Pumpkin seed oil-loaded chitosan nanoparticles enhance growth, digestive enzymes, and immune response in whiteleg shrimp (Litopenaeus vannamei): Impacts on histopathology and survival against Vibrio parahaemolyticus
The research evaluated the innovative effects of pumpkin seed oil-loaded chitosan nanoparticles (ChNPs/PSO) on the growth, body composition, digestive enzymes, immune response, organ histopathology, and survivability of whiteleg shrimp (Litopenaeus vannamei) juveniles against Vibrio parahaemolyticus...
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Elsevier
2025-03-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513424006872 |
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| author | El-Sayed Hemdan Eissa Wesam Mohamed Salama Samia Elbahnaswy Mai A.M. El-Son Zienab E. Eldin Sara Amer Layla A. Almutairi Fatma Mohamed Ameen Khalil Safia M.A. Bahshwan Mada M. AL-Qurashi Yasmin M. Abd El-Aziz Moaheda E.H. Eissa Aiman A. Alsaegh Roshmon Thomas Mathew El Sayed Taha Rizk |
| author_facet | El-Sayed Hemdan Eissa Wesam Mohamed Salama Samia Elbahnaswy Mai A.M. El-Son Zienab E. Eldin Sara Amer Layla A. Almutairi Fatma Mohamed Ameen Khalil Safia M.A. Bahshwan Mada M. AL-Qurashi Yasmin M. Abd El-Aziz Moaheda E.H. Eissa Aiman A. Alsaegh Roshmon Thomas Mathew El Sayed Taha Rizk |
| author_sort | El-Sayed Hemdan Eissa |
| collection | DOAJ |
| description | The research evaluated the innovative effects of pumpkin seed oil-loaded chitosan nanoparticles (ChNPs/PSO) on the growth, body composition, digestive enzymes, immune response, organ histopathology, and survivability of whiteleg shrimp (Litopenaeus vannamei) juveniles against Vibrio parahaemolyticus. Shrimp were fed basal diets (control diets and 1 g of free nano chitosan; ChNPs) supplemented with ChNPs and different levels of ChNPs/PSO at 1 (ChNPs+PSO1), 1.5 (ChNPs+PSO1.5), and 2 (ChNPs+PSO2) mL/kg for a continuous 60 days period. At the end of the feeding trial, shrimp juveniles were exposed to V. parahaemolyticus. The results showed that dietary inclusion of ChNPs/PSO (1.5 or 2 mL) significantly improved the final weight, feed conversion ratio, weight gain, and specific growth rate of shrimp compared to other groups (P < 0.05). Shrimp fed diets supplemented with 1.5 or 2 mL of ChNPs/PSO demonstrated significantly higher survival rates compared to other groups. These groups also exhibited significant increases in crude protein, ash content, phagocytic index, superoxide dismutase, and lipase levels (P < 0.05). The ChNPs+PSO2 group showed the highest amylase activity (P < 0.05). Furthermore, shrimp in these groups displayed significantly elevated total hemocyte counts, lysozyme activity, and bactericidal capacity (P < 0.05). Besides, a significant increase in phenoloxidase and phagocytic activities was observed in the ChNPs+PSO1 group (P < 0.05). The expression levels of prophenoloxidase (LvproPO) and hemocyanin (LvHc) genes were significantly increased in the hepatopancreas of shrimp fed diets enriched with 1.5 or 2 mL of ChNPs/PSO. The survival rate after infection with V. parahaemolyticus was higher in all ChNPs/PSO-enriched groups compared to the control group. Dietary supplementation with ChNPs/PSO restored the histological damages in the muscle, hepatopancreas, and intestine induced by V. parahaemolyticus infection. In summary, pumpkin seed oil-loaded chitosan nanoparticles (ChNPs/PSO) hold potential as a feed additive for enhancing growth performance, immune ability, and disease resistance against V. parahaemolyticus in Whiteleg shrimp. |
