+ and absorbance, which also made it possible to quantify sub-µM Pb2+ in the solutions. Furthermore, Pb2+ was detected and quantified under mixed conditions of environmental water such as seas, rivers, and ponds. This method is expected to become a versatile and easy-to-use Pb2+ detection method for end-users worldwide.https://doi.org/10.1038/s41598-025-86082-zEnvironmental measurementPeptide immobilized microbeadsLead pollutionUV absorption measurementHeavy metal pollution |
| spellingShingle | Shuhei Yoshida Koki Yoshida Yoshio Hamada Takaaki Tsuruoka Kenji Usui Development of detection system for lead ions in mixture solutions using UV-Vis measurements with peptide immobilized microbeads Scientific Reports Environmental measurement Peptide immobilized microbeads Lead pollution UV absorption measurement Heavy metal pollution |
| title | Development of detection system for lead ions in mixture solutions using UV-Vis measurements with peptide immobilized microbeads |
| title_full | Development of detection system for lead ions in mixture solutions using UV-Vis measurements with peptide immobilized microbeads |
| title_fullStr | Development of detection system for lead ions in mixture solutions using UV-Vis measurements with peptide immobilized microbeads |
| title_full_unstemmed | Development of detection system for lead ions in mixture solutions using UV-Vis measurements with peptide immobilized microbeads |
| title_short | Development of detection system for lead ions in mixture solutions using UV-Vis measurements with peptide immobilized microbeads |
| title_sort | development of detection system for lead ions in mixture solutions using uv vis measurements with peptide immobilized microbeads |
| topic | Environmental measurement Peptide immobilized microbeads Lead pollution UV absorption measurement Heavy metal pollution |
| url | https://doi.org/10.1038/s41598-025-86082-z |
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