Comparison of Different Irrigants in the Removal of Endotoxins and Cultivable Microorganisms from Infected Root Canals
This study was conducted to compare the effectiveness of different irrigants used to remove endotoxins and cultivable microorganisms during endodontic therapy. Forty root canals were contaminated and divided into groups according to the irrigant: 2% NaOCl + surfactant, 2% CHX, 2.5% NaOCl, and pyrog...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2015/125636 |
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| author | Marcia Carneiro Valera Flávia Goulart da Rosa Cardoso Adriana Chung Ana Cláudia Carvalho Xavier Mariana Diehl Figueiredo Frederico Canato Martinho Renato Miotto Palo |
| author_facet | Marcia Carneiro Valera Flávia Goulart da Rosa Cardoso Adriana Chung Ana Cláudia Carvalho Xavier Mariana Diehl Figueiredo Frederico Canato Martinho Renato Miotto Palo |
| author_sort | Marcia Carneiro Valera |
| collection | DOAJ |
| description | This study was conducted to compare the effectiveness of different irrigants used to remove endotoxins and cultivable microorganisms during endodontic therapy. Forty root canals were contaminated and divided into groups according to the irrigant: 2% NaOCl + surfactant, 2% CHX, 2.5% NaOCl, and pyrogen-free saline solution (control). Samples were collected after root canal contamination (S1), after instrumentation (S2), and 7 days after instrumentation (S3). Microorganisms and endotoxins were recovered from 100% of the contaminated root canals (S1). At S2, 2% NaOCl + surfactant, 2% CHX, and 2.5% NaOCl were able to completely eliminate cultivable microorganisms. At S3, both 2% CHX and 2.5% NaOCl were effective in preventing C. albicans and E. coli regrowth, but E. faecalis was still detected. No microorganism species was recovered from root canals instrumented with 2% NaOCl + surfactant. At S2, a higher percentage value of endotoxin reduction was found for 2% NaOCl + surfactant (99.3%) compared to 2% CHX (98.9%) and 2.5% NaOCl (97.18%) (p<0.05). Moreover, at S3, 2% NaOCl + surfactant (100%) was the most effective irrigant against endotoxins. All irrigants tested were effective in reducing microorganisms and endotoxins from root canals. Moreover, 2% NaOCl + surfactant was the most effective irrigant against endotoxins and regrowth of microorganisms. |
| format | Article |
| id | doaj-art-8854e1b2df684d6d9afe4ba6228c0cb0 |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-8854e1b2df684d6d9afe4ba6228c0cb02025-02-03T01:23:51ZengWileyThe Scientific World Journal2356-61401537-744X2015-01-01201510.1155/2015/125636125636Comparison of Different Irrigants in the Removal of Endotoxins and Cultivable Microorganisms from Infected Root CanalsMarcia Carneiro Valera0Flávia Goulart da Rosa Cardoso1Adriana Chung2Ana Cláudia Carvalho Xavier3Mariana Diehl Figueiredo4Frederico Canato Martinho5Renato Miotto Palo6Department of Restorative Dentistry, Institute of Science and Technology, Universidade Estadual Paulista (UNESP), São José dos Campos 12245-000, SP, BrazilDepartment of Restorative Dentistry, Institute of Science and Technology, Universidade Estadual Paulista (UNESP), São José dos Campos 12245-000, SP, BrazilDepartment of Restorative Dentistry, Institute of Science and Technology, Universidade Estadual Paulista (UNESP), São José dos Campos 12245-000, SP, BrazilDepartment of Restorative Dentistry, Institute of Science and Technology, Universidade Estadual Paulista (UNESP), São José dos Campos 12245-000, SP, BrazilDepartment of Restorative Dentistry, Institute of Science and Technology, Universidade Estadual Paulista (UNESP), São José dos Campos 12245-000, SP, BrazilDepartment of Restorative Dentistry, Institute of Science and Technology, Universidade Estadual Paulista (UNESP), São José dos Campos 12245-000, SP, BrazilDepartment of Restorative Dentistry, Institute of Science and Technology, Universidade Estadual Paulista (UNESP), São José dos Campos 12245-000, SP, BrazilThis study was conducted to compare the effectiveness of different irrigants used to remove endotoxins and cultivable microorganisms during endodontic therapy. Forty root canals were contaminated and divided into groups according to the irrigant: 2% NaOCl + surfactant, 2% CHX, 2.5% NaOCl, and pyrogen-free saline solution (control). Samples were collected after root canal contamination (S1), after instrumentation (S2), and 7 days after instrumentation (S3). Microorganisms and endotoxins were recovered from 100% of the contaminated root canals (S1). At S2, 2% NaOCl + surfactant, 2% CHX, and 2.5% NaOCl were able to completely eliminate cultivable microorganisms. At S3, both 2% CHX and 2.5% NaOCl were effective in preventing C. albicans and E. coli regrowth, but E. faecalis was still detected. No microorganism species was recovered from root canals instrumented with 2% NaOCl + surfactant. At S2, a higher percentage value of endotoxin reduction was found for 2% NaOCl + surfactant (99.3%) compared to 2% CHX (98.9%) and 2.5% NaOCl (97.18%) (p<0.05). Moreover, at S3, 2% NaOCl + surfactant (100%) was the most effective irrigant against endotoxins. All irrigants tested were effective in reducing microorganisms and endotoxins from root canals. Moreover, 2% NaOCl + surfactant was the most effective irrigant against endotoxins and regrowth of microorganisms.http://dx.doi.org/10.1155/2015/125636 |
| spellingShingle | Marcia Carneiro Valera Flávia Goulart da Rosa Cardoso Adriana Chung Ana Cláudia Carvalho Xavier Mariana Diehl Figueiredo Frederico Canato Martinho Renato Miotto Palo Comparison of Different Irrigants in the Removal of Endotoxins and Cultivable Microorganisms from Infected Root Canals The Scientific World Journal |
| title | Comparison of Different Irrigants in the Removal of Endotoxins and Cultivable Microorganisms from Infected Root Canals |
| title_full | Comparison of Different Irrigants in the Removal of Endotoxins and Cultivable Microorganisms from Infected Root Canals |
| title_fullStr | Comparison of Different Irrigants in the Removal of Endotoxins and Cultivable Microorganisms from Infected Root Canals |
| title_full_unstemmed | Comparison of Different Irrigants in the Removal of Endotoxins and Cultivable Microorganisms from Infected Root Canals |
| title_short | Comparison of Different Irrigants in the Removal of Endotoxins and Cultivable Microorganisms from Infected Root Canals |
| title_sort | comparison of different irrigants in the removal of endotoxins and cultivable microorganisms from infected root canals |
| url | http://dx.doi.org/10.1155/2015/125636 |
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