A Simple and Sensitive HPLC Method for Simultaneous Analysis of Nabumetone and Paracetamol in Pharmaceutical Formulations
This paper describes a high-performance liquid chromatographic method for simultaneous estimation of nabumetone and paracetamol in binary mixture. The method was based on RP-HPLC separation and quantitation of the two drugs on hypersil C-18 column (250 mm × 4.6 mm) using a mobile phase consisting of...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2011-01-01
|
| Series: | E-Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2011/607069 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832548472592531456 |
|---|---|
| author | Prafulla Kumar Sahu M. Mathrusri Annapurna Dillipkumar Sahoo |
| author_facet | Prafulla Kumar Sahu M. Mathrusri Annapurna Dillipkumar Sahoo |
| author_sort | Prafulla Kumar Sahu |
| collection | DOAJ |
| description | This paper describes a high-performance liquid chromatographic method for simultaneous estimation of nabumetone and paracetamol in binary mixture. The method was based on RP-HPLC separation and quantitation of the two drugs on hypersil C-18 column (250 mm × 4.6 mm) using a mobile phase consisting of acetonitrile and 0.05% aqueous acetic acid (70:30v/v) at flow rate of 1 mL min-1. Quantitation was achieved with PDA detector at 238 nm based on peak area with linear calibration curves at concentration ranges 5-25 µg mL-1 for both the drugs. Naproxen sodium was used as internal standard. The method has been successively applied to pharmaceutical formulation. No chromatographic interference from the tablet excipients was found. The method was validated in terms of precision, robustness, recovery and limits of detection and quantitation. The intra and inter-day precision and accuracy values were in the acceptance range as per ICH guidelines. |
| format | Article |
| id | doaj-art-8754fd26fdfa42169dae78309e45b463 |
| institution | Kabale University |
| issn | 0973-4945 2090-9810 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | E-Journal of Chemistry |
| spelling | doaj-art-8754fd26fdfa42169dae78309e45b4632025-02-03T06:14:05ZengWileyE-Journal of Chemistry0973-49452090-98102011-01-018S1S41S4610.1155/2011/607069A Simple and Sensitive HPLC Method for Simultaneous Analysis of Nabumetone and Paracetamol in Pharmaceutical FormulationsPrafulla Kumar Sahu0M. Mathrusri Annapurna1Dillipkumar Sahoo2Department of Pharmaceutical Analysis & Quality Assurance, Nimra College of Pharmacy, Nimra Nagar, Ibrahimpatnam, Vijayawada, Andhra Pradesh, 521456, IndiaDepartment of Pharmaceutical Analysis Roland Institute of Pharmaceutical Sciences Khodasing, Berhampur, Ganjam, Orissa, 760010, IndiaDepartment of Pharmaceutical Analysis Roland Institute of Pharmaceutical Sciences Khodasing, Berhampur, Ganjam, Orissa, 760010, IndiaThis paper describes a high-performance liquid chromatographic method for simultaneous estimation of nabumetone and paracetamol in binary mixture. The method was based on RP-HPLC separation and quantitation of the two drugs on hypersil C-18 column (250 mm × 4.6 mm) using a mobile phase consisting of acetonitrile and 0.05% aqueous acetic acid (70:30v/v) at flow rate of 1 mL min-1. Quantitation was achieved with PDA detector at 238 nm based on peak area with linear calibration curves at concentration ranges 5-25 µg mL-1 for both the drugs. Naproxen sodium was used as internal standard. The method has been successively applied to pharmaceutical formulation. No chromatographic interference from the tablet excipients was found. The method was validated in terms of precision, robustness, recovery and limits of detection and quantitation. The intra and inter-day precision and accuracy values were in the acceptance range as per ICH guidelines.http://dx.doi.org/10.1155/2011/607069 |
| spellingShingle | Prafulla Kumar Sahu M. Mathrusri Annapurna Dillipkumar Sahoo A Simple and Sensitive HPLC Method for Simultaneous Analysis of Nabumetone and Paracetamol in Pharmaceutical Formulations E-Journal of Chemistry |
| title | A Simple and Sensitive HPLC Method for Simultaneous Analysis of Nabumetone and Paracetamol in Pharmaceutical Formulations |
| title_full | A Simple and Sensitive HPLC Method for Simultaneous Analysis of Nabumetone and Paracetamol in Pharmaceutical Formulations |
| title_fullStr | A Simple and Sensitive HPLC Method for Simultaneous Analysis of Nabumetone and Paracetamol in Pharmaceutical Formulations |
| title_full_unstemmed | A Simple and Sensitive HPLC Method for Simultaneous Analysis of Nabumetone and Paracetamol in Pharmaceutical Formulations |
| title_short | A Simple and Sensitive HPLC Method for Simultaneous Analysis of Nabumetone and Paracetamol in Pharmaceutical Formulations |
| title_sort | simple and sensitive hplc method for simultaneous analysis of nabumetone and paracetamol in pharmaceutical formulations |
| url | http://dx.doi.org/10.1155/2011/607069 |
| work_keys_str_mv | AT prafullakumarsahu asimpleandsensitivehplcmethodforsimultaneousanalysisofnabumetoneandparacetamolinpharmaceuticalformulations AT mmathrusriannapurna asimpleandsensitivehplcmethodforsimultaneousanalysisofnabumetoneandparacetamolinpharmaceuticalformulations AT dillipkumarsahoo asimpleandsensitivehplcmethodforsimultaneousanalysisofnabumetoneandparacetamolinpharmaceuticalformulations AT prafullakumarsahu simpleandsensitivehplcmethodforsimultaneousanalysisofnabumetoneandparacetamolinpharmaceuticalformulations AT mmathrusriannapurna simpleandsensitivehplcmethodforsimultaneousanalysisofnabumetoneandparacetamolinpharmaceuticalformulations AT dillipkumarsahoo simpleandsensitivehplcmethodforsimultaneousanalysisofnabumetoneandparacetamolinpharmaceuticalformulations |