Diagnosis of brain death in wistar rats at different levels of death induction [version 2; peer review: 2 approved]

Diagnosis of brain death in wistar rats at different levels of death induction [version 2; peer review: 2 approved]

Objective This study aimed to evaluate hematologic, biochemical, and gasometric parameters in Wistar rats to better understand brain death parameters and reduce early misdiagnoses. Methods Fifteen adult male Wistar rats (Rattus norvergicus; HanUnib: WH) were randomly distributed into three groups of...

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Main Authors: Noeme Sousa Rocha, Fernanda Barthelson Carvalho de Moura, Gabriela Abreu Botelho, Natália Camargo Faraldo, Fernanda de Freitas Alves Vieira, Ana Beatriz de Souza da Silva, Nadia Yumi Yamamo dos Santos, Paola Alejandra Montenegro Cuellar, Fernando Carmona Dinau, Natália Freitas de Souza, Giovanna Farina Panebianco, Júlia Soares Bodaneze, Rogerio Antonio de Oliveira, Fernanda Zuliani, Nayara Maria Gil Mazzante
Format: Article
Language:English
Published: F1000 Research Ltd 2025-05-01
Series:F1000Research
Subjects:
brain death; hematologic analysis; Wistar rats
eng
Online Access:https://f1000research.com/articles/13-1490/v2
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Summary:Objective This study aimed to evaluate hematologic, biochemical, and gasometric parameters in Wistar rats to better understand brain death parameters and reduce early misdiagnoses. Methods Fifteen adult male Wistar rats (Rattus norvergicus; HanUnib: WH) were randomly distributed into three groups of five animals: the control group (G0) with evaluation performed before brain death, and two groups (G1 and G2) with brain death induced at different times: immediately after induction (G1) and one hour after induction (G2). Venous and arterial blood samples were taken to perform complete blood count, biochemical, and blood gas assays. Samples were taken at specific times based on the group each rat belonged to. Results Statistically significant mean values were observed (P<0,05) for segmented cells (G1>G2 and G0>G2), monocytes (G2>G1 and G0>G1), creatinine (G2>G0), aspartate aminotransferase (G1>G0), potassium (G2>G0), and bicarbonate (G0>G1). Discussion Furthermore, brain death showed a unique response in each organism, complicating its precise determination even more.
ISSN:2046-1402

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