| format | Article |
| id | doaj-art-86af0abcf0e049e9b6fd65b33d706c73 |
| institution | Kabale University |
| issn | 2352-5134 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj-art-86af0abcf0e049e9b6fd65b33d706c732025-02-06T05:12:16ZengElsevierAquaculture Reports2352-51342025-03-0140102599Pumpkin seed oil-loaded chitosan nanoparticles enhance growth, digestive enzymes, and immune response in whiteleg shrimp (Litopenaeus vannamei): Impacts on histopathology and survival against Vibrio parahaemolyticusEl-Sayed Hemdan Eissa0Wesam Mohamed Salama1Samia Elbahnaswy2Mai A.M. El-Son3Zienab E. Eldin4Sara Amer5Layla A. Almutairi6Fatma Mohamed Ameen Khalil7Safia M.A. Bahshwan8Mada M. AL-Qurashi9Yasmin M. Abd El-Aziz10Moaheda E.H. Eissa11Aiman A. Alsaegh12Roshmon Thomas Mathew13El Sayed Taha Rizk14Fish Research Centre, Faculty of Agricultural Environmental Sciences, Arish University, El-Arish, Egypt; Corresponding authors.Zoology Department, Faculty of Science, Tanta university, Tanta, EgyptDepartment of Aquatic Animal Medicine, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt; Corresponding authors.Department of Aquatic Animal Medicine, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, EgyptMaterials Science and Nanotechnology Department, Faculty of Postgraduate Studies for Advanced Sciences, Beni-Suef University, Beni-Suef 62511, EgyptZoology Department, Faculty of Science, Tanta university, Tanta, EgyptDepartment of Biology, College of Science, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi ArabiaKing Khalid University, Applied College, Unit of Health Specialties, Basic Sciences and their Applications, Mohayil, Abha, Asir 61421, Saudi ArabiaBiological Sciences Department, College of Science and Arts, King Abdulaziz University, Rabigh 21911, Saudi ArabiaBiological Sciences Department, College of Science and Arts, King Abdulaziz University, Rabigh 21911, Saudi ArabiaZoology Department, Faculty of Science, Port Said University, Port Said 42526, EgyptBiotechnology Department, Fish Farming and Technology Institute, Suez Canal University, Ismailia 41522, Egypt; Corresponding authors.Department of Clinical Laboratory Sciences, Faculty of Applied Medical Sciences, Umm Al-Qura University, Mecca, Saudi ArabiaFish Resources Research Center, King Faisal University, Hofuf-420, Al-Ahsa 31982, Saudi ArabiaZoology Department, Faculty of Science, Tanta university, Tanta, EgyptThe research evaluated the innovative effects of pumpkin seed oil-loaded chitosan nanoparticles (ChNPs/PSO) on the growth, body composition, digestive enzymes, immune response, organ histopathology, and survivability of whiteleg shrimp (Litopenaeus vannamei) juveniles against Vibrio parahaemolyticus. Shrimp were fed basal diets (control diets and 1 g of free nano chitosan; ChNPs) supplemented with ChNPs and different levels of ChNPs/PSO at 1 (ChNPs+PSO1), 1.5 (ChNPs+PSO1.5), and 2 (ChNPs+PSO2) mL/kg for a continuous 60 days period. At the end of the feeding trial, shrimp juveniles were exposed to V. parahaemolyticus. The results showed that dietary inclusion of ChNPs/PSO (1.5 or 2 mL) significantly improved the final weight, feed conversion ratio, weight gain, and specific growth rate of shrimp compared to other groups (P < 0.05). Shrimp fed diets supplemented with 1.5 or 2 mL of ChNPs/PSO demonstrated significantly higher survival rates compared to other groups. These groups also exhibited significant increases in crude protein, ash content, phagocytic index, superoxide dismutase, and lipase levels (P < 0.05). The