The Tomato lncRNA47258-miR319b-TCP Module in Biocontrol Bacteria Sneb821 Induced Plants Resistance to <i>Meloidogyne incognita</i>
Long non-coding RNAs (lncRNAs) represent a class of non-coding RNAs. In the study of <i>Pseudomonas putida</i> Sneb821-induced tomato resistance to <i>Meloidogyne incognita</i>, reverse transcription polymerase chain reaction (RT-PCR) was employed to validate 12 lncRNAs in to...
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MDPI AG
2025-03-01
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| author | Fan Yang Xiaoxiao Wu Lijie Chen Mingfang Qi |
| author_facet | Fan Yang Xiaoxiao Wu Lijie Chen Mingfang Qi |
| author_sort | Fan Yang |
| collection | DOAJ |
| description | Long non-coding RNAs (lncRNAs) represent a class of non-coding RNAs. In the study of <i>Pseudomonas putida</i> Sneb821-induced tomato resistance to <i>Meloidogyne incognita</i>, reverse transcription polymerase chain reaction (RT-PCR) was employed to validate 12 lncRNAs in tomato. Among them, the lncRNA47258/miR319b/TCP molecular regulatory module was likely implicated in the process of Sneb821-induced tomato resistance against <i>M. incognita</i>. Through the application of tomato hairy root and virus-induced gene silencing (VIGS) technologies for the investigation of lncRNA47258, it was determined that lncRNA47258 could target the <i>TCP</i> (<i>Solyc07g062681.1</i>) gene and modulate the metabolic pathway of tomato jasmonic acid-related indices, thereby impeding the infection of <i>M. incognita</i>. Moreover, the overexpression of the target gene <i>TCP</i> (<i>Solyc07g062681.1</i>) using tomato hairy root technology demonstrated that it could regulate the jasmonic acid synthesis pathway in tomato, consequently obstructing the infection and suppressing the development of <i>M. incognita</i>. Collectively, lncRNA47258/miR319b/<i>TCP</i> (<i>Solyc07g062681.1</i>) was preliminarily verified to be involved in the Sneb821-induced resistance process against <i>M. incognita</i> in tomato. |
| format | Article |
| id | doaj-art-881aa9ed0b944b5db46f4e783882e9c4 |
| institution | DOAJ |
| issn | 2076-0817 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
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| series | Pathogens |
| spelling | doaj-art-881aa9ed0b944b5db46f4e783882e9c42025-08-20T02:42:28ZengMDPI AGPathogens2076-08172025-03-0114325610.3390/pathogens14030256The Tomato lncRNA47258-miR319b-TCP Module in Biocontrol Bacteria Sneb821 Induced Plants Resistance to <i>Meloidogyne incognita</i>Fan Yang0Xiaoxiao Wu1Lijie Chen2Mingfang Qi3College of Horticulture, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Plant Protection, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Plant Protection, Shenyang Agricultural University, Shenyang 110866, ChinaCollege of Horticulture, Shenyang Agricultural University, Shenyang 110866, ChinaLong non-coding RNAs (lncRNAs) represent a class of non-coding RNAs. In the study of <i>Pseudomonas putida</i> Sneb821-induced tomato resistance to <i>Meloidogyne incognita</i>, reverse transcription polymerase chain reaction (RT-PCR) was employed to validate 12 lncRNAs in tomato. Among them, the lncRNA47258/miR319b/TCP molecular regulatory module was likely implicated in the process of Sneb821-induced tomato resistance against <i>M. incognita</i>. Through the application of tomato hairy root and virus-induced gene silencing (VIGS) technologies for the investigation of lncRNA47258, it was determined that lncRNA47258 could target the <i>TCP</i> (<i>Solyc07g062681.1</i>) gene and modulate the metabolic pathway of tomato jasmonic acid-related indices, thereby impeding the infection of <i>M. incognita</i>. Moreover, the overexpression of the target gene <i>TCP</i> (<i>Solyc07g062681.1</i>) using tomato hairy root technology demonstrated that it could regulate the jasmonic acid synthesis pathway in tomato, consequently obstructing the infection and suppressing the development of <i>M. incognita</i>. Collectively, lncRNA47258/miR319b/<i>TCP</i> (<i>Solyc07g062681.1</i>) was preliminarily verified to be involved in the Sneb821-induced resistance process against <i>M. incognita</i> in tomato.https://www.mdpi.com/2076-0817/14/3/256lncRNAnematodetomatobiocontrol bacteria |
| spellingShingle | Fan Yang Xiaoxiao Wu Lijie Chen Mingfang Qi The Tomato lncRNA47258-miR319b-TCP Module in Biocontrol Bacteria Sneb821 Induced Plants Resistance to <i>Meloidogyne incognita</i> Pathogens lncRNA nematode tomato biocontrol bacteria |
| title | The Tomato lncRNA47258-miR319b-TCP Module in Biocontrol Bacteria Sneb821 Induced Plants Resistance to <i>Meloidogyne incognita</i> |
| title_full | The Tomato lncRNA47258-miR319b-TCP Module in Biocontrol Bacteria Sneb821 Induced Plants Resistance to <i>Meloidogyne incognita</i> |
| title_fullStr | The Tomato lncRNA47258-miR319b-TCP Module in Biocontrol Bacteria Sneb821 Induced Plants Resistance to <i>Meloidogyne incognita</i> |
| title_full_unstemmed | The Tomato lncRNA47258-miR319b-TCP Module in Biocontrol Bacteria Sneb821 Induced Plants Resistance to <i>Meloidogyne incognita</i> |
| title_short | The Tomato lncRNA47258-miR319b-TCP Module in Biocontrol Bacteria Sneb821 Induced Plants Resistance to <i>Meloidogyne incognita</i> |
| title_sort | tomato lncrna47258 mir319b tcp module in biocontrol bacteria sneb821 induced plants resistance to i meloidogyne incognita i |
| topic | lncRNA nematode tomato biocontrol bacteria |
| url | https://www.mdpi.com/2076-0817/14/3/256 |
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