Aloperin improves liver non-alcoholic steatohepatitis in vitro and in vivo
Introduction The aim of this work is to investigate the inhibitory effect of aloperin (Alo) on hepatocyte apoptosis in non-alcoholic fatty liver disease, and the underlying mechanism. Material and methods Rats in the Alo groups were fed a high-fat + high-sugar diet for 8 weeks and then treated with...
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| Format: | Article |
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Termedia Publishing House
2020-05-01
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| Series: | Archives of Medical Science |
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| Online Access: | https://www.archivesofmedicalscience.com/Aloperin-improves-liver-non-alcoholic-steatohepatitis-n-in-vitro-and-in-vivo-,118004,0,2.html |
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| author | Zhongsheng Guo Ruyu Liu Liwei Guo Sumei Lu Min Yang Shaofei Wu Yong An |
| author_facet | Zhongsheng Guo Ruyu Liu Liwei Guo Sumei Lu Min Yang Shaofei Wu Yong An |
| author_sort | Zhongsheng Guo |
| collection | DOAJ |
| description | Introduction
The aim of this work is to investigate the inhibitory effect of aloperin (Alo) on hepatocyte apoptosis in non-alcoholic fatty liver disease, and the underlying mechanism.
Material and methods
Rats in the Alo groups were fed a high-fat + high-sugar diet for 8 weeks and then treated with low-, moderate-, and high-dose Alo for another 8 weeks via gavage. Oxidative stress indices were tested by a colourimetric method, and pathological changes were observed by haematoxylin–eosin staining. Apoptosis was detected by TUNEL staining. TLR4, TRIF, and NF-κB(p65) mRNA and protein expressions were detected by RT-qPCR, Western blot assay and immunohistochemistry. In the in vitro study, L02 cells were treated with FFA (free fatty acid) for 24 h to establish a non-alcoholic steatohepatitis (NASH) model. Inhibition of cell proliferation was measured by the MTT method, and cell apoptosis was evaluated by flow cytometry. Finally, the nuclear import volume of NF-κB(p65) was evaluated by cellular immunofluorescence.
Results
Cell apoptosis significantly decreased in the Alo-treatment groups in a dose-dependent manner (p < 0.05). TLR4, TRIF, and NF-κB(p65) expression in the Alo-treatment groups was significantly downregulated compared with model group (p < 0.05). The cell proliferation rate significantly increased, cell apoptosis significantly decreased (p < 0.05), and the TLR4/TRIF/NF-κB pathway was significantly inhibited (p < 0.05) in the Alo-treatment groups. The nuclear import volume of NF-κB(p65) in the Alo-treatment groups was significantly decreased compared with that in the model group in a dose-dependent manger (p < 0.05).
Conclusions
Alo could improve NASH via the TLR4/TRIF/NF-κB pathway. |
| format | Article |
| id | doaj-art-884a93e5f144425cb0de235e98372c7f |
| institution | OA Journals |
| issn | 1734-1922 1896-9151 |
| language | English |
| publishDate | 2020-05-01 |
| publisher | Termedia Publishing House |
| record_format | Article |
| series | Archives of Medical Science |
| spelling | doaj-art-884a93e5f144425cb0de235e98372c7f2025-08-20T02:31:34ZengTermedia Publishing HouseArchives of Medical Science1734-19221896-91512020-05-0121128529710.5114/aoms.2020.95629118004Aloperin improves liver non-alcoholic steatohepatitis in vitro and in vivoZhongsheng Guo0Ruyu Liu1Liwei Guo2Sumei Lu3Min Yang4Shaofei Wu5Yong An6Department of Infection Diseases and Liver Diseases, The First Affiliated Hospital of Shandong First Medical University (Shandong Provincial Qianfoshan Hospital), Jinan, ChinaDepartment of Hepatology, Beijing Ditan Hospital Capital Medical University, Beijing, ChinaDepartment of Infection Diseases and