YgaE Regulates Out Membrane Proteins in Salmonella enterica Serovar Typhi under Hyperosmotic Stress
Salmonella enterica serovar Typhi (S. Typhi) is a human-specific pathogen that causes typhoid fever. In this study, we constructed ΔygaE mutant and a microarray was performed to investigate the role of ygaE in regulation of gene expression changes in response to hyperosmotic stress in S. Typhi. qRT-...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/374276 |
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| author | Min Wang Ping Feng Xun Chen Haifang Zhang Bin Ni Xiaofang Xie Hong Du |
| author_facet | Min Wang Ping Feng Xun Chen Haifang Zhang Bin Ni Xiaofang Xie Hong Du |
| author_sort | Min Wang |
| collection | DOAJ |
| description | Salmonella enterica serovar Typhi (S. Typhi) is a human-specific pathogen that causes typhoid fever. In this study, we constructed ΔygaE mutant and a microarray was performed to investigate the role of ygaE in regulation of gene expression changes in response to hyperosmotic stress in S. Typhi. qRT-PCR was performed to validate the microarray results. Our data indicated that ygaE was the repressor of gab operon in S. Typhi as in Escherichia coli (E. coli), though the sequence of ygaE is totally different from gabC (formerly ygaE) in E. coli. OmpF, OmpC, and OmpA are the most abundant out membrane proteins in S. Typhi. Here we report that YgaE is a repressor of both OmpF and OmpC at the early stage of hyperosmotic stress. Two-dimensional electrophoresis was applied to analyze proteomics of total proteins in wild-type strain and ΔygaE strain and we found that YgaE represses the expression of OmpA at the late stage of hyperosmotic stress. Altogether, our results implied that YgaE regulates out membrane proteins in a time-dependent manner under hyperosmotic stress in S. Typhi. |
| format | Article |
| id | doaj-art-86b087c6a57d4bd7ad3a36779ca89e4d |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-86b087c6a57d4bd7ad3a36779ca89e4d2025-02-03T01:26:09ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/374276374276YgaE Regulates Out Membrane Proteins in Salmonella enterica Serovar Typhi under Hyperosmotic StressMin Wang0Ping Feng1Xun Chen2Haifang Zhang3Bin Ni4Xiaofang Xie5Hong Du6Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou 215004, ChinaClinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou 215004, ChinaClinical Laboratory Center, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing 100091, ChinaDepartment of Biochemistry and Molecular Biology, School of Medical Technology, Jiangsu University, Zhenjiang 212013, ChinaDepartment of Biochemistry and Molecular Biology, School of Medical Technology, Jiangsu University, Zhenjiang 212013, ChinaClinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou 215004, ChinaClinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou 215004, ChinaSalmonella enterica serovar Typhi (S. Typhi) is a human-specific pathogen that causes typhoid fever. In this study, we constructed ΔygaE mutant and a microarray was performed to investigate the role of ygaE in regulation of gene expression changes in response to hyperosmotic stress in S. Typhi. qRT-PCR was performed to validate the microarray results. Our data indicated that ygaE was the repressor of gab operon in S. Typhi as in Escherichia coli (E. coli), though the sequence of ygaE is totally different from gabC (formerly ygaE) in E. coli. OmpF, OmpC, and OmpA are the most abundant out membrane proteins in S. Typhi. Here we report that YgaE is a repressor of both OmpF and OmpC at the early stage of hyperosmotic stress. Two-dimensional electrophoresis was applied to analyze proteomics of total proteins in wild-type strain and ΔygaE strain and we found that YgaE represses the expression of OmpA at the late stage of hyperosmotic stress. Altogether, our results implied that YgaE regulates out membrane proteins in a time-dependent manner under hyperosmotic stress in S. Typhi.http://dx.doi.org/10.1155/2014/374276 |
| spellingShingle | Min Wang Ping Feng Xun Chen Haifang Zhang Bin Ni Xiaofang Xie Hong Du YgaE Regulates Out Membrane Proteins in Salmonella enterica Serovar Typhi under Hyperosmotic Stress The Scientific World Journal |
| title | YgaE Regulates Out Membrane Proteins in Salmonella enterica Serovar Typhi under Hyperosmotic Stress |
| title_full | YgaE Regulates Out Membrane Proteins in Salmonella enterica Serovar Typhi under Hyperosmotic Stress |
| title_fullStr | YgaE Regulates Out Membrane Proteins in Salmonella enterica Serovar Typhi under Hyperosmotic Stress |
| title_full_unstemmed | YgaE Regulates Out Membrane Proteins in Salmonella enterica Serovar Typhi under Hyperosmotic Stress |
| title_short | YgaE Regulates Out Membrane Proteins in Salmonella enterica Serovar Typhi under Hyperosmotic Stress |
| title_sort | ygae regulates out membrane proteins in salmonella enterica serovar typhi under hyperosmotic stress |
| url | http://dx.doi.org/10.1155/2014/374276 |
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