ChNPs+PSO2 group showed the highest amylase activity (P < 0.05). Furthermore, shrimp in these groups displayed significantly elevated total hemocyte counts, lysozyme activity, and bactericidal capacity (P < 0.05). Besides, a significant increase in phenoloxidase and phagocytic activities was observed in the ChNPs+PSO1 group (P < 0.05). The expression levels of prophenoloxidase (LvproPO) and hemocyanin (LvHc) genes were significantly increased in the hepatopancreas of shrimp fed diets enriched with 1.5 or 2 mL of ChNPs/PSO. The survival rate after infection with V. parahaemolyticus was higher in all ChNPs/PSO-enriched groups compared to the control group. Dietary supplementation with ChNPs/PSO restored the histological damages in the muscle, hepatopancreas, and intestine induced by V. parahaemolyticus infection. In summary, pumpkin seed oil-loaded chitosan nanoparticles (ChNPs/PSO) hold potential as a feed additive for enhancing growth performance, immune ability, and disease resistance against V. parahaemolyticus in Whiteleg shrimp.http://www.sciencedirect.com/science/article/pii/S2352513424006872Pumpkin seed oil-loaded chitosan nanoparticlesGrowth promoterImmunostimulatoryHistopathologyVibrio parahaemolyticus |
| spellingShingle | El-Sayed Hemdan Eissa Wesam Mohamed Salama Samia Elbahnaswy Mai A.M. El-Son Zienab E. Eldin Sara Amer Layla A. Almutairi Fatma Mohamed Ameen Khalil Safia M.A. Bahshwan Mada M. AL-Qurashi Yasmin M. Abd El-Aziz Moaheda E.H. Eissa Aiman A. Alsaegh Roshmon Thomas Mathew El Sayed Taha Rizk Pumpkin seed oil-loaded chitosan nanoparticles enhance growth, digestive enzymes, and immune response in whiteleg shrimp (Litopenaeus vannamei): Impacts on histopathology and survival against Vibrio parahaemolyticus Aquaculture Reports Pumpkin seed oil-loaded chitosan nanoparticles Growth promoter Immunostimulatory Histopathology Vibrio parahaemolyticus |
| title | Pumpkin seed oil-loaded chitosan nanoparticles enhance growth, digestive enzymes, and immune response in whiteleg shrimp (Litopenaeus vannamei): Impacts on histopathology and survival against Vibrio parahaemolyticus |
| title_full | Pumpkin seed oil-loaded chitosan nanoparticles enhance growth, digestive enzymes, and immune response in whiteleg shrimp (Litopenaeus vannamei): Impacts on histopathology and survival against Vibrio parahaemolyticus |
| title_fullStr | Pumpkin seed oil-loaded chitosan nanoparticles enhance growth, digestive enzymes, and immune response in whiteleg shrimp (Litopenaeus vannamei): Impacts on histopathology and survival against Vibrio parahaemolyticus |
| title_full_unstemmed | Pumpkin seed oil-loaded chitosan nanoparticles enhance growth, digestive enzymes, and immune response in whiteleg shrimp (Litopenaeus vannamei): Impacts on histopathology and survival against Vibrio parahaemolyticus |
| title_short | Pumpkin seed oil-loaded chitosan nanoparticles enhance growth, digestive enzymes, and immune response in whiteleg shrimp (Litopenaeus vannamei): Impacts on histopathology and survival against Vibrio parahaemolyticus |
| title_sort | pumpkin seed oil loaded chitosan nanoparticles enhance growth digestive enzymes and immune response in whiteleg shrimp litopenaeus vannamei impacts on histopathology and survival against vibrio parahaemolyticus |
| topic | Pumpkin seed oil-loaded chitosan nanoparticles Growth promoter Immunostimulatory Histopathology Vibrio parahaemolyticus |
| url | http://www.sciencedirect.com/science/article/pii/S2352513424006872 |
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