Identification of Plasma Absorbed Components of Qingxin Zishen Prescription by UPLC-Q-TOF MS
It has been confirmed in clinic that Qingxin Zishen Prescription (QZP) is effective and safe in relieving vasomotor symptoms. However, its active components have not been elucidated. In this study, a method based on ultra-performance liquid chromatography quadrupole-time of flight mass spectrometry...
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Editorial Board of Journal of Chinese Mass Spectrometry Society
2025-01-01
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| Series: | Zhipu Xuebao |
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| author | Yu HU Juan SHANG Xue-jiao LI Yun-hua FENG Kai-cheng XIE Wei-feng YAO Jian-dong ZOU Yun CHEN Su LU Mei-juan XU |
| author_facet | Yu HU Juan SHANG Xue-jiao LI Yun-hua FENG Kai-cheng XIE Wei-feng YAO Jian-dong ZOU Yun CHEN Su LU Mei-juan XU |
| author_sort | Yu HU |
| collection | DOAJ |
| description | It has been confirmed in clinic that Qingxin Zishen Prescription (QZP) is effective and safe in relieving vasomotor symptoms. However, its active components have not been elucidated. In this study, a method based on ultra-performance liquid chromatography quadrupole-time of flight mass spectrometry (UPLC-Q-TOF MS) was developed to identify the plasma absorbed components of QZP. After continuous intragastric administration of 48.5 g/kg QZP for 3 days to female SD rats, plasma samples were collected and then pre-treated by solid phase extraction (SPE) to remove proteins in plasma. Then, chromatographic separation of the plasma samples was performed on an Agilent ZORBAX SB C18 column (4.6 mm×100 mm, 1.8 µm) using a gradient elution with a mobile phase composed of 0.1% formic acid aqueous solution (A) and methanol-acetonitrile (1:1, V/V, B) prior to MS analysis. The column temperature was kept at 40 ℃. The MS and MS2 analysis were performed with the electrospray ionization (ESI) source under both positive and negative ion modes from m/z 100 to 1300. Intelligent dynamic background deduction, information dependent acquisition and highly sensitive mode were used to collect the data. According to the accurate m/z values of MS and MS2 information, data from reference substance and related literature, the absorbed components in plasma were identified. In total, forty prototypes from QZP are identified and inferred in the plasma samples, including 27 alkaloids, 9 iridoid glycosides, 2 flavonoid carbon glycosides, 1 phenolic acid and 1 fatty acid. Among them, alkaloids are mainly from Nelumbo nucifera Gaertn, Coptis chinensis Franch, Ziziphus jujuba Mill. var. spinosa (Bunge) Hu ex H. F. Chou, and Uncaria rhynchophylla (Miq.) Miq. ex Havil. Iridoid glycosides are mainly from Rehmannia glutinosa Libosch. and Cornus officinalis Sieb. et Zucc. Furthermore, the fragmentation pathways and characteristic fragment ions of iridoid glycosides and dibenzylisoquinoline alkaloids were summarized. This study provides not only a rapid and comprehensive analysis method for the plasma absorbed components of QZP, but also a scientific fundamental for elucidating the material basis of action of QZP. |
| format | Article |
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| institution | Kabale University |
| issn | 1004-2997 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Editorial Board of Journal of Chinese Mass Spectrometry Society |
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| spelling | doaj-art-86767db3efcd4bfb8f09f9cb5ba7bdc02025-01-22T02:33:54ZengEditorial Board of Journal of Chinese Mass Spectrometry SocietyZhipu Xuebao1004-29972025-01-01461486410.7538/zpxb.2024.00582024-058-c13Identification of Plasma Absorbed Components of Qingxin Zishen Prescription by UPLC-Q-TOF MSYu HU0Juan SHANG1Xue-jiao LI2Yun-hua FENG3Kai-cheng XIE4Wei-feng YAO5Jian-dong ZOU6Yun CHEN7Su LU8Mei-juan XU9Jiangsu Provincial Hospital of Traditional Chinese Medicine, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210029, ChinaJiangsu Provincial Hospital of Traditional Chinese Medicine, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210029, ChinaJiangsu Provincial Hospital of Traditional Chinese Medicine, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210029, ChinaJiangsu Provincial Hospital of Traditional Chinese Medicine, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210029, ChinaJiangsu Provincial Hospital of Traditional Chinese Medicine, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210029, ChinaNanjing