Anodized Ti6Al4V-ELI, electroplated with copper is bactericidal against Staphylococcus aureus and enhances macrophage phagocytosis
Abstract Implants aim to restore skeletal dysfunction associated with ageing and trauma, yet infection and ineffective immune responses can lead to failure. This project characterized the microbiological and host cell responses to titanium alloy with or without electroplated metallic copper. Bacteri...
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| Format: | Article |
| Language: | English |
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Springer
2025-01-01
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| Series: | Journal of Materials Science: Materials in Medicine |
| Online Access: | https://doi.org/10.1007/s10856-024-06853-4 |
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| author | Paula Milena Giraldo-Osorno Adam Benedict Turner Sebastião Mollet Barros Robin Büscher Simone Guttau Farah Asa’ad Margarita Trobos Anders Palmquist |
| author_facet | Paula Milena Giraldo-Osorno Adam Benedict Turner Sebastião Mollet Barros Robin Büscher Simone Guttau Farah Asa’ad Margarita Trobos Anders Palmquist |
| author_sort | Paula Milena Giraldo-Osorno |
| collection | DOAJ |
| description | Abstract Implants aim to restore skeletal dysfunction associated with ageing and trauma, yet infection and ineffective immune responses can lead to failure. This project characterized the microbiological and host cell responses to titanium alloy with or without electroplated metallic copper. Bacterial viability counting and scanning electron microscopy quantified and visualized the direct and indirect bactericidal effects of the Cu-electroplated titanium (Cu-Ep-Ti) against two different Staphylococcus aureus strains. Human THP-1 macrophage adhesion and viability was analyzed, along with phagocytosis. Results showed potent antimicrobial activity alongside promising host-immunomodulatory properties. Direct and indirect exposure to Cu-Ep-Ti produced potent bactericidal effects resulting in 94–100% reductions in bacterial viability at 24 h, with complete eradication in some cases. As expected, cytotoxicity was observed in THP-1 macrophages without media exchange, though when media was exchanged at 8, 24 and 48 h cell viability was equivalent to Control-Ti. Interestingly macrophages adhered to the copper material or grown in the presence of copper ions showed 7-fold increase in phagocytosis of S. aureus bioparticles compared to Control-Ti, suggesting a dual bactericidal and host immunomodulatory mechanism. In conclusion, this Cu-electroplated Ti biomaterial can limit bacterial contamination on the implant surface, whilst simultaneously promoting a beneficial antimicrobial immune response. Graphical Abstract |
| format | Article |
| id | doaj-art-87f0274c9be5430dacda8be2335c21a4 |
| institution | Kabale University |
| issn | 1573-4838 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Springer |
| record_format | Article |
| series | Journal of Materials Science: Materials in Medicine |
| spelling | doaj-art-87f0274c9be5430dacda8be2335c21a42025-01-26T12:12:49ZengSpringerJournal of Materials Science: Materials in Medicine1573-48382025-01-0136112010.1007/s10856-024-06853-4Anodized Ti6Al4V-ELI, electroplated with copper is bactericidal against Staphylococcus aureus and enhances macrophage phagocytosisPaula Milena Giraldo-Osorno0Adam Benedict Turner1Sebastião Mollet Barros2Robin Büscher3Simone Guttau4Farah Asa’ad5Margarita Trobos6Anders Palmquist7Department of Biomaterials, Institute of Clinical Sciences, Sahlgrenska Academy, University of GothenburgDepartment of Biomaterials, Institute of Clinical Sciences, Sahlgrenska Academy, University of GothenburgStryker Trauma GmbhStryker Trauma GmbhStryker Trauma GmbhDepartment of Biomaterials, Institute of Clinical Sciences, Sahlgrenska Academy, University of GothenburgDepartment of Biomaterials, Institute of Clinical Sciences, Sahlgrenska Academy, University of GothenburgDepartment of Biomaterials, Institute of Clinical Sciences, Sahlgrenska Academy, University of GothenburgAbstract Implants aim to restore skeletal dysfunction associated with ageing and trauma, yet infection and ineffective immune responses can lead to failure. This project characterized the microbiological and host cell responses to titanium alloy with or without electroplated metallic copper. Bacterial viability counting and scanning electron microscopy quantified and visualized the direct and indirect bactericidal effects of the Cu-electroplated titanium (Cu-Ep-Ti) against two different Staphylococcus aureus strains. Human THP-1 macrophage adhesion and viability was analyzed, along with phagocytosis. Results showed potent antimicrobial activity alongside promising host-immunomodulatory properties. Direct and indirect exposure to Cu-Ep-Ti produced potent bactericidal effects resulting in 94–100% reductions in bacterial viability at 24 h, with complete eradication in some cases. As expected, cytotoxicity was observed in THP-1 macrophages without media exchange, though when media was exchanged at 8, 24 and 48 h cell viability was equivalent to Control-Ti. Interestingly macrophages adhered to the copper material or grown in the presence of copper ions showed 7-fold increase in phagocytosis of S. aureus bioparticles compared to Control-Ti, suggesting a dual bactericidal and host immunomodulatory mechanism. In conclusion, this Cu-electroplated Ti biomaterial can limit bacterial contamination on the implant surface, whilst simultaneously promoting a beneficial antimicrobial immune response. Graphical Abstracthttps://doi.org/10.1007/s10856-024-06853-4 |
| spellingShingle | Paula Milena Giraldo-Osorno Adam Benedict Turner Sebastião Mollet Barros Robin Büscher Simone Guttau Farah Asa’ad Margarita Trobos Anders Palmquist Anodized Ti6Al4V-ELI, electroplated with copper is bactericidal against Staphylococcus aureus and enhances macrophage phagocytosis Journal of Materials Science: Materials in Medicine |
| title | Anodized Ti6Al4V-ELI, electroplated with copper is bactericidal against Staphylococcus aureus and enhances macrophage phagocytosis |
| title_full | Anodized Ti6Al4V-ELI, electroplated with copper is bactericidal against Staphylococcus aureus and enhances macrophage phagocytosis |
| title_fullStr | Anodized Ti6Al4V-ELI, electroplated with copper is bactericidal against Staphylococcus aureus and enhances macrophage phagocytosis |
| title_full_unstemmed | Anodized Ti6Al4V-ELI, electroplated with copper is bactericidal against Staphylococcus aureus and enhances macrophage phagocytosis |
| title_short | Anodized Ti6Al4V-ELI, electroplated with copper is bactericidal against Staphylococcus aureus and enhances macrophage phagocytosis |
| title_sort | anodized ti6al4v eli electroplated with copper is bactericidal against staphylococcus aureus and enhances macrophage phagocytosis |
| url | https://doi.org/10.1007/s10856-024-06853-4 |
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