Therapy of Prostate Cancer by Nanoyam Polysaccharide
We evaluated the effect and mechanism of yam polysaccharide on the proliferation of the prostatic cancer cell line and tumor-bearing mice. The effect of nanoyam polysaccharide on prostatic cancer cell line PC-3 was measured using the scratch adhesion test and flow cytometry. The growth effect induce...
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| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | International Journal of Polymer Science |
| Online Access: | http://dx.doi.org/10.1155/2019/9543526 |
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| _version_ | 1832551961903235072 |
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| author | Cheng Peng Bo Han Baishan Wang Gaoyang Zhao Fang Yuan Yeyu Zhao Liang Liang Dingge Liu Zhaohui Zhai Yanping Shen |
| author_facet | Cheng Peng Bo Han Baishan Wang Gaoyang Zhao Fang Yuan Yeyu Zhao Liang Liang Dingge Liu Zhaohui Zhai Yanping Shen |
| author_sort | Cheng Peng |
| collection | DOAJ |
| description | We evaluated the effect and mechanism of yam polysaccharide on the proliferation of the prostatic cancer cell line and tumor-bearing mice. The effect of nanoyam polysaccharide on prostatic cancer cell line PC-3 was measured using the scratch adhesion test and flow cytometry. The growth effect induced by nanoyam polysaccharide was detected with the CCK-8 test. The levels of caspase-3 protein were determined with Western blot. In our data, nanoyam polysaccharide presented inhibitory effect on the proliferation of PC-3. The scratch adhesion test showed that the rate of wound healing in the intervention group was significantly lower than that in the control group (p<0.05). Flow cytometry assay showed that, after treatment with nanoyam polysaccharide, the apoptosis rate in the intervention group was significantly lower than that in the control group (45.8%±2.6%, 25.8%±3.1%; p<0.05). Western blot assay showed upregulated levels of caspase-3 in the intervention group, compared to the control group (p<0.05). Our results suggested that nanoyam polysaccharide strongly suppressed the growth of prostatic cancer by inducing the overexpression of caspase-3 and may be a potent anticancer strategy. |
| format | Article |
| id | doaj-art-87548debc35c46e9b83336a21e20ae19 |
| institution | Kabale University |
| issn | 1687-9422 1687-9430 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Polymer Science |
| spelling | doaj-art-87548debc35c46e9b83336a21e20ae192025-02-03T06:00:03ZengWileyInternational Journal of Polymer Science1687-94221687-94302019-01-01201910.1155/2019/95435269543526Therapy of Prostate Cancer by Nanoyam PolysaccharideCheng Peng0Bo Han1Baishan Wang2Gaoyang Zhao3Fang Yuan4Yeyu Zhao5Liang Liang6Dingge Liu7Zhaohui Zhai8Yanping Shen9Department of Clinical Laboratory, The Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Shenyang, Liaoning 110032, ChinaDepartment of Clinical Laboratory, The Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Shenyang, Liaoning 110032, ChinaDepartment of Clinical Laboratory, The Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Shenyang, Liaoning 110032, ChinaDepartment of Clinical Laboratory, The Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Shenyang, Liaoning 110032, ChinaDepartment of Nephrology, The Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Shenyang, Liaoning 110032, ChinaDepartment of Rheumatology and Immunology, The Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Shenyang, Liaoning 110032, ChinaDepartment of Nephrology, The Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Shenyang, Liaoning 110032, ChinaMedical College of Soochow University, Suzhou, Jiangsu 215123, ChinaDepartment of Plastic Surgery, Plastic Surgery Hospital of Weifang Medical University, Weifang, Shandong 261042, ChinaDepartment of Nephrology, The First Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215006, ChinaWe evaluated the effect and mechanism of yam polysaccharide on the proliferation of the prostatic cancer cell line and tumor-bearing mice. The effect of nanoyam polysaccharide on prostatic cancer cell line PC-3 was measured using the scratch adhesion test and flow cytometry. The growth effect induced by nanoyam polysaccharide was detected with the CCK-8 test. The levels of caspase-3 protein were determined with Western blot. In our data, nanoyam polysaccharide presented inhibitory effect on the proliferation of PC-3. The scratch adhesion test showed that the rate of wound healing in the intervention group was significantly lower than that in the control group (p<0.05). Flow cytometry assay showed that, after treatment with nanoyam polysaccharide, the apoptosis rate in the intervention group was significantly lower than that in the control group (45.8%±2.6%, 25.8%±3.1%; p<0.05). Western blot assay showed upregulated levels of caspase-3 in the intervention group, compared to the control group (p<0.05). Our results suggested that nanoyam polysaccharide strongly suppressed the growth of prostatic cancer by inducing the overexpression of caspase-3 and may be a potent anticancer strategy.http://dx.doi.org/10.1155/2019/9543526 |
| spellingShingle | Cheng Peng Bo Han Baishan Wang Gaoyang Zhao Fang Yuan Yeyu Zhao Liang Liang Dingge Liu Zhaohui Zhai Yanping Shen Therapy of Prostate Cancer by Nanoyam Polysaccharide International Journal of Polymer Science |
| title | Therapy of Prostate Cancer by Nanoyam Polysaccharide |
| title_full | Therapy of Prostate Cancer by Nanoyam Polysaccharide |
| title_fullStr | Therapy of Prostate Cancer by Nanoyam Polysaccharide |
| title_full_unstemmed | Therapy of Prostate Cancer by Nanoyam Polysaccharide |
| title_short | Therapy of Prostate Cancer by Nanoyam Polysaccharide |
| title_sort | therapy of prostate cancer by nanoyam polysaccharide |
| url | http://dx.doi.org/10.1155/2019/9543526 |
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