Influence of aquaculture practices on microbiota composition and pathogen abundance in pond ecosystems in South China
Pond microbiota play a crucial role in maintaining water quality and the health of aquaculture species. This study aimed to explore the relationship between pond water and sediment microbiota (especially potential pathogens) and physicochemical parameters under different aquaculture conditions. Samp...
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| Language: | English |
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Elsevier
2025-05-01
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| Series: | Water Research X |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589914725000027 |
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| author | Shuhui Niu Chuanlong Li Jun Xie Zhifei Li Kai Zhang Guangjun Wang Yun Xia Jingjing Tian Hongyan Li Wenping Xie Wangbao Gong |
| author_facet | Shuhui Niu Chuanlong Li Jun Xie Zhifei Li Kai Zhang Guangjun Wang Yun Xia Jingjing Tian Hongyan Li Wenping Xie Wangbao Gong |
| author_sort | Shuhui Niu |
| collection | DOAJ |
| description | Pond microbiota play a crucial role in maintaining water quality and the health of aquaculture species. This study aimed to explore the relationship between pond water and sediment microbiota (especially potential pathogens) and physicochemical parameters under different aquaculture conditions. Samples of pond water and sediment were collected from 21 monitoring sites across eastern, western, and northern Guangdong, and the Pearl River Delta in November 2021, March 2022, and July 2022. Microbial structures were analyzed using high-throughput sequencing of the 16S rRNA gene. The results indicated that sediment microbiota distribution was more uniform than that of water microbiota. Additionally, sampling time significantly influenced the uniformity of water microbiota distribution more than that sediment microbiota. Factors such as aquaculture species, culture pattern, NH4+-N, longitude, latitude, total nitrogen (TN), NO3−-N, NO2−-N, and total phosphorus (TP) were significantly correlated with water microbiota structure, and TN, TP, and organic carbon were significantly correlated with sediment microbiota structure. Furthermore, an increase in the NH4+-N concentration in the pond water significantly increased the variety of pathogenic bacteria. Higher nitrogen levels also increased the relative abundance of Mycobacterium in pond water, whereas the culture pattern (freshwater, seawater, brackish, modern captive culture, freshwater factory container aquaculture, or seawater factory culture) and species significantly influenced the relative abundances of Vibrio, Tenacibaculum, Pseudoalteromonas, and Francisella. Additionally, the relative abundance of pathogenic bacteria in the sediment microbiota was significantly higher than that in the water microbiota. Our results suggest that the culture patterns, species, and nitrogen concentrations should be considered when preventing pathogenic bacteria growth in aquaculture waters. |
| format | Article |
| id | doaj-art-8739bc1f8306451cad3ef7628308bf0d |
| institution | Kabale University |
| issn | 2589-9147 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Water Research X |
| spelling | doaj-art-8739bc1f8306451cad3ef7628308bf0d2025-01-23T05:27:37ZengElsevierWater Research X2589-91472025-05-0127100302Influence of aquaculture practices on microbiota composition and pathogen abundance in pond ecosystems in South ChinaShuhui Niu0Chuanlong Li1Jun Xie2Zhifei Li3Kai Zhang4Guangjun Wang5Yun Xia6Jingjing Tian7Hongyan Li8Wenping Xie9Wangbao Gong10Key Laboratory of Tropical and Subtropical Fishery Resource Application and Cultivation, Ministry of Agriculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, Guangdong, 510380, China; Guangdong Ecological Remediation of Aquaculture Pollution Research Center, Guangzhou, Guangdong, 510380, ChinaKey Laboratory of Tropical and Subtropical Fishery Resource Application and Cultivation, Ministry of Agriculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, Guangdong, 510380, China; Guangdong Ecological Remediation of Aquaculture Pollution Research Center, Guangzhou, Guangdong, 510380, ChinaKey Laboratory of Tropical and Subtropical Fishery Resource Application and Cultivation, Ministry of Agriculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, Guangdong, 510380, China; Guangdong Ecological Remediation of Aquaculture Pollution Research Center, Guangzhou, Guangdong, 510380, ChinaKey Laboratory of Tropical and Subtropical Fishery Resource Application and Cultivation, Ministry of Agriculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, Guangdong, 510380, China; Guangdong Ecological Remediation of Aquaculture Pollution Research Center, Guangzhou, Guangdong, 510380, ChinaKey Laboratory of Tropical and Subtropical Fishery Resource Application and Cultivation, Ministry of Agriculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, Guangdong, 510380, China; Guangdong Ecological Remediation of Aquaculture Pollution Research Center, Guangzhou, Guangdong, 510380, ChinaKey Laboratory of Tropical and Subtropical Fishery Resource Application and Cultivation, Ministry of Agriculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, Guangdong, 510380, China; Guangdong Ecological Remediation of Aquaculture Pollution Research Center, Guangzhou, Guangdong, 510380, ChinaKey Laboratory of Tropical and Subtropical Fishery Resource Application and Cultivation, Ministry of Agriculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, Guangdong, 510380, China; Guangdong Ecological Remediation of Aquaculture Pollution Research Center, Guangzhou, Guangdong, 510380, ChinaKey Laboratory of Tropical and Subtropical Fishery Resource Application and Cultivation, Ministry of Agriculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, Guangdong, 510380, China; Guangdong Ecological Remediation of Aquaculture Pollution Research Center, Guangzhou, Guangdong, 510380, ChinaKey Laboratory of Tropical and Subtropical Fishery Resource Application and Cultivation, Ministry of Agriculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, Guangdong, 510380, China; Guangdong Ecological Remediation of Aquaculture Pollution Research Center, Guangzhou, Guangdong, 510380, ChinaKey Laboratory of Tropical and Subtropical Fishery Resource Application and Cultivation, Ministry of Agriculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, Guangdong, 510380, China; Guangdong Ecological Remediation of Aquaculture Pollution Research Center, Guangzhou, Guangdong, 510380, ChinaKey Laboratory of Tropical and Subtropical Fishery Resource Application and Cultivation, Ministry of Agriculture, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, Guangdong, 510380, China; Guangdong Ecological Remediation of Aquaculture Pollution Research Center, Guangzhou, Guangdong, 510380, China; Corresponding author at: No.1, Xingyu Road, Dongjiao street, Liwan District, Guangzhou, Guangdong, 510380, China.Pond microbiota play a crucial role in maintaining water quality and the health of aquaculture species. This study aimed to explore the relationship between pond water and sediment microbiota (especially potential pathogens) and physicochemical parameters under different aquaculture conditions. Samples of pond water and sediment were collected from 21 monitoring sites across eastern, western, and northern Guangdong, and the Pearl River Delta in November 2021, March 2022, and July 2022. Microbial structures were analyzed using high-throughput sequencing of the 16S rRNA gene. The results indicated that sediment microbiota distribution was more uniform than that of water microbiota. Additionally, sampling time significantly influenced the uniformity of water microbiota distribution more than that sediment microbiota. Factors such as aquaculture species, culture pattern, NH4+-N, longitude, latitude, total nitrogen (TN), NO3−-N, NO2−-N, and total phosphorus (TP) were significantly correlated with water microbiota structure, and TN, TP, and organic carbon were significantly correlated with sediment microbiota structure. Furthermore, an increase in the NH4+-N concentration in the pond water significantly increased the variety of pathogenic bacteria. Higher nitrogen levels also increased the relative abundance of Mycobacterium in pond water, whereas the culture pattern (freshwater, seawater, brackish, modern captive culture, freshwater factory container aquaculture, or seawater factory culture) and species significantly influenced the relative abundances of Vibrio, Tenacibaculum, Pseudoalteromonas, and Francisella. Additionally, the relative abundance of pathogenic bacteria in the sediment microbiota was significantly higher than that in the water microbiota. Our results suggest that the culture patterns, species, and nitrogen concentrations should be considered when preventing pathogenic bacteria growth in aquaculture waters.http://www.sciencedirect.com/science/article/pii/S2589914725000027Pond microbiotaAquacultureMicrobiota structurePathogenic bacterium |
| spellingShingle | Shuhui Niu Chuanlong Li Jun Xie Zhifei Li Kai Zhang Guangjun Wang Yun Xia Jingjing Tian Hongyan Li Wenping Xie Wangbao Gong Influence of aquaculture practices on microbiota composition and pathogen abundance in pond ecosystems in South China Water Research X Pond microbiota Aquaculture Microbiota structure Pathogenic bacterium |
| title | Influence of aquaculture practices on microbiota composition and pathogen abundance in pond ecosystems in South China |
| title_full | Influence of aquaculture practices on microbiota composition and pathogen abundance in pond ecosystems in South China |
| title_fullStr | Influence of aquaculture practices on microbiota composition and pathogen abundance in pond ecosystems in South China |
| title_full_unstemmed | Influence of aquaculture practices on microbiota composition and pathogen abundance in pond ecosystems in South China |
| title_short | Influence of aquaculture practices on microbiota composition and pathogen abundance in pond ecosystems in South China |
| title_sort | influence of aquaculture practices on microbiota composition and pathogen abundance in pond ecosystems in south china |
| topic | Pond microbiota Aquaculture Microbiota structure Pathogenic bacterium |
| url | http://www.sciencedirect.com/science/article/pii/S2589914725000027 |
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