Oxidative stress in critically ill neonatal foals
Abstract Background Oxidative injury occurs in septic people, but the role of oxidative stress and antioxidants has rarely been evaluated in foals. Objectives/Hypothesis To measure reactive oxygen species (ROS), biomarkers of oxidative injury, and antioxidants in neonatal foals. We hypothesized that...
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| Format: | Article |
| Language: | English |
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Wiley
2025-01-01
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| Series: | Journal of Veterinary Internal Medicine |
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| Online Access: | https://doi.org/10.1111/jvim.17297 |
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| _version_ | 1832584137791242240 |
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| author | David Wong Dipak Kumar Sahoo Cosette Faivre Jamie Kopper Katie Dersh Theresa Beachler Melissa Esser |
| author_facet | David Wong Dipak Kumar Sahoo Cosette Faivre Jamie Kopper Katie Dersh Theresa Beachler Melissa Esser |
| author_sort | David Wong |
| collection | DOAJ |
| description | Abstract Background Oxidative injury occurs in septic people, but the role of oxidative stress and antioxidants has rarely been evaluated in foals. Objectives/Hypothesis To measure reactive oxygen species (ROS), biomarkers of oxidative injury, and antioxidants in neonatal foals. We hypothesized that ill foals would have higher blood concentrations of ROS and biomarkers of oxidative injury and lower concentrations of antioxidants compared to healthy foals. Animals Seventy‐two hospitalized and 21 healthy neonatal foals. Methods Prospective cohort study. Reactive oxygen species (hydrogen peroxide [H2O2]), biomarkers of oxidative injury (malondialdehyde [MDA], protein carbonyl), and antioxidants (superoxide dismutase [SOD], catalase [CAT], glutathione, and glutathione reductase [GR] and peroxidase [GPx]) were measured from foals at admission. Measured variables were compared between healthy and ill foals using a 1‐way ANOVA by Tukey's multiple comparisons test. Results Ill foals (n = 51) had significantly higher mean concentrations of H2O2 (healthy 2.6 ± 1.4 nmol/mL, ill 6.8 ± 4.6 L nmol/mL; 95% CI), MDA (healthy 31.2 ± 14.4 nmol/mL, ill 114.3 ± 94.0 nmol/mL; 95% CI), and protein carbonyl (healthy 0.07 ± 0.01 nmol/mg protein, ill 0.12 ± 0.02 nmol/mg protein, 95% CI). Significant lower CAT (healthy 0.4 ± 0.3 mU/mg protein, ill 0.02 ± 0.02 mU/mg protein, 95% CI), glutathione (healthy 238.5 ± 101.9 μg/mL, ill 110.7 ± 37.8 μg/mL, 95% CI; P < .0001), GR (healthy 1.6 ± 1.8 mU/mg protein, ill 0.4 ± 0.5 mU/mg protein, 95% CI), and GPx (healthy 0.01 ± 0.003 mU/mg protein, ill 0.007 ± 0.002 mU/mg protein, 95% CI) were also noted. Conclusions and Clinical Importance Oxidative stress and lower antioxidant concentrations occur in ill and bacteremic neonatal foals. These variables should be considered during the treatment of ill foals. |
| format | Article |
| id | doaj-art-8761b541b4934cfdb065186070392924 |
| institution | Kabale University |
| issn | 0891-6640 1939-1676 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Veterinary Internal Medicine |
