A Cocktail Method for Promoting Cardiomyocyte Differentiation from Bone Marrow-Derived Mesenchymal Stem Cells
A growing body of evidence supports the argument that bone marrow-derived mesenchymal stem cells (MSCs) can differentiate into cardiomyocyte-like cells in an appropriate cellular environment, but the differentiation rate is low. A cocktail method was designed: we investigated the role of 5-azacytidi...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2014/162024 |
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| _version_ | 1832553274654326784 |
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| author | Qing Gao Maojuan Guo Xijuan Jiang Xiantong Hu Yijing Wang Yingchang Fan |
| author_facet | Qing Gao Maojuan Guo Xijuan Jiang Xiantong Hu Yijing Wang Yingchang Fan |
| author_sort | Qing Gao |
| collection | DOAJ |
| description | A growing body of evidence supports the argument that bone marrow-derived mesenchymal stem cells (MSCs) can differentiate into cardiomyocyte-like cells in an appropriate cellular environment, but the differentiation rate is low. A cocktail method was designed: we investigated the role of 5-azacytidine (5-aza), salvianolic acid B (SalB), and cardiomyocyte lysis medium (CLM) in inducing MSCs to acquire the phenotypical characteristics of cardiomyocytes. The fourth-passage MSCs were treated with 5-aza, SalB, CLM, 5-aza+salB, 5-aza+CLM, SalB+CLM, and 5-aza+SalB+CLM for 2 weeks. Immunofluorescence results showed that cTnT expression in the 5-aza+salB+CLM group was stronger than other groups. Real-time qPCR and Western blotting analyses showed that cTnT, alpha-cardiac actin, mef-2c, Cx43, and GSK-3beta expression increased while beta-catenin expression decreased. The salB+5-aza+CLM group had the most evident effects. SalB combined with 5-aza and CLM improved cardiomyocyte differentiation from MSCs. In the MSCs differentiation process, the Wnt/beta-catenin signaling pathway had been inhibited. |
| format | Article |
| id | doaj-art-8850d7142a61436dbefb0ad42a6676d4 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-8850d7142a61436dbefb0ad42a6676d42025-02-03T05:54:22ZengWileyStem Cells International1687-966X1687-96782014-01-01201410.1155/2014/162024162024A Cocktail Method for Promoting Cardiomyocyte Differentiation from Bone Marrow-Derived Mesenchymal Stem CellsQing Gao0Maojuan Guo1Xijuan Jiang2Xiantong Hu3Yijing Wang4Yingchang Fan5Key Laboratory of Pathology of State Administration of Traditional Chinese Medicine, School of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, ChinaKey Laboratory of Pathology of State Administration of Traditional Chinese Medicine, School of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, ChinaKey Laboratory of Pathology of State Administration of Traditional Chinese Medicine, School of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, ChinaKey Laboratory of Pathology of State Administration of Traditional Chinese Medicine, School of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, ChinaKey Laboratory of Pathology of State Administration of Traditional Chinese Medicine, School of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, ChinaKey Laboratory of Pathology of State Administration of Traditional Chinese Medicine, School of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, ChinaA growing body of evidence supports the argument that bone marrow-derived mesenchymal stem cells (MSCs) can differentiate into cardiomyocyte-like cells in an appropriate cellular environment, but the differentiation rate is low. A cocktail method was designed: we investigated the role of 5-azacytidine (5-aza), salvianolic acid B (SalB), and cardiomyocyte lysis medium (CLM) in inducing MSCs to acquire the phenotypical characteristics of cardiomyocytes. The fourth-passage MSCs were treated with 5-aza, SalB, CLM, 5-aza+salB, 5-aza+CLM, SalB+CLM, and 5-aza+SalB+CLM for 2 weeks. Immunofluorescence results showed that cTnT expression in the 5-aza+salB+CLM group was stronger than other groups. Real-time qPCR and Western blotting analyses showed that cTnT, alpha-cardiac actin, mef-2c, Cx43, and GSK-3beta expression increased while beta-catenin expression decreased. The salB+5-aza+CLM group had the most evident effects. SalB combined with 5-aza and CLM improved cardiomyocyte differentiation from MSCs. In the MSCs differentiation process, the Wnt/beta-catenin signaling pathway had been inhibited.http://dx.doi.org/10.1155/2014/162024 |
| spellingShingle | Qing Gao Maojuan Guo Xijuan Jiang Xiantong Hu Yijing Wang Yingchang Fan A Cocktail Method for Promoting Cardiomyocyte Differentiation from Bone Marrow-Derived Mesenchymal Stem Cells Stem Cells International |
| title | A Cocktail Method for Promoting Cardiomyocyte Differentiation from Bone Marrow-Derived Mesenchymal Stem Cells |
| title_full | A Cocktail Method for Promoting Cardiomyocyte Differentiation from Bone Marrow-Derived Mesenchymal Stem Cells |
| title_fullStr | A Cocktail Method for Promoting Cardiomyocyte Differentiation from Bone Marrow-Derived Mesenchymal Stem Cells |
| title_full_unstemmed | A Cocktail Method for Promoting Cardiomyocyte Differentiation from Bone Marrow-Derived Mesenchymal Stem Cells |
| title_short | A Cocktail Method for Promoting Cardiomyocyte Differentiation from Bone Marrow-Derived Mesenchymal Stem Cells |
| title_sort | cocktail method for promoting cardiomyocyte differentiation from bone marrow derived mesenchymal stem cells |
| url | http://dx.doi.org/10.1155/2014/162024 |
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