Deep eutectic solvent extraction and biological activity of polysaccharides from Tenebrio molitor
This study aimed to extract polysaccharides from Tenebrio molitor using ultrasound-assisted deep eutectic solvent (DESs) and to evaluate their structural features, as well as their antimicrobial, antioxidant, and α-amylase inhibitory activities. Various DESs were tested for polysaccharides extractio...
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| Language: | English |
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Elsevier
2025-01-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844025001707 |
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| author | Shengru Yang Xu Li Qiaoli Li |
| author_facet | Shengru Yang Xu Li Qiaoli Li |
| author_sort | Shengru Yang |
| collection | DOAJ |
| description | This study aimed to extract polysaccharides from Tenebrio molitor using ultrasound-assisted deep eutectic solvent (DESs) and to evaluate their structural features, as well as their antimicrobial, antioxidant, and α-amylase inhibitory activities. Various DESs were tested for polysaccharides extraction, and the process was optimized using response surface methodology (RSM). A preliminary structural analysis of the polysaccharides was conducted using infrared spectrum. The DESs were characterized by measuring their pH, viscosity, conductivity, refractive index, and density. The optimal extraction agent and parameters were determined. Significant differences in pH, viscosity, and conductivity were observed among DESs, whereas differences in refractive index and density were not significant. Choline chloride-lactic acid was identified as the optimal extraction agent. The optimal extraction parameters were a DES molar ratio of 1:2.1, a water content of 27 %, an extraction temperature of 70 °C, and an extraction time of 44 min, resulting in polysaccharides yield of 18.62 %. The extracted polysaccharides exhibited strong inhibitory effects against Salmonella, along with antioxidant activity and α-amylase inhibitory activities. The study demonstrated that polysaccharides from Tenebrio molitor can be efficiently extracted using DESs, showcasing significant biological activities, including antibacterial, antioxidant, and α-amylase inhibitory properties. These findings highlight the potential applications of Tenebrio molitor polysaccharides as valuable biological resources. |
| format | Article |
| id | doaj-art-86514a2ab98b4cff95398516c745e178 |
| institution | Kabale University |
| issn | 2405-8440 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj-art-86514a2ab98b4cff95398516c745e1782025-02-02T05:28:12ZengElsevierHeliyon2405-84402025-01-01112e41790Deep eutectic solvent extraction and biological activity of polysaccharides from Tenebrio molitorShengru Yang0Xu Li1Qiaoli Li2Corresponding author. The First Affiliated Hospital of Henan University, 357 Ximen Road, Kaifeng, 475000, Henan Province, PR China.; The First Affiliated Hospital of Henan University, Kaifeng, 475000, PR ChinaThe First Affiliated Hospital of Henan University, Kaifeng, 475000, PR ChinaThe First Affiliated Hospital of Henan University, Kaifeng, 475000, PR ChinaThis study aimed to extract polysaccharides from Tenebrio molitor using ultrasound-assisted deep eutectic solvent (DESs) and to evaluate their structural features, as well as their antimicrobial, antioxidant, and α-amylase inhibitory activities. Various DESs were tested for polysaccharides extraction, and the process was optimized using response surface methodology (RSM). A preliminary structural analysis of the polysaccharides was conducted using infrared spectrum. The DESs were characterized by measuring their pH, viscosity, conductivity, refractive index, and density. The optimal extraction agent and parameters were determined. Significant differences in pH, viscosity, and conductivity were observed among DESs, whereas differences in refractive index and density were not significant. Choline chloride-lactic acid was identified as the optimal extraction agent. The optimal extraction parameters were a DES molar ratio of 1:2.1, a water content of 27 %, an extraction temperature of 70 °C, and an extraction time of 44 min, resulting in polysaccharides yield of 18.62 %. The extracted polysaccharides exhibited strong inhibitory effects against Salmonella, along with antioxidant activity and α-amylase inhibitory activities. The study demonstrated that polysaccharides from Tenebrio molitor can be efficiently extracted using DESs, showcasing significant biological activities, including antibacterial, antioxidant, and α-amylase inhibitory properties. These findings highlight the potential applications of Tenebrio molitor polysaccharides as valuable biological resources.http://www.sciencedirect.com/science/article/pii/S2405844025001707PolysaccharidesTenebrio molitorAntioxidantResponse surfaceDeep eutectic solvents |
| spellingShingle | Shengru Yang Xu Li Qiaoli Li Deep eutectic solvent extraction and biological activity of polysaccharides from Tenebrio molitor Heliyon Polysaccharides Tenebrio molitor Antioxidant Response surface Deep eutectic solvents |
| title | Deep eutectic solvent extraction and biological activity of polysaccharides from Tenebrio molitor |
| title_full | Deep eutectic solvent extraction and biological activity of polysaccharides from Tenebrio molitor |
| title_fullStr | Deep eutectic solvent extraction and biological activity of polysaccharides from Tenebrio molitor |
| title_full_unstemmed | Deep eutectic solvent extraction and biological activity of polysaccharides from Tenebrio molitor |
| title_short | Deep eutectic solvent extraction and biological activity of polysaccharides from Tenebrio molitor |
| title_sort | deep eutectic solvent extraction and biological activity of polysaccharides from tenebrio molitor |
| topic | Polysaccharides Tenebrio molitor Antioxidant Response surface Deep eutectic solvents |
| url | http://www.sciencedirect.com/science/article/pii/S2405844025001707 |
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