Diagnosis of brain death in wistar rats at different levels of death induction [version 2; peer review: 2 approved]
Objective This study aimed to evaluate hematologic, biochemical, and gasometric parameters in Wistar rats to better understand brain death parameters and reduce early misdiagnoses. Methods Fifteen adult male Wistar rats (Rattus norvergicus; HanUnib: WH) were randomly distributed into three groups of...
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F1000 Research Ltd
2025-05-01
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| author | Noeme Sousa Rocha Fernanda Barthelson Carvalho de Moura Gabriela Abreu Botelho Natália Camargo Faraldo Fernanda de Freitas Alves Vieira Ana Beatriz de Souza da Silva Nadia Yumi Yamamo dos Santos Paola Alejandra Montenegro Cuellar Fernando Carmona Dinau Natália Freitas de Souza Giovanna Farina Panebianco Júlia Soares Bodaneze Rogerio Antonio de Oliveira Fernanda Zuliani Nayara Maria Gil Mazzante |
| author_facet | Noeme Sousa Rocha Fernanda Barthelson Carvalho de Moura Gabriela Abreu Botelho Natália Camargo Faraldo Fernanda de Freitas Alves Vieira Ana Beatriz de Souza da Silva Nadia Yumi Yamamo dos Santos Paola Alejandra Montenegro Cuellar Fernando Carmona Dinau Natália Freitas de Souza Giovanna Farina Panebianco Júlia Soares Bodaneze Rogerio Antonio de Oliveira Fernanda Zuliani Nayara Maria Gil Mazzante |
| author_sort | Noeme Sousa Rocha |
| collection | DOAJ |
| description | Objective This study aimed to evaluate hematologic, biochemical, and gasometric parameters in Wistar rats to better understand brain death parameters and reduce early misdiagnoses. Methods Fifteen adult male Wistar rats (Rattus norvergicus; HanUnib: WH) were randomly distributed into three groups of five animals: the control group (G0) with evaluation performed before brain death, and two groups (G1 and G2) with brain death induced at different times: immediately after induction (G1) and one hour after induction (G2). Venous and arterial blood samples were taken to perform complete blood count, biochemical, and blood gas assays. Samples were taken at specific times based on the group each rat belonged to. Results Statistically significant mean values were observed (P<0,05) for segmented cells (G1>G2 and G0>G2), monocytes (G2>G1 and G0>G1), creatinine (G2>G0), aspartate aminotransferase (G1>G0), potassium (G2>G0), and bicarbonate (G0>G1). Discussion Furthermore, brain death showed a unique response in each organism, complicating its precise determination even more. |
| format | Article |
| id | doaj-art-875234e9d6cd4e73a7ec8c83892ef4a2 |
| institution | DOAJ |
| issn | 2046-1402 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | F1000 Research Ltd |
| record_format | Article |
| series | F1000Research |
| spelling | doaj-art-875234e9d6cd4e73a7ec8c83892ef4a22025-08-20T03:09:59ZengF1000 Research LtdF1000Research2046-14022025-05-011310.12688/f1000research.157233.2181285Diagnosis of brain death in wistar rats at different levels of death induction [version 2; peer review: 2 approved]Noeme Sousa Rocha0Fernanda Barthelson Carvalho de Moura1https://orcid.org/0000-0002-8153-2038Gabriela Abreu Botelho2Natália Camargo Faraldo3Fernanda de Freitas Alves Vieira4Ana Beatriz de Souza da Silva5Nadia Yumi Yamamo dos Santos6Paola Alejandra Montenegro Cuellar7Fernando Carmona Dinau8Natália Freitas de Souza9Giovanna Farina Panebianco10Júlia Soares Bodaneze11Rogerio Antonio de Oliveira12Fernanda Zuliani13Nayara Maria Gil Mazzante14Department of Veterinary Clinics, School of Veterinary Medicine and Animal Science, São Paulo State University - Botucatu Campus, Botucatu, State of São Paulo, BrazilDepartment of Veterinary Clinics, School of Veterinary Medicine and Animal Science, São Paulo State University - Botucatu Campus, Botucatu, State of São Paulo, BrazilDepartment of Veterinary Clinics, School of Veterinary Medicine and Animal Science, São Paulo State University - Botucatu Campus, Botucatu, State of São Paulo, BrazilDepartment of Veterinary Surgery and Reproduction, School of Veterinary Medicine and Animal Science, São Paulo State University, Botucatu, State of São Paulo, BrazilDepartment of Veterinary Clinics, School of Veterinary Medicine and Animal Science, São Paulo State University - Botucatu Campus, Botucatu, State of São Paulo, BrazilDepartment of