Liver Diseases, The First Affiliated Hospital of Shandong First Medical University (Shandong Provincial Qianfoshan Hospital), Jinan, ChinaPCR laboratory,The First Affiliated Hospital of Shandong First Medical University (Shandong Provincial Qianfoshan Hospital), Jinan, ChinaDepartment of Clinical Pathology, The First Affiliated Hospital of Shandong First Medical University (Shandong Provincial Qianfoshan Hospital), Jinan, ChinaDepartment of Infection Diseases and Liver Diseases, The First Affiliated Hospital of Shandong First Medical University (Shandong Provincial Qianfoshan Hospital), Jinan, ChinaDepartment of Infection Diseases and Liver Diseases, The First Affiliated Hospital of Shandong First Medical University (Shandong Provincial Qianfoshan Hospital), Jinan, ChinaIntroduction The aim of this work is to investigate the inhibitory effect of aloperin (Alo) on hepatocyte apoptosis in non-alcoholic fatty liver disease, and the underlying mechanism. Material and methods Rats in the Alo groups were fed a high-fat + high-sugar diet for 8 weeks and then treated with low-, moderate-, and high-dose Alo for another 8 weeks via gavage. Oxidative stress indices were tested by a colourimetric method, and pathological changes were observed by haematoxylin–eosin staining. Apoptosis was detected by TUNEL staining. TLR4, TRIF, and NF-κB(p65) mRNA and protein expressions were detected by RT-qPCR, Western blot assay and immunohistochemistry. In the in vitro study, L02 cells were treated with FFA (free fatty acid) for 24 h to establish a non-alcoholic steatohepatitis (NASH) model. Inhibition of cell proliferation was measured by the MTT method, and cell apoptosis was evaluated by flow cytometry. Finally, the nuclear import volume of NF-κB(p65) was evaluated by cellular immunofluorescence. Results Cell apoptosis significantly decreased in the Alo-treatment groups in a dose-dependent manner (p < 0.05). TLR4, TRIF, and NF-κB(p65) expression in the Alo-treatment groups was significantly downregulated compared with model group (p < 0.05). The cell proliferation rate significantly increased, cell apoptosis significantly decreased (p < 0.05), and the TLR4/TRIF/NF-κB pathway was significantly inhibited (p < 0.05) in the Alo-treatment groups. The nuclear import volume of NF-κB(p65) in the Alo-treatment groups was significantly decreased compared with that in the model group in a dose-dependent manger (p < 0.05). Conclusions Alo could improve NASH via the TLR4/TRIF/NF-κB pathway.https://www.archivesofmedicalscience.com/Aloperin-improves-liver-non-alcoholic-steatohepatitis-n-in-vitro-and-in-vivo-,118004,0,2.htmlaloperinnon-alcoholic steatohepatitistlr4/trif/nf-κbcell apoptosis |
| spellingShingle | Zhongsheng Guo Ruyu Liu Liwei Guo Sumei Lu Min Yang Shaofei Wu Yong An Aloperin improves liver non-alcoholic steatohepatitis in vitro and in vivo Archives of Medical Science aloperin non-alcoholic steatohepatitis tlr4/trif/nf-κb cell apoptosis |
| title | Aloperin improves liver non-alcoholic steatohepatitis
in vitro and in vivo |
| title_full | Aloperin improves liver non-alcoholic steatohepatitis
in vitro and in vivo |
| title_fullStr | Aloperin improves liver non-alcoholic steatohepatitis
in vitro and in vivo |
| title_full_unstemmed | Aloperin improves liver non-alcoholic steatohepatitis
in vitro and in vivo |
| title_short | Aloperin improves liver non-alcoholic steatohepatitis
in vitro and in vivo |
| title_sort | aloperin improves liver non alcoholic steatohepatitis in vitro and in vivo |
| topic | aloperin non-alcoholic steatohepatitis tlr4/trif/nf-κb cell apoptosis |
| url | https://www.archivesofmedicalscience.com/Aloperin-improves-liver-non-alcoholic-steatohepatitis-n-in-vitro-and-in-vivo-,118004,0,2.html |
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