Conditioned Media from Adipose-Tissue-Derived Mesenchymal Stem Cells Downregulate Degradative Mediators Induced by Interleukin-1β in Osteoarthritic Chondrocytes
Osteoarthritis (OA) is the most frequent joint disorder and an important cause of disability. Recent studies have shown the potential of adipose-tissue-derived mesenchymal stem cells (AD-MSC) for cartilage repair. We have investigated whether conditioned medium from AD-MSC (CM) may regulate in OA ch...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2013-01-01
|
| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2013/357014 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832567596622282752 |
|---|---|
| author | Julia Platas Maria Isabel Guillén María Dolores Pérez del Caz Francisco Gomar Vicente Mirabet Maria José Alcaraz |
| author_facet | Julia Platas Maria Isabel Guillén María Dolores Pérez del Caz Francisco Gomar Vicente Mirabet Maria José Alcaraz |
| author_sort | Julia Platas |
| collection | DOAJ |
| description | Osteoarthritis (OA) is the most frequent joint disorder and an important cause of disability. Recent studies have shown the potential of adipose-tissue-derived mesenchymal stem cells (AD-MSC) for cartilage repair. We have investigated whether conditioned medium from AD-MSC (CM) may regulate in OA chondrocytes a number of key mediators involved in cartilage degeneration. CM enhanced type II collagen expression in OA chondrocytes while decreasing matrix metalloproteinase (MMP) activity in cell supernatants as well as the levels of MMP-3 and MMP-13 proteins and mRNA in OA chondrocytes stimulated with interleukin- (IL-) 1β. In addition, CM increased IL-10 levels and counteracted the stimulating effects of IL-1β on the production of tumor necrosis factor-α, IL-6, prostaglandin E2, and NO measured as nitrite and the mRNA expression of these cytokines, CCL-2, CCL-3, CCL-4, CCL-5, CCL-8, CCL-19, CCL-20, CXCL-1, CXCL-2, CXCL-3, CXCL-5, CXCL-8, cyclooxygenase-2, microsomal prostaglandin E synthase-1, and inducible NO synthase. These effects may be dependent on the inhibition of nuclear factor-κB activation by CM. Our data demonstrate the chondroprotective actions of CM and provide support for further studies of this approach in joint disease. |
| format | Article |
| id | doaj-art-880eb8cdac5748f39240d4e8a24e8130 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-880eb8cdac5748f39240d4e8a24e81302025-02-03T01:01:02ZengWileyMediators of Inflammation0962-93511466-18612013-01-01201310.1155/2013/357014357014Conditioned Media from Adipose-Tissue-Derived Mesenchymal Stem Cells Downregulate Degradative Mediators Induced by Interleukin-1β in Osteoarthritic ChondrocytesJulia Platas0Maria Isabel Guillén1María Dolores Pérez del Caz2Francisco Gomar3Vicente Mirabet4Maria José Alcaraz5Department of Pharmacology, University of Valencia, Avenue Vicent Andrés Estellés s/n, Burjassot, 46100 Valencia, SpainDepartment of Pharmacology, University of Valencia, Avenue Vicent Andrés Estellés s/n, Burjassot, 46100 Valencia, SpainDepartment of Burn and Plastic Surgery, La Fe University Hospital, 46026 Valencia, SpainDepartment of Surgery, Faculty of Medicine, University of Valencia, 46010 Valencia, SpainValencia Transfusion Center, Generalitat Valenciana, 46014 Valencia, SpainDepartment of Pharmacology, University of Valencia, Avenue Vicent Andrés Estellés s/n, Burjassot, 46100 Valencia, SpainOsteoarthritis (OA) is the most frequent joint disorder and an important cause of disability. Recent studies have shown the potential of adipose-tissue-derived