Proteomic Analysis of Saliva Identifies Potential Biomarkers for Orthodontic Tooth Movement
Orthodontic treatment has been shown to induce inflammation, followed by bone remodelling in the periodontium. These processes trigger the secretion of various proteins and enzymes into the saliva. This study aims to identify salivary proteins that change in expression during orthodontic tooth movem...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/2012/647240 |
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| _version_ | 1832560955965308928 |
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| author | Mohd Faiz Ellias Shahrul Hisham Zainal Ariffin Saiful Anuar Karsani Mariati Abdul Rahman Shahidan Senafi Rohaya Megat Abdul Wahab |
| author_facet | Mohd Faiz Ellias Shahrul Hisham Zainal Ariffin Saiful Anuar Karsani Mariati Abdul Rahman Shahidan Senafi Rohaya Megat Abdul Wahab |
| author_sort | Mohd Faiz Ellias |
| collection | DOAJ |
| description | Orthodontic treatment has been shown to induce inflammation, followed by bone remodelling in the periodontium. These processes trigger the secretion of various proteins and enzymes into the saliva. This study aims to identify salivary proteins that change in expression during orthodontic tooth movement. These differentially expressed proteins can potentially serve as protein biomarkers for the monitoring of orthodontic treatment and tooth movement. Whole saliva from three healthy female subjects were collected before force application using fixed appliance and at 14 days after 0.014′′ Niti wire was applied. Salivary proteins were resolved using two-dimensional gel electrophoresis (2DE) over a pH range of 3–10, and the resulting proteome profiles were compared. Differentially expressed protein spots were then identified by MALDI-TOF/TOF tandem mass spectrometry. Nine proteins were found to be differentially expressed; however, only eight were identified by MALDI-TOF/TOF. Four of these proteins—Protein S100-A9, immunoglobulin J chain, Ig alpha-1 chain C region, and CRISP-3—have known roles in inflammation and bone resorption. |
| format | Article |
| id | doaj-art-881bf4e2defd4f0a83b6d18edffbe347 |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-881bf4e2defd4f0a83b6d18edffbe3472025-02-03T01:26:15ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/647240647240Proteomic Analysis of Saliva Identifies Potential Biomarkers for Orthodontic Tooth MovementMohd Faiz Ellias0Shahrul Hisham Zainal Ariffin1Saiful Anuar Karsani2Mariati Abdul Rahman3Shahidan Senafi4Rohaya Megat Abdul Wahab5School of Bioscience and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Selangor, 43600 Bangi, MalaysiaSchool of Bioscience and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Selangor, 43600 Bangi, MalaysiaInstitute of Biological Sciences, Faculty of Science, University of Malaya and University of Malaya Centre for Proteomics Research (UMCPR), Universiti Kebangsaan Malaysia, 50603 Kuala Lumpur, MalaysiaClinical Oral Biology Department, Faculty of Dentistry, Universiti Kebangsaan Malaysia, 50300 Kuala Lumpur, MalaysiaSchool of Bioscience and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Selangor, 43600 Bangi, MalaysiaOrthodontic Department, Faculty of Dentistry, Universiti Kebangsaan Malaysia, 50300 Kuala Lumpur, MalaysiaOrthodontic treatment has been shown to induce inflammation, followed by bone remodelling in the periodontium. These processes trigger the secretion of various proteins and enzymes into the saliva. This study aims to identify salivary proteins that change in expression during orthodontic tooth movement. These differentially expressed proteins can potentially serve as protein biomarkers for the monitoring of orthodontic treatment and tooth movement. Whole saliva from three healthy female subjects were collected before force application using fixed appliance and at 14 days after 0.014′′ Niti wire was applied. Salivary proteins were resolved using two-dimensional gel electrophoresis (2DE) over a pH range of 3–10, and the resulting proteome profiles were compared. Differentially expressed protein spots were then identified by MALDI-TOF/TOF tandem mass spectrometry. Nine proteins were found to be differentially expressed; however, only eight were identified by MALDI-TOF/TOF. Four of these proteins—Protein S100-A9, immunoglobulin J chain, Ig alpha-1 chain C region, and CRISP-3—have known roles in inflammation and bone resorption.http://dx.doi.org/10.1100/2012/647240 |
| spellingShingle | Mohd Faiz Ellias Shahrul Hisham Zainal Ariffin Saiful Anuar Karsani Mariati Abdul Rahman Shahidan Senafi Rohaya Megat Abdul Wahab Proteomic Analysis of Saliva Identifies Potential Biomarkers for Orthodontic Tooth Movement The Scientific World Journal |
| title | Proteomic Analysis of Saliva Identifies Potential Biomarkers for Orthodontic Tooth Movement |
| title_full | Proteomic Analysis of Saliva Identifies Potential Biomarkers for Orthodontic Tooth Movement |
| title_fullStr | Proteomic Analysis of Saliva Identifies Potential Biomarkers for Orthodontic Tooth Movement |
| title_full_unstemmed | Proteomic Analysis of Saliva Identifies Potential Biomarkers for Orthodontic Tooth Movement |
| title_short | Proteomic Analysis of Saliva Identifies Potential Biomarkers for Orthodontic Tooth Movement |
| title_sort | proteomic analysis of saliva identifies potential biomarkers for orthodontic tooth movement |
| url | http://dx.doi.org/10.1100/2012/647240 |
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