University of Chinese Medicine, Nanjing 210023, ChinaJiangsu Provincial Hospital of Traditional Chinese Medicine, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210029, ChinaJiangsu Provincial Hospital of Traditional Chinese Medicine, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210029, ChinaJiangsu Provincial Hospital of Traditional Chinese Medicine, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210029, ChinaJiangsu Provincial Hospital of Traditional Chinese Medicine, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210029, ChinaIt has been confirmed in clinic that Qingxin Zishen Prescription (QZP) is effective and safe in relieving vasomotor symptoms. However, its active components have not been elucidated. In this study, a method based on ultra-performance liquid chromatography quadrupole-time of flight mass spectrometry (UPLC-Q-TOF MS) was developed to identify the plasma absorbed components of QZP. After continuous intragastric administration of 48.5 g/kg QZP for 3 days to female SD rats, plasma samples were collected and then pre-treated by solid phase extraction (SPE) to remove proteins in plasma. Then, chromatographic separation of the plasma samples was performed on an Agilent ZORBAX SB C18 column (4.6 mm×100 mm, 1.8 µm) using a gradient elution with a mobile phase composed of 0.1% formic acid aqueous solution (A) and methanol-acetonitrile (1:1, V/V, B) prior to MS analysis. The column temperature was kept at 40 ℃. The MS and MS2 analysis were performed with the electrospray ionization (ESI) source under both positive and negative ion modes from m/z 100 to 1300. Intelligent dynamic background deduction, information dependent acquisition and highly sensitive mode were used to collect the data. According to the accurate m/z values of MS and MS2 information, data from reference substance and related literature, the absorbed components in plasma were identified. In total, forty prototypes from QZP are identified and inferred in the plasma samples, including 27 alkaloids, 9 iridoid glycosides, 2 flavonoid carbon glycosides, 1 phenolic acid and 1 fatty acid. Among them, alkaloids are mainly from Nelumbo nucifera Gaertn, Coptis chinensis Franch, Ziziphus jujuba Mill. var. spinosa (Bunge) Hu ex H. F. Chou, and Uncaria rhynchophylla (Miq.) Miq. ex Havil. Iridoid glycosides are mainly from Rehmannia glutinosa Libosch. and Cornus officinalis Sieb. et Zucc. Furthermore, the fragmentation pathways and characteristic fragment ions of iridoid glycosides and dibenzylisoquinoline alkaloids were summarized. This study provides not only a rapid and comprehensive analysis method for the plasma absorbed components of QZP, but also a scientific fundamental for elucidating the material basis of action of QZP.https://zpxb.xml-journal.net/article/doi/10.7538/zpxb.2024.0058qingxin zishen prescriptionultra-performance liquid chromatography quadrupole-time of flight mass spectrometry (uplc-q-tof ms)absorbed plasma componentsalkaloidsiridoid glycosidesfragmention pathway |
| spellingShingle | Yu HU Juan SHANG Xue-jiao LI Yun-hua FENG Kai-cheng XIE Wei-feng YAO Jian-dong ZOU Yun CHEN Su LU Mei-juan XU Identification of Plasma Absorbed Components of Qingxin Zishen Prescription by UPLC-Q-TOF MS Zhipu Xuebao qingxin zishen prescription ultra-performance liquid chromatography quadrupole-time of flight mass spectrometry (uplc-q-tof ms) absorbed plasma components alkaloids iridoid glycosides fragmention pathway |
| title | Identification of Plasma Absorbed Components of Qingxin Zishen Prescription by UPLC-Q-TOF MS |
| title_full | Identification of Plasma Absorbed Components of Qingxin Zishen Prescription by UPLC-Q-TOF MS |
| title_fullStr | Identification of Plasma Absorbed Components of Qingxin Zishen Prescription by UPLC-Q-TOF MS |
| title_full_unstemmed | Identification of Plasma Absorbed Components of Qingxin Zishen Prescription by UPLC-Q-TOF MS |
| title_short | Identification of Plasma Absorbed Components of Qingxin Zishen Prescription by UPLC-Q-TOF MS |
| title_sort | identification of plasma absorbed components of qingxin zishen prescription by uplc q tof ms |
| topic | qingxin zishen prescription ultra-performance liquid chromatography quadrupole-time of flight mass spectrometry (uplc-q-tof ms) absorbed plasma components alkaloids iridoid glycosides fragmention pathway |
| url | https://zpxb.xml-journal.net/article/doi/10.7538/zpxb.2024.0058 |
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