Human keratinocyte sensitivity towards inflammatory cytokines varies with culture time
Proliferating keratinocyte cultures have been reported to synthesize higher concentrations of prostaglandin (PG) E than confluent ones. As interleukin-1 (IL-1) stimulates keratinocyte PGE synthesis we investigated whether the degree of confluency of the keratinocyte culture modified the response of...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wiley
1992-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/S0962935192000589 |
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| _version_ | 1832548472145838080 |
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| author | G. Elliott D. van de Meent J. van Dijk M. Mol |
| author_facet | G. Elliott D. van de Meent J. van Dijk M. Mol |
| author_sort | G. Elliott |
| collection | DOAJ |
| description | Proliferating keratinocyte cultures have been reported to synthesize higher concentrations of prostaglandin (PG) E than confluent ones. As interleukin-1 (IL-1) stimulates keratinocyte PGE synthesis we investigated whether the degree of confluency of the keratinocyte culture modified the response of the cells to IL-1. It was found that IL-1α (100 U/ml) stimulated PGE2 synthesis by proliferating (7 days in culture) but not differentiating (14 days in culture) keratinocytes. Similar effects were observed using tumour necrosis factor-α. Both arachidonic acid (AA) and the calcium ionophore A23187 stimulated PGE2 synthesis by 7 and 14 day cultures although the increase was greatest when 7 day cultures were used. Our data indicate that there is a specific down-regulation of the mechanism(s) by which some inflammatory cytokines stimulate keratinocyte eicosanoid synthesis as cultured keratinocytes begin to differentiate. |
| format | Article |
| id | doaj-art-86c231d21c4f4d52afa4a7ce0f63aaf0 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 1992-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-86c231d21c4f4d52afa4a7ce0f63aaf02025-02-03T06:13:57ZengWileyMediators of Inflammation0962-93511466-18611992-01-011638539010.1155/S0962935192000589Human keratinocyte sensitivity towards inflammatory cytokines varies with culture timeG. Elliott0D. van de Meent1J. van Dijk2M. Mol3Department of Pharmacology, Medical Biological Laboratory, T.N.O., P.O. Box 45, Rijswijk 2280 AA, The NetherlandsDepartment of Pharmacology, Medical Biological Laboratory, T.N.O., P.O. Box 45, Rijswijk 2280 AA, The NetherlandsDepartment of Pharmacology, Erasmus University, P.O. Box 1738, Rotterdam 3000 DR, The NetherlandsDepartment of Pharmacology, Medical Biological Laboratory, T.N.O., P.O. Box 45, Rijswijk 2280 AA, The NetherlandsProliferating keratinocyte cultures have been reported to synthesize higher concentrations of prostaglandin (PG) E than confluent ones. As interleukin-1 (IL-1) stimulates keratinocyte PGE synthesis we investigated whether the degree of confluency of the keratinocyte culture modified the response of the cells to IL-1. It was found that IL-1α (100 U/ml) stimulated PGE2 synthesis by proliferating (7 days in culture) but not differentiating (14 days in culture) keratinocytes. Similar effects were observed using tumour necrosis factor-α. Both arachidonic acid (AA) and the calcium ionophore A23187 stimulated PGE2 synthesis by 7 and 14 day cultures although the increase was greatest when 7 day cultures were used. Our data indicate that there is a specific down-regulation of the mechanism(s) by which some inflammatory cytokines stimulate keratinocyte eicosanoid synthesis as cultured keratinocytes begin to differentiate.http://dx.doi.org/10.1155/S0962935192000589 |
| spellingShingle | G. Elliott D. van de Meent J. van Dijk M. Mol Human keratinocyte sensitivity towards inflammatory cytokines varies with culture time Mediators of Inflammation |
| title | Human keratinocyte sensitivity towards inflammatory cytokines varies with culture time |
| title_full | Human keratinocyte sensitivity towards inflammatory cytokines varies with culture time |
| title_fullStr | Human keratinocyte sensitivity towards inflammatory cytokines varies with culture time |
| title_full_unstemmed | Human keratinocyte sensitivity towards inflammatory cytokines varies with culture time |
| title_short | Human keratinocyte sensitivity towards inflammatory cytokines varies with culture time |
| title_sort | human keratinocyte sensitivity towards inflammatory cytokines varies with culture time |
| url | http://dx.doi.org/10.1155/S0962935192000589 |
| work_keys_str_mv | AT gelliott humankeratinocytesensitivitytowardsinflammatorycytokinesvarieswithculturetime AT dvandemeent humankeratinocytesensitivitytowardsinflammatorycytokinesvarieswithculturetime AT jvandijk humankeratinocytesensitivitytowardsinflammatorycytokinesvarieswithculturetime AT mmol humankeratinocytesensitivitytowardsinflammatorycytokinesvarieswithculturetime |