mesenchymal stem cells (AD-MSC) for cartilage repair. We have investigated whether conditioned medium from AD-MSC (CM) may regulate in OA chondrocytes a number of key mediators involved in cartilage degeneration. CM enhanced type II collagen expression in OA chondrocytes while decreasing matrix metalloproteinase (MMP) activity in cell supernatants as well as the levels of MMP-3 and MMP-13 proteins and mRNA in OA chondrocytes stimulated with interleukin- (IL-) 1β. In addition, CM increased IL-10 levels and counteracted the stimulating effects of IL-1β on the production of tumor necrosis factor-α, IL-6, prostaglandin E2, and NO measured as nitrite and the mRNA expression of these cytokines, CCL-2, CCL-3, CCL-4, CCL-5, CCL-8, CCL-19, CCL-20, CXCL-1, CXCL-2, CXCL-3, CXCL-5, CXCL-8, cyclooxygenase-2, microsomal prostaglandin E synthase-1, and inducible NO synthase. These effects may be dependent on the inhibition of nuclear factor-κB activation by CM. Our data demonstrate the chondroprotective actions of CM and provide support for further studies of this approach in joint disease.http://dx.doi.org/10.1155/2013/357014 |
| spellingShingle | Julia Platas Maria Isabel Guillén María Dolores Pérez del Caz Francisco Gomar Vicente Mirabet Maria José Alcaraz Conditioned Media from Adipose-Tissue-Derived Mesenchymal Stem Cells Downregulate Degradative Mediators Induced by Interleukin-1β in Osteoarthritic Chondrocytes Mediators of Inflammation |
| title | Conditioned Media from Adipose-Tissue-Derived Mesenchymal Stem Cells Downregulate Degradative Mediators Induced by Interleukin-1β in Osteoarthritic Chondrocytes |
| title_full | Conditioned Media from Adipose-Tissue-Derived Mesenchymal Stem Cells Downregulate Degradative Mediators Induced by Interleukin-1β in Osteoarthritic Chondrocytes |
| title_fullStr | Conditioned Media from Adipose-Tissue-Derived Mesenchymal Stem Cells Downregulate Degradative Mediators Induced by Interleukin-1β in Osteoarthritic Chondrocytes |
| title_full_unstemmed | Conditioned Media from Adipose-Tissue-Derived Mesenchymal Stem Cells Downregulate Degradative Mediators Induced by Interleukin-1β in Osteoarthritic Chondrocytes |
| title_short | Conditioned Media from Adipose-Tissue-Derived Mesenchymal Stem Cells Downregulate Degradative Mediators Induced by Interleukin-1β in Osteoarthritic Chondrocytes |
| title_sort | conditioned media from adipose tissue derived mesenchymal stem cells downregulate degradative mediators induced by interleukin 1β in osteoarthritic chondrocytes |
| url | http://dx.doi.org/10.1155/2013/357014 |
| work_keys_str_mv | AT juliaplatas conditionedmediafromadiposetissuederivedmesenchymalstemcellsdownregulatedegradativemediatorsinducedbyinterleukin1binosteoarthriticchondrocytes AT mariaisabelguillen conditionedmediafromadiposetissuederivedmesenchymalstemcellsdownregulatedegradativemediatorsinducedbyinterleukin1binosteoarthriticchondrocytes AT mariadoloresperezdelcaz conditionedmediafromadiposetissuederivedmesenchymalstemcellsdownregulatedegradativemediatorsinducedbyinterleukin1binosteoarthriticchondrocytes AT franciscogomar conditionedmediafromadiposetissuederivedmesenchymalstemcellsdownregulatedegradativemediatorsinducedbyinterleukin1binosteoarthriticchondrocytes AT vicentemirabet conditionedmediafromadiposetissuederivedmesenchymalstemcellsdownregulatedegradativemediatorsinducedbyinterleukin1binosteoarthriticchondrocytes AT mariajosealcaraz conditionedmediafromadiposetissuederivedmesenchymalstemcellsdownregulatedegradativemediatorsinducedbyinterleukin1binosteoarthriticchondrocytes |