Transcriptomic analysis of Medicago truncatula calli with MtWOX9-1 overexpression
Somatic embryogenesis (SE) is the development of embryo-like structures from somatic plant tissues. This process rarely can be observed in nature, but for many plant species, in vitro protocols are developed, which allow to obtain somatic embryos formation directly from tissues of plant explant or f...
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| Format: | Article |
| Language: | English |
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Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2019-10-01
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| Series: | Вавиловский журнал генетики и селекции |
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| Online Access: | https://vavilov.elpub.ru/jour/article/view/2259 |
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| author | V. E. Tvorogova E. Y. Krasnoperova A. A. Kudriashov K. A. Kuznetsova E. A. Potsenkovskaya Y. A. Fedorova L. A. Lutova |
| author_facet | V. E. Tvorogova E. Y. Krasnoperova A. A. Kudriashov K. A. Kuznetsova E. A. Potsenkovskaya Y. A. Fedorova L. A. Lutova |
| author_sort | V. E. Tvorogova |
| collection | DOAJ |
| description | Somatic embryogenesis (SE) is the development of embryo-like structures from somatic plant tissues. This process rarely can be observed in nature, but for many plant species, in vitro protocols are developed, which allow to obtain somatic embryos formation directly from tissues of plant explant or from the embryogenic callus. SE is widely used for plant propagation and transformation; therefore, the search for SE stimulators and revealing of the mechanisms of their functioning are very important for biotechnology. Among the SE regulators, proteins of the WOX family play significant roles. WOX (WUSCHEL-RELATED HOMEOBOX) is a homeodomain-containing transcription factor family. Different WOX genes function in different plant organs and tissues, maintaining meristem activity and regulating cell proliferation and differentiation. Recently, we have shown that transcription factor MtWOX9-1, belonging to the WOX family, can stimulate SE in the Medicago truncatula callus culture. In this research, transcriptomic analysis of highly embryogenic calli with MtWOX9-1 overexpression was performed in comparison to wildtype calli. It was shown that MtWOX9-1 overexpression led to the activation of several groups of genes, including genes related to cell division, tissue differentiation, and seed development. Enriched GO pathways included several groups related to histone methyltransferase activity as well as DNA methylation and chromatin binding, suggesting major epigenetic changes that occur in call overexpressing MtWOX9-1. Using Medicago Truncatula Gene Expression Atlas, we also identified a group of genes coding for transcription factors that were both coexpressed with MtWOX9-1 in different plant organs and differentially expressed in our samples. These genes are putative targets of MtWOX9-1, and they may act in the same pathway with this regulator during SE. |
| format | Article |
| id | doaj-art-8763834032e34a56a3cc7bf5298d95c6 |
| institution | Kabale University |
| issn | 2500-3259 |
| language | English |
| publishDate | 2019-10-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj-art-8763834032e34a56a3cc7bf5298d95c62025-02-01T09:58:08ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592019-10-0123669169910.18699/VJ19.542962Transcriptomic analysis of Medicago truncatula calli with MtWOX9-1 overexpressionV. E. Tvorogova0E. Y. Krasnoperova1A. A. Kudriashov2K. A. Kuznetsova3E. A. Potsenkovskaya4Y. A. Fedorova5L. A. Lutova6St. Petersburg State University, Department of Genetics and BiotechnologySt. Petersburg State University, Department of Genetics and BiotechnologySt. Petersburg State University, Department of Genetics and BiotechnologySt. Petersburg State University, Department of Genetics and BiotechnologySt. Petersburg State University, Department of Genetics and BiotechnologySt. Petersburg State University, Department of Genetics and BiotechnologySt. Petersburg State University, Department of Genetics and BiotechnologySomatic embryogenesis (SE) is the development of embryo-like structures from somatic plant tissues. This process rarely can be observed in nature, but for many plant species, in vitro protocols are developed, which allow to obtain somatic embryos formation directly from tissues of plant explant or from the embryogenic callus. SE is widely used for plant propagation and transformation; therefore, the search for SE stimulators and revealing of the mechanisms of their functioning are very important for biotechnology. Among the SE regulators, proteins of the WOX family play significant roles. WOX (WUSCHEL-RELATED HOMEOBOX) is a homeodomain-containing transcription factor family. Different WOX genes function in different plant organs and tissues, maintaining meristem activity and regulating cell proliferation and differentiation. Recently, we have shown that transcription factor MtWOX9-1, belonging to the WOX family, can stimulate SE in the Medicago truncatula callus culture. In this research, transcriptomic analysis of highly embryogenic calli with MtWOX9-1 overexpression was performed in comparison to wildtype calli. It was shown that MtWOX9-1 overexpression led to the activation of several groups of genes, including genes related to cell division, tissue differentiation, and seed development. Enriched GO pathways included several groups related to histone methyltransferase activity as well as DNA methylation and chromatin binding, suggesting major epigenetic changes that occur in call overexpressing MtWOX9-1. Using Medicago Truncatula Gene Expression Atlas, we also identified a group of genes coding for transcription factors that were both coexpressed with MtWOX9-1 in different plant organs and differentially expressed in our samples. These genes are putative targets of MtWOX9-1, and they may act in the same pathway with this regulator during SE.https://vavilov.elpub.ru/jour/article/view/2259somatic embryogenesismedicago truncatulaplant regenerationtranscription factorstranscriptomic analysis |
| spellingShingle | V. E. Tvorogova E. Y. Krasnoperova A. A. Kudriashov K. A. Kuznetsova E. A. Potsenkovskaya Y. A. Fedorova L. A. Lutova Transcriptomic analysis of Medicago truncatula calli with MtWOX9-1 overexpression Вавиловский журнал генетики и селекции somatic embryogenesis medicago truncatula plant regeneration transcription factors transcriptomic analysis |
| title | Transcriptomic analysis of Medicago truncatula calli with MtWOX9-1 overexpression |
| title_full | Transcriptomic analysis of Medicago truncatula calli with MtWOX9-1 overexpression |
| title_fullStr | Transcriptomic analysis of Medicago truncatula calli with MtWOX9-1 overexpression |
| title_full_unstemmed | Transcriptomic analysis of Medicago truncatula calli with MtWOX9-1 overexpression |
| title_short | Transcriptomic analysis of Medicago truncatula calli with MtWOX9-1 overexpression |
| title_sort | transcriptomic analysis of medicago truncatula calli with mtwox9 1 overexpression |
| topic | somatic embryogenesis medicago truncatula plant regeneration transcription factors transcriptomic analysis |
| url | https://vavilov.elpub.ru/jour/article/view/2259 |
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