| spelling | doaj-art-8761b541b4934cfdb0651860703929242025-01-27T15:22:41ZengWileyJournal of Veterinary Internal Medicine0891-66401939-16762025-01-01391n/an/a10.1111/jvim.17297Oxidative stress in critically ill neonatal foalsDavid Wong0Dipak Kumar Sahoo1Cosette Faivre2Jamie Kopper3Katie Dersh4Theresa Beachler5Melissa Esser6Department of Veterinary Clinical Sciences, College of Veterinary Medicine Iowa State University Ames Iowa USADepartment of Veterinary Clinical Sciences, College of Veterinary Medicine Iowa State University Ames Iowa USADepartment of Veterinary Clinical Sciences, College of Veterinary Medicine Iowa State University Ames Iowa USADepartment of Veterinary Clinical Sciences, College of Veterinary Medicine Iowa State University Ames Iowa USADepartment of Veterinary Clinical Sciences, College of Veterinary Medicine Iowa State University Ames Iowa USADepartment of Veterinary Clinical Sciences, College of Veterinary Medicine Iowa State University Ames Iowa USADepartment of Veterinary Clinical Sciences, College of Veterinary Medicine Iowa State University Ames Iowa USAAbstract Background Oxidative injury occurs in septic people, but the role of oxidative stress and antioxidants has rarely been evaluated in foals. Objectives/Hypothesis To measure reactive oxygen species (ROS), biomarkers of oxidative injury, and antioxidants in neonatal foals. We hypothesized that ill foals would have higher blood concentrations of ROS and biomarkers of oxidative injury and lower concentrations of antioxidants compared to healthy foals. Animals Seventy‐two hospitalized and 21 healthy neonatal foals. Methods Prospective cohort study. Reactive oxygen species (hydrogen peroxide [H2O2]), biomarkers of oxidative injury (malondialdehyde [MDA], protein carbonyl), and antioxidants (superoxide dismutase [SOD], catalase [CAT], glutathione, and glutathione reductase [GR] and peroxidase [GPx]) were measured from foals at admission. Measured variables were compared between healthy and ill foals using a 1‐way ANOVA by Tukey's multiple comparisons test. Results Ill foals (n = 51) had significantly higher mean concentrations of H2O2 (healthy 2.6 ± 1.4 nmol/mL, ill 6.8 ± 4.6 L nmol/mL; 95% CI), MDA (healthy 31.2 ± 14.4 nmol/mL, ill 114.3 ± 94.0 nmol/mL; 95% CI), and protein carbonyl (healthy 0.07 ± 0.01 nmol/mg protein, ill 0.12 ± 0.02 nmol/mg protein, 95% CI). Significant lower CAT (healthy 0.4 ± 0.3 mU/mg protein, ill 0.02 ± 0.02 mU/mg protein, 95% CI), glutathione (healthy 238.5 ± 101.9 μg/mL, ill 110.7 ± 37.8 μg/mL, 95% CI; P < .0001), GR (healthy 1.6 ± 1.8 mU/mg protein, ill 0.4 ± 0.5 mU/mg protein, 95% CI), and GPx (healthy 0.01 ± 0.003 mU/mg protein, ill 0.007 ± 0.002 mU/mg protein, 95% CI) were also noted. Conclusions and Clinical Importance Oxidative stress and lower antioxidant concentrations occur in ill and bacteremic neonatal foals. These variables should be considered during the treatment of ill foals.https://doi.org/10.1111/jvim.17297antioxidantascorbic acidoxidative injuryreactive oxygen speciessepsis |
| spellingShingle | David Wong Dipak Kumar Sahoo Cosette Faivre Jamie Kopper Katie Dersh Theresa Beachler Melissa Esser Oxidative stress in critically ill neonatal foals Journal of Veterinary Internal Medicine antioxidant ascorbic acid oxidative injury reactive oxygen species sepsis |
| title | Oxidative stress in critically ill neonatal foals |
| title_full | Oxidative stress in critically ill neonatal foals |
| title_fullStr | Oxidative stress in critically ill neonatal foals |
| title_full_unstemmed | Oxidative stress in critically ill neonatal foals |
| title_short | Oxidative stress in critically ill neonatal foals |
| title_sort | oxidative stress in critically ill neonatal foals |
| topic | antioxidant ascorbic acid oxidative injury reactive oxygen species sepsis |
| url | https://doi.org/10.1111/jvim.17297 |
| work_keys_str_mv | AT davidwong oxidativestressincriticallyillneonatalfoals AT dipakkumarsahoo oxidativestressincriticallyillneonatalfoals AT cosettefaivre oxidativestressincriticallyillneonatalfoals AT jamiekopper oxidativestressincriticallyillneonatalfoals AT katiedersh oxidativestressincriticallyillneonatalfoals AT theresabeachler oxidativestressincriticallyillneonatalfoals AT melissaesser oxidativestressincriticallyillneonatalfoals |