Seroprevalence and Risk Factors of Brucellosis in Ruminants in Dhofar Province in Southern Oman
Objective. The aim of the present work was to raise awareness of Brucella infection and emphasize the use of serological tests for screening and confirmation of the presence of the infection in different localities in the Dhofar region in the Sultanate of Oman. Methods. A seroprevalence of Brucella...
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | Veterinary Medicine International |
| Online Access: | http://dx.doi.org/10.1155/2022/3176147 |
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| author | Waleed Al-Marzooqi Elshafie I. Elshafie AlGhalya Al-Toobi Abeer Al-Hamrashdi Kaadhia Al-Kharousi Hatim El-Tahir Maryne Jay Yannick Corde Yasmin ElTahir |
| author_facet | Waleed Al-Marzooqi Elshafie I. Elshafie AlGhalya Al-Toobi Abeer Al-Hamrashdi Kaadhia Al-Kharousi Hatim El-Tahir Maryne Jay Yannick Corde Yasmin ElTahir |
| author_sort | Waleed Al-Marzooqi |
| collection | DOAJ |
| description | Objective. The aim of the present work was to raise awareness of Brucella infection and emphasize the use of serological tests for screening and confirmation of the presence of the infection in different localities in the Dhofar region in the Sultanate of Oman. Methods. A seroprevalence of Brucella infection in naturally infected livestock was undertaken in 50 farms (a total of 434 sera, 207 goats, 84 sheep, 54 cattle, and 89 camels) from different wilayat of the Dhofar region in the southern part of Oman. Rose Bengal (RBT), complement fixation (CFT), and indirect enzyme-linked immunosorbent assay (I-ELISA) tests were used to determine the presence of Brucella antibodies. Statistical analysis (Pearson chi-square, binary logistic regression, and univariate logistic regression) was used to investigate the significance between the prevalence and the categorical risk factors individually, with two or more levels (animal species, animal condition, and or location). Results. Our results show that the overall seroprevalence based on CFT, RBT, and I-ELISA was 3% (13/424, CI: 1.8–5.1%), 4.8% (21/434, CI: 3.1–7.3%), and 8% (35/434, CI: 5.8–10.9%), respectively. The highest seroprevalence was reported in goats (13% (27/207)) and animals from East Jabal (13% (21/161)), whereas the lowest was recorded in camels (3.4% (3/89)) and animals from deserts (1.4% (1/69)). Parameters such as the positive predictive value (PPV) and the negative predictive value (NPV) showed that the sensitivity of I-ELISA and CFT based on the RBT test was 61.9% and 57.1%, respectively, whereas the specificity of I-ELISA (94.6%) was less than that of CFT (97.33%). Conclusion. We concluded that three tests are confirmatory for the presence of Brucella infection. |
| format | Article |
| id | doaj-art-8683d427462348b3bc030d59088ce9ca |
| institution | Kabale University |
| issn | 2042-0048 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine International |
| spelling | doaj-art-8683d427462348b3bc030d59088ce9ca2025-02-03T06:08:44ZengWileyVeterinary Medicine International2042-00482022-01-01202210.1155/2022/3176147Seroprevalence and Risk Factors of Brucellosis in Ruminants in Dhofar Province in Southern OmanWaleed Al-Marzooqi0Elshafie I. Elshafie1AlGhalya Al-Toobi2Abeer Al-Hamrashdi3Kaadhia Al-Kharousi4Hatim El-Tahir5Maryne Jay6Yannick Corde7Yasmin ElTahir8Sultan Qaboos UniversitySultan Qaboos UniversitySultan Qaboos UniversitySultan Qaboos UniversitySultan Qaboos UniversityMinistry of Agriculture & FisheriesParis-Est University/AnsesParis-Est University/AnsesSultan Qaboos UniversityObjective. The aim of the present work was to raise awareness of Brucella infection and emphasize the use of serological tests for screening and confirmation of the presence of the infection in different localities in the Dhofar region in the Sultanate of Oman. Methods. A seroprevalence of Brucella infection in naturally infected livestock was undertaken in 50 farms (a total of 434 sera, 207 goats, 84 sheep, 54 cattle, and 89 camels) from different wilayat of the Dhofar region in the southern part of Oman. Rose Bengal (RBT), complement fixation (CFT), and indirect enzyme-linked immunosorbent assay (I-ELISA) tests were used to determine the presence of Brucella antibodies. Statistical analysis (Pearson chi-square, binary logistic regression, and univariate logistic regression) was used to investigate the significance between the prevalence and the categorical risk factors individually, with two or more levels (animal species, animal condition, and or location). Results. Our results show that the overall seroprevalence based on CFT, RBT, and I-ELISA was 3% (13/424, CI: 1.8–5.1%), 4.8% (21/434, CI: 3.1–7.3%), and 8% (35/434, CI: 5.8–10.9%), respectively. The highest seroprevalence was reported in goats (13% (27/207)) and animals from East Jabal (13% (21/161)), whereas the lowest was recorded in camels (3.4% (3/89)) and animals from deserts (1.4% (1/69)). Parameters such as the positive predictive value (PPV) and the negative predictive value (NPV) showed that the sensitivity of I-ELISA and CFT based on the RBT test was 61.9% and 57.1%, respectively, whereas the specificity of I-ELISA (94.6%) was less than that of CFT (97.33%). Conclusion. We concluded that three tests are confirmatory for the presence of Brucella infection.http://dx.doi.org/10.1155/2022/3176147 |
| spellingShingle | Waleed Al-Marzooqi Elshafie I. Elshafie AlGhalya Al-Toobi Abeer Al-Hamrashdi Kaadhia Al-Kharousi Hatim El-Tahir Maryne Jay Yannick Corde Yasmin ElTahir Seroprevalence and Risk Factors of Brucellosis in Ruminants in Dhofar Province in Southern Oman Veterinary Medicine International |
| title | Seroprevalence and Risk Factors of Brucellosis in Ruminants in Dhofar Province in Southern Oman |
| title_full | Seroprevalence and Risk Factors of Brucellosis in Ruminants in Dhofar Province in Southern Oman |
| title_fullStr | Seroprevalence and Risk Factors of Brucellosis in Ruminants in Dhofar Province in Southern Oman |
| title_full_unstemmed | Seroprevalence and Risk Factors of Brucellosis in Ruminants in Dhofar Province in Southern Oman |
| title_short | Seroprevalence and Risk Factors of Brucellosis in Ruminants in Dhofar Province in Southern Oman |
| title_sort | seroprevalence and risk factors of brucellosis in ruminants in dhofar province in southern oman |
| url | http://dx.doi.org/10.1155/2022/3176147 |
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