Automated Evaluation of Crithidia luciliae Based Indirect Immunofluorescence Tests: A Novel Application of the EUROPattern-Suite Technology
Systemic lupus erythematosus (SLE) is a severe rheumatic autoimmune disease with various clinical manifestations. Anti-dsDNA antibodies are an important immunological hallmark of SLE and their occurrence represents a major criterion for the diagnosis. Among the commonly applied test systems for dete...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2015/742402 |
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| author | Stefan Gerlach Kai Affeldt Lena Pototzki Christopher Krause Jörn Voigt Johanna Fraune Kai Fechner |
| author_facet | Stefan Gerlach Kai Affeldt Lena Pototzki Christopher Krause Jörn Voigt Johanna Fraune Kai Fechner |
| author_sort | Stefan Gerlach |
| collection | DOAJ |
| description | Systemic lupus erythematosus (SLE) is a severe rheumatic autoimmune disease with various clinical manifestations. Anti-dsDNA antibodies are an important immunological hallmark of SLE and their occurrence represents a major criterion for the diagnosis. Among the commonly applied test systems for determination of anti-dsDNA antibodies, the indirect immunofluorescence test (IIFT) using the flagellated kinetoplastida Crithidia luciliae is considered to be highly disease specific at moderate sensitivity. Since IIFT, however, is claimed to be affected by subjective interpretation and a lack of standardization, there has been an increasing demand for automated pattern interpretation of immunofluorescence reactions in recent years. Corresponding platforms are already available for evaluation of anti-nuclear antibody (ANA) IIFT on HEp-2 cells, the recommended “gold standard” for ANA screening in the diagnosis of various systemic rheumatic autoimmune diseases. For one of these systems, the “EUROPattern-Suite” computer-aided immunofluorescence microscopy (CAIFM), automated interpretation of microscopic fluorescence patterns was extended to the Crithidia luciliae based anti-dsDNA IIFT. |
| format | Article |
| id | doaj-art-86c4aad346ff47c49317f68b18ff617a |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-86c4aad346ff47c49317f68b18ff617a2025-02-03T01:21:18ZengWileyJournal of Immunology Research2314-88612314-71562015-01-01201510.1155/2015/742402742402Automated Evaluation of Crithidia luciliae Based Indirect Immunofluorescence Tests: A Novel Application of the EUROPattern-Suite TechnologyStefan Gerlach0Kai Affeldt1Lena Pototzki2Christopher Krause3Jörn Voigt4Johanna Fraune5Kai Fechner6Institute for Experimental Immunology, EUROIMMUN, Seekamp 31, 23560 Lübeck, GermanyInstitute for Experimental Immunology, EUROIMMUN, Seekamp 31, 23560 Lübeck, GermanyInstitute for Experimental Immunology, EUROIMMUN, Seekamp 31, 23560 Lübeck, GermanyInstitute for Experimental Immunology, EUROIMMUN, Seekamp 31, 23560 Lübeck, GermanyInstitute for Experimental Immunology, EUROIMMUN, Seekamp 31, 23560 Lübeck, GermanyInstitute for Experimental Immunology, EUROIMMUN, Seekamp 31, 23560 Lübeck, GermanyInstitute for Experimental Immunology, EUROIMMUN, Seekamp 31, 23560 Lübeck, GermanySystemic lupus erythematosus (SLE) is a severe rheumatic autoimmune disease with various clinical manifestations. Anti-dsDNA antibodies are an important immunological hallmark of SLE and their occurrence represents a major criterion for the diagnosis. Among the commonly applied test systems for determination of anti-dsDNA antibodies, the indirect immunofluorescence test (IIFT) using the flagellated kinetoplastida Crithidia luciliae is considered to be highly disease specific at moderate sensitivity. Since IIFT, however, is claimed to be affected by subjective interpretation and a lack of standardization, there has been an increasing demand for automated pattern interpretation of immunofluorescence reactions in recent years. Corresponding platforms are already available for evaluation of anti-nuclear antibody (ANA) IIFT on HEp-2 cells, the recommended “gold standard” for ANA screening in the diagnosis of various systemic rheumatic autoimmune diseases. For one of these systems, the “EUROPattern-Suite” computer-aided immunofluorescence microscopy (CAIFM), automated interpretation of microscopic fluorescence patterns was extended to the Crithidia luciliae based anti-dsDNA IIFT.http://dx.doi.org/10.1155/2015/742402 |
| spellingShingle | Stefan Gerlach Kai Affeldt Lena Pototzki Christopher Krause Jörn Voigt Johanna Fraune Kai Fechner Automated Evaluation of Crithidia luciliae Based Indirect Immunofluorescence Tests: A Novel Application of the EUROPattern-Suite Technology Journal of Immunology Research |
| title | Automated Evaluation of Crithidia luciliae Based Indirect Immunofluorescence Tests: A Novel Application of the EUROPattern-Suite Technology |
| title_full | Automated Evaluation of Crithidia luciliae Based Indirect Immunofluorescence Tests: A Novel Application of the EUROPattern-Suite Technology |
| title_fullStr | Automated Evaluation of Crithidia luciliae Based Indirect Immunofluorescence Tests: A Novel Application of the EUROPattern-Suite Technology |
| title_full_unstemmed | Automated Evaluation of Crithidia luciliae Based Indirect Immunofluorescence Tests: A Novel Application of the EUROPattern-Suite Technology |
| title_short | Automated Evaluation of Crithidia luciliae Based Indirect Immunofluorescence Tests: A Novel Application of the EUROPattern-Suite Technology |
| title_sort | automated evaluation of crithidia luciliae based indirect immunofluorescence tests a novel application of the europattern suite technology |
| url | http://dx.doi.org/10.1155/2015/742402 |
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