Veterinary Clinics, School of Veterinary Medicine and Animal Science, São Paulo State University - Botucatu Campus, Botucatu, State of São Paulo, BrazilDepartment of Veterinary Clinics, School of Veterinary Medicine and Animal Science, São Paulo State University - Botucatu Campus, Botucatu, State of São Paulo, BrazilDepartment of Veterinary Clinics, School of Veterinary Medicine and Animal Science, São Paulo State University - Botucatu Campus, Botucatu, State of São Paulo, BrazilDepartment of Veterinary Clinics, School of Veterinary Medicine and Animal Science, São Paulo State University - Botucatu Campus, Botucatu, State of São Paulo, BrazilDepartment of Veterinary Clinics, School of Veterinary Medicine and Animal Science, São Paulo State University - Botucatu Campus, Botucatu, State of São Paulo, BrazilDepartment of Veterinary Clinics, School of Veterinary Medicine and Animal Science, São Paulo State University - Botucatu Campus, Botucatu, State of São Paulo, BrazilDepartment of Veterinary Clinics, School of Veterinary Medicine and Animal Science, São Paulo State University - Botucatu Campus, Botucatu, State of São Paulo, BrazilDepartment of Biostatistics, Institute of Biosciences, São Paulo State University (UNESP), Botucatu, State of São Paulo, BrazilDepartment of Veterinary Clinics, School of Veterinary Medicine and Animal Science, São Paulo State University - Botucatu Campus, Botucatu, State of São Paulo, BrazilDepartment of Veterinary Clinics, School of Veterinary Medicine and Animal Science, São Paulo State University - Botucatu Campus, Botucatu, State of São Paulo, BrazilObjective This study aimed to evaluate hematologic, biochemical, and gasometric parameters in Wistar rats to better understand brain death parameters and reduce early misdiagnoses. Methods Fifteen adult male Wistar rats (Rattus norvergicus; HanUnib: WH) were randomly distributed into three groups of five animals: the control group (G0) with evaluation performed before brain death, and two groups (G1 and G2) with brain death induced at different times: immediately after induction (G1) and one hour after induction (G2). Venous and arterial blood samples were taken to perform complete blood count, biochemical, and blood gas assays. Samples were taken at specific times based on the group each rat belonged to. Results Statistically significant mean values were observed (P<0,05) for segmented cells (G1>G2 and G0>G2), monocytes (G2>G1 and G0>G1), creatinine (G2>G0), aspartate aminotransferase (G1>G0), potassium (G2>G0), and bicarbonate (G0>G1). Discussion Furthermore, brain death showed a unique response in each organism, complicating its precise determination even more.https://f1000research.com/articles/13-1490/v2brain death; hematologic analysis; Wistar rats eng |
| spellingShingle | Noeme Sousa Rocha Fernanda Barthelson Carvalho de Moura Gabriela Abreu Botelho Natália Camargo Faraldo Fernanda de Freitas Alves Vieira Ana Beatriz de Souza da Silva Nadia Yumi Yamamo dos Santos Paola Alejandra Montenegro Cuellar Fernando Carmona Dinau Natália Freitas de Souza Giovanna Farina Panebianco Júlia Soares Bodaneze Rogerio Antonio de Oliveira Fernanda Zuliani Nayara Maria Gil Mazzante Diagnosis of brain death in wistar rats at different levels of death induction [version 2; peer review: 2 approved] F1000Research brain death; hematologic analysis; Wistar rats eng |
| title | Diagnosis of brain death in wistar rats at different levels of death induction [version 2; peer review: 2 approved] |
| title_full | Diagnosis of brain death in wistar rats at different levels of death induction [version 2; peer review: 2 approved] |
| title_fullStr | Diagnosis of brain death in wistar rats at different levels of death induction [version 2; peer review: 2 approved] |
| title_full_unstemmed | Diagnosis of brain death in wistar rats at different levels of death induction [version 2; peer review: 2 approved] |
| title_short | Diagnosis of brain death in wistar rats at different levels of death induction [version 2; peer review: 2 approved] |
| title_sort | diagnosis of brain death in wistar rats at different levels of death induction version 2 peer review 2 approved |
| topic | brain death; hematologic analysis; Wistar rats eng |
| url | https://f1000research.com/articles/13-1490/v2 |
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