Subetta Treatment Increases Adiponectin Secretion by Mature Human Adipocytes In Vitro
Purpose. To investigate the mechanism of action in peripheral tissues of novel complex drug containing release-active dilutions of antibodies to the beta subunit of the insulin receptor and antibodies to endothelial nitric oxide synthase (Subetta), which has shown efficacy in animal models of diabet...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | International Journal of Endocrinology |
| Online Access: | http://dx.doi.org/10.1155/2013/925874 |
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| _version_ | 1832565657819938816 |
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| author | Jim Nicoll Evgeniy A. Gorbunov Sergey A. Tarasov Oleg I. Epstein |
| author_facet | Jim Nicoll Evgeniy A. Gorbunov Sergey A. Tarasov Oleg I. Epstein |
| author_sort | Jim Nicoll |
| collection | DOAJ |
| description | Purpose. To investigate the mechanism of action in peripheral tissues of novel complex drug containing release-active dilutions of antibodies to the beta subunit of the insulin receptor and antibodies to endothelial nitric oxide synthase (Subetta), which has shown efficacy in animal models of diabetes. Methods. Human mature adipocytes were incubated either with Subetta, with one of negative controls (placebo or vehicle), with one of nonspecific controls (release-active dilutions of antibodies to cannabinoid receptor type I or release-active dilutions of rabbit nonimmune serum), or with dimethyl sulfoxide (DMSO) at 37°C in a humidified incubator at 5% CO2 for three days. Rosiglitazone was used as reference drug. Secretion of adiponectin was measured by quantitative enzyme-linked immunosorbent assay (ELISA). Results. Only Subetta significantly stimulates adiponectin production by mature human adipocytes. Nonspecific controls did not significantly affect adiponectin secretion, resulting in adiponectin levels comparable to background values of the negative controls and DMSO. Conclusion. Increasing adiponectin production in absence of insulin by Subetta probably via modulating effect on the beta subunit of the insulin receptor might serve as one of the mechanisms of the antidiabetic effect of this drug. These in vitro results give first insight on possible mechanism of action of Subetta and serve as a background for further studies. |
| format | Article |
| id | doaj-art-883a154d24fe4d4b97baeada8fe84936 |
| institution | Kabale University |
| issn | 1687-8337 1687-8345 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Endocrinology |
| spelling | doaj-art-883a154d24fe4d4b97baeada8fe849362025-02-03T01:07:03ZengWileyInternational Journal of Endocrinology1687-83371687-83452013-01-01201310.1155/2013/925874925874Subetta Treatment Increases Adiponectin Secretion by Mature Human Adipocytes In VitroJim Nicoll0Evgeniy A. Gorbunov1Sergey A. Tarasov2Oleg I. Epstein3Zen-Bio, Inc., 3200 Chapel Hill-Nelson Boulevard, Street 100, Research Triangle Park, NC 27709, USAOOO “NPF “MATERIA MEDICA HOLDING”, 3rd Samotyochny per., 9, Moscow 127473, RussiaOOO “NPF “MATERIA MEDICA HOLDING”, 3rd Samotyochny per., 9, Moscow 127473, RussiaOOO “NPF “MATERIA MEDICA HOLDING”, 3rd Samotyochny per., 9, Moscow 127473, RussiaPurpose. To investigate the mechanism of action in peripheral tissues of novel complex drug containing release-active dilutions of antibodies to the beta subunit of the insulin receptor and antibodies to endothelial nitric oxide synthase (Subetta), which has shown efficacy in animal models of diabetes. Methods. Human mature adipocytes were incubated either with Subetta, with one of negative controls (placebo or vehicle), with one of nonspecific controls (release-active dilutions of antibodies to cannabinoid receptor type I or release-active dilutions of rabbit nonimmune serum), or with dimethyl sulfoxide (DMSO) at 37°C in a humidified incubator at 5% CO2 for three days. Rosiglitazone was used as reference drug. Secretion of adiponectin was measured by quantitative enzyme-linked immunosorbent assay (ELISA). Results. Only Subetta significantly stimulates adiponectin production by mature human adipocytes. Nonspecific controls did not significantly affect adiponectin secretion, resulting in adiponectin levels comparable to background values of the negative controls and DMSO. Conclusion. Increasing adiponectin production in absence of insulin by Subetta probably via modulating effect on the beta subunit of the insulin receptor might serve as one of the mechanisms of the antidiabetic effect of this drug. These in vitro results give first insight on possible mechanism of action of Subetta and serve as a background for further studies.http://dx.doi.org/10.1155/2013/925874 |
| spellingShingle | Jim Nicoll Evgeniy A. Gorbunov Sergey A. Tarasov Oleg I. Epstein Subetta Treatment Increases Adiponectin Secretion by Mature Human Adipocytes In Vitro International Journal of Endocrinology |
| title | Subetta Treatment Increases Adiponectin Secretion by Mature Human Adipocytes In Vitro |
| title_full | Subetta Treatment Increases Adiponectin Secretion by Mature Human Adipocytes In Vitro |
| title_fullStr | Subetta Treatment Increases Adiponectin Secretion by Mature Human Adipocytes In Vitro |
| title_full_unstemmed | Subetta Treatment Increases Adiponectin Secretion by Mature Human Adipocytes In Vitro |
| title_short | Subetta Treatment Increases Adiponectin Secretion by Mature Human Adipocytes In Vitro |
| title_sort | subetta treatment increases adiponectin secretion by mature human adipocytes in vitro |
| url | http://dx.doi.org/10.1155/2013/925874 |
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